品种中心灰靶布局理论在作物布局上的应用

利用品种中心灰靶布局理论的原理和方法对1999—2000年青海省蚕豆区域试验结果进行了分析。结果表明：该方法由于统筹考虑了多个性状，故可得出一个综合性的较高的结论，克服了方差分析、回归分析等芬析方法在作物布局分析上的缺点。表5，参4。     

Characterization, development and exploitation of EST-derived microsatellites in Gossypium raimondii Ulbrich

COTTON IS AN IMPORTANT GLOBAL CASH CROP. IN THE RECENTYEARS, MOLECULAR MARKER TECHNOLOGY HAS BEEN WIDELY APPLIED TO SUCH STUDIES ON COTTON AS GENETIC MAP- PING[1―4], VARIETY PURITY DETECTION[5], GENETIC DIVERSITY ANALYSIS[6], MOLECULAR MARKER-ASSISTED BR…     

菟丝子鉴定方法的研究进展

本文从形态标记、细胞标记、生化标记、分子标记等技术的研究状况方面；概述了菟丝子属近年来的研究进展；分析比较了各鉴定技术的特点；探讨了分子标记在菟丝子近缘种鉴定中的应用前景，并对菟丝子快速检疫鉴定技术作了展望。     

遗传物质稳定性的辩证理解--谈DNA的稳定性与可变性

DNA在生物界中,堪称生物大分子中最重要者,主宰一切生物体维持其生命的各种机能的正常运行及每个物种一代一代繁衍于世,其结构是相对稳定的.     

黄皮尖椒品种比较试验

对引进 9个黄皮尖椒品种的植物学性状、商品性状、抗病性及丰产性进行了比较试验 .结果表明 :苗丰 2 2号植株长势旺 ,产量高 (2 0 6 5 1kg·hm- 2 ) ,抗病性强 ,果实商品性好 ;9919较耐高温 ,商品外观好 ,果较长 ,产量较高 (2 0 0 87kg·hm- 2 ) ;优美 5号产量中等 ,但果色黄绿 ,果形美观 ,易为市场接受 ;茂椒 4号果色黄绿 ,果形美观 ,单果重达 4 5 .0 0g ,极具市场竞争力 .认为这 4个品种可作为海南冬季主栽辣椒品种并加以推广 .     

Genetic Diversity of Three Aristichthys nobilis Populations and One Inbreeding Stock

Genetic diversity of three Aristichthys nobilis populations and one inbreeding stock was studied using random amplified polymorphic DNA(RAPD)method. Materials of natural populations were collected from two counties of Guangxi Province, Hengxian (NH) and Shanglin (NS) and one city of Hubei Province, Wuhan city.(NW). Shanglin and Hengxian's river system respectively belongs to Qianjiang and Yujiang River, two tributaries confluencing to Xunjiang River, main tributary of Xijiang River, which is the biggest tributary of the Zhujiang River system, and Wuhan's water system belongs to the Yangtse River system. The inbreeding stock (RS) was the more than 10 generation descendant by brother-sister mating system whose parents were collected from Shanglin. The results showed that the genetic variety of individuals of RS was very low (0.105 4), whereas that of natural populations was relatively high,which from high to low was 0.158 1 (NW), 0.132 0 (NH) and 0.110 5 (NS). As an index for genetic distance pair-wise populations, the genetic variety between populations was studied, which characterized the genetic distance between populations. The genetic distance of NW and NH, NW and NS, NW and RS were respectively high, whereas that of NH and NS, NS and RS, NH and RS was low. Chi-test(χ2) and the analysis of the genetic variety pair-wise populations was taken as efficient approach for studying population difference.     

滩羊体大品系遗传标记的研究

采用RAPD(Rondom Amplified Polymorphic DNA)技术,利用混合基因池(DNA pool)法,对滩羊体大品系、普通品系进行了DNA多态性分析,从100种具有10个碱基的随机引物中,筛选出84种引物在滩羊群体基因组中共扩增出358条带,其中22种引物的扩增产物表现为多态(占22％),且扩增出32条有差异的条带,占总带数的8.94％;62种引物的扩增产物表现为单态(占62％)。滩羊体大品系的特异性条带有5条,而普通品系的特异性条带有7条,这些特异标记可以用来鉴定滩羊的两个品系;滩羊体大品系与普通品系间的遗传距离为0.136±0.087,表明两品系之间的亲缘关系很近。     

不同生态型麦冬特异分子标记的选择与鉴定

采用RAPD技术对四川、湖北、浙江及上海等地 9个不同生态型的麦冬进行遗传多样性研究 ,筛选出 8个可以扩增出清晰、稳定、重复性好和多态性高的随机引物 ,通过对 8个有效引物的指纹图谱分析 ,发现 5个特异的RAPD分子标记 ,可以对 5个不同生态型的麦冬进行区别鉴定     

鲤亚目几种名优鱼的随机扩增多态性DNA分析

采用OPA、OPC和OPM三组共60条随机引物对鲤亚目的七种名优鱼和一种杂交品种进行了随机扩增多态性DNA(m)分析,对其中35个引物的扩增条带进行了统计,计算了它们之间的遗传相似率和遗传距离,在此基础上,利用UPGMA聚类分析建立了它们之间的亲缘关系,结果与传统分类是一致的,并且将杂交品种与纯种区分开来,实验还初步筛选了7种鱼的RAPD分子标记,探讨了用R6PD技术来鉴定名优鱼应用的可行性。     

废水处理系统中功能菌株特异分子标记

用40条10碱基随机引物对油脂化工废水处理系统中分离到的降解脂肪酸和脂肪胺功能菌株FA-2。FA-3及对照菌株进行RAPD分析。筛选出长度为700bp的FA-2的特异性片段,并克隆到pMD 18-T载体上．根据测序结果,设计了1对SCAR(sequence characterized anlplfied region)引物,通过SCAR-PCR反应,获得SCAR标记,并通过对20个培养样品进行PCR扩增,验证了标记特异性．结果表明该标记可作为特异的分子标记用于污水处理系统中功能菌株FA-2的监测．