颗粒体病毒对小菜蛾种群控制作用的评价

在组建小菜蛾自然种群生命表的基础上，应用种群趋势指数和干扰作用控制指数，评价小菜蛾颗粒体病毒对小菜蛾种群的控制作用．试验结果表明：施用颗粒体病毒制剂后，其种群趋势指数为5．99，干扰作用控制指数为023．即，其下代数量明显下降．可见，小菜蛾颗粒体病毒对小菜蛾种群的控制作用是明显的．     

斜纹夜蛾NPV染病幼虫发育与温度的关系

在不同温度下，以不同剂量的ＳｌＮＰＶ饲食斜纹夜蛾ＳｐｏｄｏｐｔｅｒａｌｉｔｕｒａＦ．幼虫．结果表明，１～６龄染病幼虫的发育起点温分别是１８．７９，１６．２９，１４．１９，１０．８７，８．３９，７．０３℃；１～４龄染病幼虫的发育最适温分别是３２．８５，３３．４８，３４．２２，３１．７５℃．１～６龄染病幼虫的取食最适温分别是２６．５，２５．５７，２７．９０，２７．８２，２９．０１，２８．８５℃．建立了１～６龄染病幼虫取食白菜量的温度依赖模型，死亡速率模型，以及依赖于温度和ＳｌＮＰＶ饲食量的发育速率模型     

一种简便提纯白斑综合症病毒核衣壳的方法

通过人工感染 ,获得具典型白斑综合症的病虾 ,取病虾的鳃和胃组织冰浴匀浆 ,通过 3次冷冻差速离心 ,获得大量较纯的病毒核衣壳 ,其大小为 (5 8～ 75 )nm× (36 0～ 42 0 )nm。     

Tobacco curly shoot virus iso-lated in Yunnan is a distinct species of Begomovirus

Virus isolate Y1 was obtained from tobacco showing curly shoot symptoms in Baoshan, Yunnan Province. Whitefly transmission test and virion morphology observation showed that it is a begomovirus. In reactions with 14 monoclonal antibodies raised against begomoviruses, Y1 was readily differentiated from begomoviruses reported in China, Pakistan and India. The complete nucleotide sequence of DNA-A was determined, it contains 2746 nucleotides, with two ORFs in virion-sense DNA and four ORFs in complementary-sense DNA. Comparisons with total DNA-A, intergenic region and deduced amino acid sequences of individual ORFs showed that Y1 is a distinct Begomovirus species, for which the name Tobacco curly shoot virus (TCSV) is proposed. The total DNA-A of TCSV is most closely related to that of Tomato leaf curl virus from India (85% sequence identity). In contrast, the deduced coat protein of TCSV is most like that of Cotton leaf curl virus 72b isolate from Pakistan (98% amino acid sequence identity).     

Characterization and relative abundance of maxi-chloride channels in Epstein-Barr virus (EBV) producer: B95-8 cells

Several Epstein-Barr virus (EBV)-transformed cell lines were used to investigate the pathogenesis of lymphoproliferative diseases and nasopharyngeal carcinoma. The studies focus on the events occurring inside the membrane. On only one occasion, the cell membrane of EBV-transformed B lymphocytes from a cystic fibrosis patient was found to express defective Cl channels (CFTR; Cystic Fibrosis Transmembrane conductance Regulator), as in the airway epithelial cell. No other type of channel in EBV-transformed cells has so far been investigated. In this study, the cell membrane of the B95-8 cell was examined by the patch-clamp technique and compared to the non-EBV-infected BJAB cell. The high conductance (300 pS) maxi-chloride (Cl) channel activity was the most frequently observed event in inside-out configurations. Under similar experimental conditions, we have found a significantly higher probability of detecting maxi-Cl channel activity on the cell membrane of B95-8 cells (69%) than on BJAB cells (27%), or as previously reported on resting murine B lymphocytes (38%) or intact human T lymphocytes (37%). The relative abundance of the maxi-Cl channel on B95-8 cells may be linked to EBV infection and/or secretory ability.     

光照对心叶烟叶绿素含量及抑制烟草花叶病毒侵染的影响

在不同光照强度处理下,测定了心叶烟叶片中叶绿素含量及接种烟草花叶病毒后生成的枯斑数,结果表明:随着光照强度的降低,叶绿素含量逐渐减少,而半叶平均枯斑数呈增加的趋势,两者之间具有负相关性,且相关系数r=-0.95.     

马铃薯Y病毒属病毒基因功能研究进展

马铃薯Y病毒属是植物病毒中最大的属。近年来马铃薯Y病毒属功能基因组学取得了很大进展。本文综述了在病毒系统侵染、蚜虫和种子传毒、症状形成等方面的研究结果。     

关于网络安全问题的研究

介绍了网络安全的概念,分析了Internet中存在的主要安全隐患,重点讨论了网络防火墙技术、加密技术、PKI技术、虚拟专用网技术和安全隔离技术的主要特征及其应用。     

Generation of VP5 deficient mutant of infectious bursal disease virus strain HZ2

Infectious bursal disease virus (IBDV) is one of the most significant viral pathogens in chickens[1]. It causes high mortality in young chickens and establishes an immunosuppression state by destroying the precursorsInfectious bursal disease virus (IBDV) …     

利用柑橘茎段诱导不定芽改进微芽嫁接技术的研究

试验以成年态纽荷尔脐橙茎段为外植体，诱导产生不定芽改进微芽嫁接成活率与脱毒率．结果表明：半木质化茎段作为培养外植体污染率最低，不定芽诱导的最佳培养基为MT＋＋6-BA1．0mg／L＋NAA0．2mg／L＋GA30．2mg／L．将诱导的嫩芽进行微芽嫁接，成活率超过80．8％，对照成活率为61．3％，聚合酶链式反应（PCR）检测脱毒率达100％．