pH敏感荧光探剂BCECF

2′,7′-二(羧乙基)-5(6)-羧基荧光黄(BCECF)是一种新型的pH荧光探剂,它在440nm、490nm两处的荧光强度的比值(PIR)与pH值相关。体外实验证明,pH值6.20～7.80范围内,FIR与pH呈线形关系。用BCECF标记的蟾蜍膀胱上皮细胞,在pH值6.30～7.70范围内,细胞内pH(pHi)与FIR呈线性关系。用该法首次测得蟾蜍膀胱上皮细胞phi=7.21(n=86)。     

三聚磷酸钠对草酸钙晶体生长的影响及其防石机理

采用扫描电子显微镜(SEM)和X-射线衍射(XRD)方法研究了三聚磷酸钠对草酸钙(CaOxa)晶体生长的影响.三聚磷酸钠能抑制一水草酸钙(COM)晶体的生长,同时诱导二水草酸钙(COD)晶体的形成.随着三聚磷酸钠浓度的增加,COM晶体的表面积减少.上述结果表明三聚磷酸钠是抑制CaOxa结石的一种潜在药物.     

2型糖尿病肾病患者红细胞膜上钠-钾ATP酶活性的变化

为了解2型糖尿病肾病(diabetic nephropathy,DN)不同时期红细胞膜上Na+-K+-ATP酶活性变化,为临床诊断、治疗提供理论依据,按尿白蛋白排泄率(urinary albumin excretion rate,UAER)将已确诊为2型糖尿病的51例患者分为3组糖尿病正常白蛋白尿组(DM1组),微量白蛋白尿组(DM2组),大量白蛋白尿组(DM3组);20例健康人作为对照组,男性11例,女性9例,平均年龄58.27±10.89岁.将对照组及实验组抽血做红细胞膜Na+-K+-ATP酶活性检测.结果表明DN各组红细胞膜Na+-K+-ATP酶活性较对照组均显著降低(均为P=0.000).说明DN患者红细胞膜Na+-K+-ATP酶活性下降,控制血糖、维持Na+-K+-ATP酶活性对改善DN预后有十分重要的意义.     

热敏灸治疗肛周脓肿术后尿潴留的临床观察

目的观察热敏灸治疗肛周脓肿术后尿潴留的临床效果。方法选择2016年6月~2017年6月就诊于我院的40例肛周脓肿术后尿潴留患者为研究对象,随机分为对照组和观察组各20例,对照组给予膀胱区按摩,水流声诱导及下腹部热敷治疗,观察组在此基础上给予热敏灸治疗。治疗后观察患者自行排尿时间、排尿完全通畅时间、膀胱残留尿量进行统计,计算临床治疗的有效率。结果经治疗后与对照组相比,对照组有效率为70%,观察组有效率为95%,观察组患者的临床总有效率明显优于对照组(P0.05)。结论热敏灸能有效改善肛周脓肿术后患者的尿潴留症状,利于患者排尿、缩短病程,疗效确切,值得临床推广应用。     

Two phorbol ester receptor affinities in partially transformed human urothelial cells and decrease of receptor binding in desensitized cells

The presence of specific binding sites for phorbol esters was studied in a transformed but non-tumorigenic human urothelial cell line HCV-29 by assay of specific binding of³H-phorbol-12,13-dibutyrate (³H-PDBu) to intact living cells.³H-PDBu bound specifically to HCV-29 cells in a saturable and competitive manner. Scatchard plot analysis of specific binding yielded a curved plot consistent with two binding sites with K_d of 11 nM and 102 nM, respectively. At saturation the corresponding PDBu binding capacities (B_max) were 8.8 pmol/10⁶ cells (5.2×10⁶ molecules bound per cell) and 2.8 pmol/10⁶ cells (1.7×10⁶ molecules bound per cell).³H-PDBu binding was displaced by biologically active phorbol ester tumor promoters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and mezerein,but not by tumor promoters such as L-tryptophan, anthranilic acid and sodium saccharin. In cells desensitized by pretreatment with 1 g/ml (2M) TPA or PDBu for 24 h the level of binding was reduced to 28% of the level in non-exposed cells. The ability of desensitized cells to bind³H-PDBu was gradually restored within 5–6 days. At the same time the cells became sensitive to the morphological alteration induced by PDBu. This suggests that desensitization of HCV-29 cells is due to a decreased receptor-ligand binding capacity probably associated with down regulation of the phorbol ester receptors.     

琼脂凝胶双向扩散法模拟尿石形成及影响因素的研究

采用双扩散法研究了琼脂凝胶介质的尿酸体系中环状及分形结构的产生,并考察了影响环生长的有关因素.结果表明:金属离子的种类影响环的个数;离子的浓度影响环的宽度;葡萄糖可调控Liesegang环的形成;而1,2-双(邻氨基苯氧基)乙烷-N,N-N',N'-四乙酸(2-BAPTA)则对环的形成有明显的抑制作用.     

Potentiating effect of aldosterone on the diuretic action of atrial extract

Summary The typical stimulatory effect of a rat heart atrial extract on urinary water, sodium, potassium and kallikrein excretion is significantly increased by a previous administration of aldosterone (0.5 g/100 g b. wt) in the rat.     

体外冲击波治疗复杂性上尿路结石

本文报告采用 ESWL 治疗复杂性上尿路结石57例的结果,全部碎石成功,无严重并发症发生。笔者在讨论中对采用体外冲击波碎石术治疗复杂性上尿路结石进行评价,并着重讨论其碎石治疗的原则以及输尿管石街的处理。     

平台石墨炉原子吸收法直接测定尿中铬

探讨了应用石墨炉原子吸收法测定尿中铬的方法。采用氯化钯作基体改进剂 ,有效地消除了尿基体对测定的干扰。本法检测限 0 .72 μg/L ,标准曲线线性范围 0 - 12 0μg/L,平均回收率 10 2 % ,精密度 5.75% ,方法指标符合“生物材料分析方法的研制准则”[1 ]     

Dynamics of excretion of urinary chemosignals in the house mouse (Mus musclus) during the natural estrous cycle

Summary The volatile fraction of urinary metabolites was investigated chromatographically at five different stages of the natural estrous cycle. A very substantial endocrine dependency has been noted for 11 compounds: 4 ketones, 2 acetate esters, 3 dihydrofuran isomers, dehydro-exo-brevicomin, and 2,5-dimethylpyrazine. The compounds were structurally verified through combined gas chromatography/mass spectrometry.