Discovery of a male-biased mutant family and identification of a male-specific SCAR marker in gynogenetic gibel carp Carassius auratus gibelio

Gibel carp (Carassius auratus gibelio) is a uniquely gynogenetic species with a minor ratio of males in natural habitats, but its male origin and sex determination mechanisms have been unknown. In this study, a male-biased mutant family was discovered from the gynogenetic gibel carp, and a male-specific SCAR marker was identified from the mutant family. Normal spermatogenesis was observed in the male testes by immunofluorescence histochemistry. Nearly identical AFLP profiles were observed between males and females, but a male-specific 86 bp AFLP fragment was screened by sex-pool bulked segregant analysis and individual screening. Based on the male-specific AFLP fragment, a total of 579 bp sequences were cloned by genome walking. Subsequently, a male-specific SCAR marker was designed, and the male-specific DNA fragment was confirmed to be steadily transmitted to the next generation and consistently detected only in males.     

结构参数变化对自锚式悬索桥动力特性的影响

以广州已建的主跨219m的自锚式悬索桥为例,运用大型有限元分析软件ANSYS建立了模型,在结构参数变化时,对该桥动力特性进行了计算分析,并得出一些结论。     

生态平衡与语种保护

呼吁人们要用保护濒临灭绝动植物的热情,保护少数民族语言文字,创造少数民族语文氛围,继承和发扬少数民族文化。     

安哥拉山羊改良建昌黑山羊高代杂种羊血液生化遗传标记的研究

采用ＰＡＧＥ法检测Ｆ３的Ｈb、Ｔf、Hp,Pr,Alb、Po、ＬＤＨ、Ａkp和Amy等基因座的多态性和相关分析。研究结果表明,Ｆ３的Hb、Ｔf、Hp、Ａkp呈多态,Ａlb、Pr、Ｐr、Ｐo、ＬＤＨ、Ａmy呈单态;ＬＤＨ同工酶相对活力顺序为ＬＤＨ１＞ＬＤＨ３＞ＬＤＨ２＞ＬＤＨ４＞ＬＤＨ５;Ｆ３与安哥拉山羊Ａkp基因型分布差异显著（Ｐ＜０．０５）,与建昌黑山羊差异极显著（Ｐ＜０．０１）;平均基因杂合度安哥拉山羊最高,Ｆ３次之,建昌黑山羊最低,Ｆ３与安哥拉山羊的遗传相关系数大,遗传距离小;ＬＤＨ１的相对活力与净毛率呈极负相关（Ｐ＜０．０１）,ＬＤＨ５与两型毛根数百分比呈显著正相关（Ｐ＜０．０５）、与两型毛重量百分比呈极显著正相关（Ｐ＜０．０１）、、与无无髓毛伸度呈极显著负相关（Ｐ＜０．０１）,ＬＤＨ其他各要带的相对活力与产毛性状无显著相关。     

中国木兰属一新种

本发表了中国木兰属一新种,即鸡公玉兰Ｍａｇｎｏｌｉａ ｊｉｇｏｎｇｓｈａｎｅｎｓｉｓ Ｔ．Ｂ．Ｚｈａｏ,Ｄ．Ｌ．Ｆｕ ｅｔ Ｗ．Ｂ．Ｓｕｎ,ｓｐ．ｎｏｖ．,本新种与罗田玉兰Ｍ．ｐｉｌｏｃａｒｐａ Ｚ．Ｚ．Ｚｈａｏ ｅｔ Ｚ．Ｗ．Ｘｉｅ近似,但区别,幼枝淡黄绿色,被短柔毛,叶薄革质、革质,侧脉５－９对,背面被弯曲短柔毛,具７种叶型：①宽椭圆形或宽卵形,先端凹裂,似凹叶厚朴;②侧卵形,先端凹似凹叶森     

甘薯毛状根植株再生研究

用发根农杆菌Ｒ１０００菌株感梁甘薯（渝薯３４品种）,获得转化毛状根,从毛状根中选择出了５个无性系,当毛状根培养在含有２ｍｇ／Ｌ６－ＢＡ＋１ｍｏｌ／Ｌ玉米素的ＭＳ培养基上诱导出了愈伤组织,当愈伤组织培养在含有１ｍｇ／ＬＫＴ＋１ｍｇ／Ｌ６－ＢＡ＋０．５ｍｇ／ＬＩＡＡ的培养基上,３周以后获得了再生植株,用随机及增多态性ＤＮＡ（ＲＡＰＤ）分子标记检测出了再生植株的多态笥。     

DNA标记技术在动物遗传育种中的应用

文中对几种ＤＮＡ标记技术在动物遗传育种领域应用的研究进展作了系统阐述。     

33个杨柳品种指纹图谱构建

[目的]构建杨树与柳树优良品种的指纹图谱,对不同品种进行准确鉴定.[方法]利用柳树EST-SSR标记对33个杨树与柳树优良品种进行通用性检测及基因分型,通过核心引物间的组合构建品种指纹图谱.同时采用非加权组平均法进行聚类,分析各品种间亲缘关系.[结果]12个EST-SSR标记共扩增出97条等位片段,每个位点等位基因数5...     

大米精度与色泽品种水分的关系

本文介绍了在粮食检验中大米的加工精度与色泽,品种和水分的关系及鉴别方法。     

平移同态半群的一些必要条件

给出了平移同态半群的一些必要条件及与内平移同态半群的区别与联系．