聚丙烯酰胺凝胶电泳体系中蛋白质的荧光标记分析

在室温下,采用聚丙烯酰胺凝胶电泳法,分离经荧光胺或对氯荧光胺荧光标记的蛋白质样品,可在2.5小时内完成蛋白质样品的分离和其区带的鉴定。耗时是传统方法的四分之一,而且荧光图象清晰、灵敏度较高。     

Separation of hydrophobic gold nanoparticles less than 10 nm by electrophoresis

An electrophoresis device was used to separate hydrophobic gold nanoparticles with particle size less than 10 nm dispersed in organic solvent. Image of transmission electron microscope （TEM） and UV-Vis spectra showed that the particles in the vicinity of cathode have a smaller mean diameter than those near the anode. This result indicates that the nanogolds in chloroform bring a positive charge in opposition to what is predicted by Coehn rule, and provides an electrophrosis method for separating hydrophobic nanogold particles of different sizes.     

基于在线小波变换的毛细管电泳信号滤噪方法

将在线小波变换用于毛细管电泳信号的滤噪处理,满足了毛细管电泳信号采集过程中实时处理和监控的需求.针对毛细管电泳信号的特点,采用固定时间窗的在线小波变换,并给出最佳时间窗长的选择方法.模拟信号和冬虫夏草的毛细管电泳信号滤噪实验的结果表明,该方法在保持峰位与峰高不变的情况下可显著提高信噪比,有效滤除了噪声峰.     

Elastic wave scattering by two-dimensional periodical array of cylinders

We extend the multiple-scattering theory (MST) for elastic wave scattering and propagating in two-dimensional composite. The formalism for the band structure calculation is presented by taking into account the full vector character of the elastic wave. As a demonstration of application of the formalism we calculate the band structure of elastic wave propagating in a two-dimensional periodic arrangement of cylinders. The results manifest that the MST shows great promise in complementing the plane-wave (PW) approach for the study of elastic wave. Foundation item: Supported by the National Natural Science Foundation of China (No. 10174054) Biography: Mei Jun (1977-), male, Master candidate, research direction: the multiple scattering theory     

Fe（OH）3胶体电泳实验再探索

在琼脂中加入适量的KNO3，经处理制成一定长度的琼脂柱，在其中插入铜线便制成Fe(OH)3胶体电泳实验的电极，探索了电压，电极间距，胶体浓度及环境温度等对实验效果的影响，提出了最佳的实验条件，获得了较快的电泳速度和清晰的电泳界面。     

二维自适应增量调制的研究

本文提出了一种新的数字通信方式——二维自适应增量调制(2-D ADM).通过研究、设计,井具体计算均衡的颗粒噪声功率,证明它是一种行之有效的、可靠的通信方式.     

随机变幅加载下疲劳强度可靠性分析

目前的疲劳强度及其概率分布是根据等幅Ｐ－Ｓ－Ｎ曲线族求得的，只能用于等幅加载，不能用于变幅及随机加载，而等效应力法又是根据常规ＭＩＮＥＲ准则进行转换的，无法用于可靠性分析，本文首次明确提出等幅疲劳强度，变幅疲劳强度及随机加载疲劳强度的概念，并且运用二维概率ＭＩＮＥＲ法则，通过建立变幅及随机加载的Ｐ－Ｓａ－Ｓｍ－Ｎｖ曲面族方程，提出了变幅及随机加载下疲劳强度可靠性分析的一套系统方法，具有重要的工程尖     

A proteomic analysis of bacterial strain<Emphasis Type="Italic">Sinorhizobium fredii</Emphasis> RT19 subjected to salt shock

Sinorhizobium fredii RT19, a strain of freeliving bacteria, was subjected to salt shock and its protein expression profiles were analyzed by differential display proteome approaches. The results of separation by two-dimensional polyacrylamide gel electrophoresis (2D PAGE) showed that the number of resolved proteins was 481, 465 and 424, corresponding to salt-free control, 5 and 50 min 1 mol/L salt treatment, respectively. Among the resolved proteins, 82 in total had altered expression in response to salt-shock stress. 26 out of the 82 proteins were induced and 23 were completely inhibited, while 12 were up-regulated and 21 down-regulated in response to salt shock. In addition, the appearance of differentially displayed proteins responding to different salt shock periods is also reported. The identity of the 26 induced proteins was revealed by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) followed by database searching. Among them, 20 were assigned to proteins with known functions. Their roles in response to salt shock stress are discussed.