第九届中国大学生健美操、艺术体操锦标赛艺术体操赛场情况分析

通过对第九届中国大学生健美操、艺术体操锦标赛艺术体操赛场情况的分析，得出以下结论：运动员的难度发展水平不够全面；成套动作的编排在满足规程要求的情况下，难度数量及价值的高低不决定成绩的高低；完成情况是取胜的关键因素。     

安庆市实施液化气混空气供气有关问题的探讨

通过分析安庆市在实施液化气混空气供气条件的基础上,讨论了相关的实施设备、技术问题等.     

关于问题A~mX=XAX=X更广泛性的研究

要对高等代数中的某个问题:A是一个n×n(n∈N)的正定矩阵,X是一个n×1的向量,X>0,m∈N则AmX=XAX=X进行更广泛的研究。     

高炉铁水含硅量预测的计算机在线实现

本文给出了铁水含硅量预测模型的三种在线辨识的方法，讨论了数据库的建立和多种预测模型的计算机在线实现。     

降水预测的模糊权马尔可夫模型及应用

中长期降水量的预测是气象科学的一个难点问题，论文首先基于降水过程存在大量不确定性、不精确性的特点，应用模糊有序聚类的方法建立降水丰桔状况的分级标准；然后针对降水量为相依随机变量的特点，采取以规范化的各阶自相关系数为权重，用加权的马尔可夫链来预测未来降水的丰桔变化状况；在此基础上，根据模糊集理论中的级别特征值，计算出具体的降水量；最后以山西省河曲水文站近50年的降水资料为实例，对该方法进行了具体的应用，收到了较为满意的结果，从而为提高中长期降水量预报的精度提供了一条值得探索的途径。     

一种基于色料混合理论的配色仿真算法

以工业配色应用为背景，首先探讨了计算机配色的两种基本理论：三刺激值理论与色料混合理论，然后分析了基于色料混合理论的织物染色算法的不足，提出了一种新的配色仿真算法，并应用于涂料配色实践。从仿真结果来看，该配色仿真算法计算精确，能够有效地减小配方色差。     

异羟肟酸比色法测定电解混合液中葡萄糖酸的含量

本文报导用异羟肟酸比色法,分析葡萄糖电解氧化制备葡萄糖酸盐过程的电解混合液中葡萄糖酸根含量的研究结果,文中对实验条件及影响因素进行了探讨。实验证明,本方法具有分析方法简单、分析数据稳定、重现性好、可靠、方便等特点。     

Isolation of human epidermal stem cells by adherence and the reconstruction of skin equivalents

The isolation of human epidermal stem cells is critical for their clinical applications. In the present study, we isolated three populations of epidermal keratinocytes according to their ability to adhere to collagen type IV: i.e., rapidly adhering (RA), slowly adhering (SA), and non-adhering (NA) cells. The aim of this study was to characterize RA cells and to investigate the possibility of using these cells for epidermis reconstruction. To identify RA cells, flow cytometric analysis was performed using anti-₆ integrin and anti-CD71 antibodies. RA cells express high levels of ₆ integrin and low levels of CD71, which are considered as markers of an epidermal stem cell nature. Furthermore, electron microscopy showed that RA cells are small and have a high nuclear to cytoplasmic ratio, whereas SA and NA cells have well-developed cellular organelles and abundant tonofilaments. Western blot analysis showed that RA cells are slow cycling and express p63, a putative epidermal stem cell marker, whereas SA and NA cells express c-Myc, which is known to regulate stem cell fate. To compare epidermal regenerative abilities, skin equivalents (SEs) were made using RA, SA, and NA cells. The epidermis constructed from RA cells was well formed compared to those formed from SA or NA cells. In addition, only SEs with RA cells expressed ₆ integrin and ₁ integrin at the basal layer. These results indicate that RA cells represent epidermal stem cells and are predominately comprised of stem cells. Therefore, the isolation of RA cells using a simple technique offers a potential route to their clinical application, because they are easily isolated and provide a high yield of epidermal stem cells.Received 2 July 2004; received after revision 20 August 2004; accepted 10 September 2004     

Caffeine as a psychomotor stimulant: mechanism of action

The popularity of caffeine as a psychoactive drug is due to its stimulant properties, which depend on its ability to reduce adenosine transmission in the brain. Adenosine A₁ and A_2A receptors are expressed in the basal ganglia, a group of structures involved in various aspects of motor control. Caffeine acts as an antagonist to both types of receptors. Increasing evidence indicates that the psychomotor stimulant effect of caffeine is generated by affecting a particular group of projection neurons located in the striatum, the main receiving area of the basal ganglia. These cells express high levels of adenosine A_2A receptors, which are involved in various intracellular processes, including the expression of immediate early genes and regulation of the dopamine- and cyclic AMP-regulated 32-kDa phosphoprotein DARPP-32. The present review focuses on the effects of caffeine on striatal signal transduction and on their involvement in caffeine-mediated motor stimulation.Received 8 July 2003; received after revision 7 September 2003; accepted 6 October 2003     

Horizontal gene transfer from Eukarya to Bacteria and domain shuffling: the α-amylase model

-Amylases are present in all kingdoms of the living world. Despite strong conservation of the tertiary structure, only a few amino acids are conserved in interkingdom comparisons. Animal -amylases are characterized by several typical motifs and biochemical properties. A few cases of such -amylases have been previously reported in some eubacterial species. We screened the bacterial genomes available in the sequence databases for new occurrences of animal-like -amylases. Three novel cases were found, which belong to unrelated bacterial phyla: Chloroflexus aurantiacus, Microbulbifer degradans, and Thermobifida fusca. All the animal-like -amylases in Bacteria probably result from repeated horizontal gene transfer from animals. The M. degradans genome also contains bacterial-type and plant-type -amylases in addition to the animal-type one. Thus, this species exhibits -amylases of animal, plant, and bacterial origins. Moreover, the similarities in the extra C-terminal domains (different from both the -amylase domain C and the starch-binding domain), when present, also suggest interkingdom as well as intragenomic shuffling.Received 17 October 2003; accepted 6 November 2003