蜂胶有效成分醇液两步提取法的研究

采用微波技术与传统浸提法相结合，研究出一种新式蜂胶有效成分提取方法——醇液两步提取法。给出该法的最佳提取工艺条件，其最大提取率为88.71%，实现了快速高效的提取效果。     

成排连铸坯端面中心坐标视觉自动提取方法

为了实现成排连铸坯端面机器人贴标时各连铸坯端面中心坐标的快速提取,提出了先提取连铸坯端面图像角点像面坐标,再计算各连铸坯端面中心像面坐标的研究方案。首先,提出了一种改进型SUSAN角点检测算法,解决了图像中相邻连铸坯端面图像边界间距离过小和连铸坯端面图像角为弧形角所造成的角点漏检问题;然后,提取角点的像面坐标,并确定各封闭区域所包含连铸坯端面个数;最后,采用一种倾斜连铸坯端面图像中心像面坐标的提取方法,计算各连铸坯端面中心像面坐标。应用以上方法进行成排连铸坯端面机器人贴标实验,实验结果表明,连铸坯端面水平和竖直方向贴标位置误差范围分别为-0.625～0.850 mm和-0.550～0.875 mm,完全满足企业对多根连铸坯端面自动贴标位置误差允许值1 mm的要求。所提视觉方法在理论和实际应用上都是可行的,不仅为连铸坯端面贴标机器人提供了准确的贴标位置,而且为矩形图像元素的中心坐标提取提供了一种可靠的方法,具有一定的应用价值。     

粗蒽中提取菲的研究

研究了从粗蒽中提取菲的方法 ,即用乙二醇对原料进行共沸 ,以四氯化碳为溶剂并用硫酸处理 ,最后用无水乙醇重结晶 ,制定出最佳的工艺路线 .     

基于自组织特征映射的矢量量化方法

对自组织特征映射神经网络的特性进行分析，并将其与矢量量化问题的实质进行比较，提出了一个实现矢量量化的自组织特征映射算法。分析与实验表明，该算法是稳定收敛的。算法的学习结果与网络的初始状态无关，并且十分接近于全局最优解的下限。将该算法应用于图像数据的压缩，取得了很好的结果     

Prominent feature selection of microarray data

For wavelet transform, a set of orthogonal wavelet basis aims to detect the localized changing features contained in microarray data. In this research, we investigate the performance of the selected wavelet features based on wavelet detail coefficients at the second level and the third level. The genetic algorithm is performed to optimize wavelet detail coefficients to select the best discriminant features. Experiments are carried out on four microarray datasets to evaluate the performance of classification. Experimental results prove that wavelet features optimized from detail coefficients efficiently characterize the differences between normal tissues and cancer tissues.     

贵州南方菜豆花叶病毒的ELISA检疫

采用ELISA间接法对分布于贵州几个地区的菜豆进行南方菜豆花叶病毒的检测 ,结果表明 :在所采集到的样品中 ,仅贵阳地区的菜豆感染有南方菜豆花叶病毒。其检测的灵敏度和最佳反应稀释度分别为 80 6ng/mL和 1∶5 0 0稀释度。     

H2株甲肝减毒活疫苗K7的核苷酸全序列分析

应用ＲＴ－ＰＣＲ获取Ｈ２株甲肝减毒活疫苗（Ｋ７）的ｃＤＮＡ片段，经末端终止测序ＰＣＲ试剂盒和全自动测序仪（ＡＢＩ３７７）作序列测定，用Ｍａｘｔｒｉｇｅｎｅ软件进行计算机分析，Ｋ７疫苗病毒的核苷酸全长含７４４３个碱基．Ｋ７对比其亲代Ｈ２株，减毒的ＨＭ１７５株以及ＨＭ１７５野毒株，同源性分别为９９．７５％，９９．９５％和９９．６１％；彼此间在Ｐ１和Ｐ２连接区１６８个碱基的同源性为９９．４％～１００％，均为ⅠＢ基因亚型．前三者在５’非编码区均有１３１～１３４位和２０３位５个碱基缺失，且结构蛋白基因区序列完全一致．本文用分子病毒学方法证实了Ｋ７疫苗的优异纯毒水平，也为开展甲型肝炎分子流行病学工作提供了基础资料     

蛋黄卵磷脂制备工艺探讨

对蛋黄卵磷脂的制备方法作了介绍，并对其工艺条件特别是超临界二氧化碳萃取的工艺条件进行了讨论。     

Thymidine kinase gene mutation leads to reduced virulence of pseudorabies virus

To explore correlation between the tk gene structure of pseudorabies virus (PRV) and its virulence, to study the effect of the gene mutation on PRV biological properties, and to investigate mechinism of reduced virulence, thymidine kinase (TK)-deficient mutant of pseudorabies virus strain Hubei (PRV HB) was isolated by selection for resistance to 5-bromodeoxyuridine. The tk genes of PRV HB and its TK^― mutant were cloned and sequenced. 1587 base pairs of the tk gene and flanking regions of wild-type (wt) virus were sequenced, which included an open reading frame (ORF) of 1098 bp encoding a protein of 366 amino acids. The ORF contained two 137-bp repeated sequences, which were connected by an adenosine. 1458 bp of the tk and flanking regions of TK^― mutant were sequenced. Analysis of the tk gene sequence of TK^― mutant indicated that one of 137 bp repeated sequence and the connecting adenosine in the tk gene of the wt virus was deleted and a repeated sequence of 8 nucleotides (GCGCGCC) was inserted. All other nucleotides of TK^―mutant were identical to that of wt virus. Deletion and insertion of the nucleotide sequence resulted in a frameshift and a premature chain termination, and the resultant TK protein was not active. Analysis of the amino acid sequence revealed that TK protein of PRV HB contained the conserved consensus sequence of herpesviral TKs and an additional conserved-DHR-motif. The results of this work also indicated that TK^― mutant was genetically stable. Compared to PRV HB, virulence of TK^― mutant was greatly decreased. Mice vaccinated with TK^― mutant were completely protected against a lethal challenge with virulent PRV (HB).     

光合细菌中类胡萝卜素的提取及性质的研究

确立了光合细菌ＰＳＢ－Ｈ 的最佳提取工艺条件为：以７∶２ 的丙酮－甲醇液为溶剂，用量２５ｍ Ｌ／４０ｍ Ｌ发酵液，２ ｈ，一次浸提，再经进一步的分离和纯化，得到类胡萝卜素纯品。并对提取后的类胡萝卜素的性质进行了进一步的探讨：具有双键烯烃的一般性质，耐热性较好，需置于暗处保存，在紫外防护试验中，当其质量浓度达到０．１ ｍ ｇ／Ｌ时，就有显著的防护作用