宗教渗透特征分析

宗教渗透是境外敌对势力"和平演变"战略的重要组成部分,旨在破坏祖国统一,颠覆我国社会主义制度.宗教渗透具有六方面显著特征:政治恶意性、文化侵略性、活动国际性、手段多样性、组织秘密性、破坏严重性.认清这些特征有助于实际工作部门正确合理地处理宗教渗透问题.     

移植法建立大鼠肝癌模型

用肿瘤肝脏移植的方法建立了大鼠肝癌模型，对该模型的特点及应用价值进行了探讨，从为该模型简单，实用，适合于基础及临床研究。     

Matrix metalloproteinase 19 processes the laminin 5 gamma 2 chain and induces epithelial cell migration

In this study we analyzed the proteolytic activity of MMP-19 and its impact on keratinocyte migration. In the HaCaT keratinocyte cell line overexpressing wild-type MMP-19 (HaCaT-WT), transmigration through fibrin and type IV collagen matrices was significantly increased compared to cells harboring a catalytically inactive mutant (HaCaT-EA). Studying the expression of MMP-19 in early stages of squamous cell cancer (SCC), we found co-localization of MMP-19 and laminin 5 at the invading tumor front but not in suprabasal epidermis of the tumor. Examination of laminin 5 processing revealed increased processing of the 2 chain in the medium and matrix of HaCaT-WT cells and degradation by recombinant human MMP-19 to 105-kDa and 80-kDa fragments. Parental HaCaT grown on the matrix of HaCaT-WT and HaCaT-EA cells displayed differential tyrosine phosphorylation. Using integrin blocking and stimulating antibodies we could attribute these differences to a shift from 4-integrin-dependent signaling on the HaCaT-EA matrix toward 3-integrin-dependent signaling on the HaCaT-WT matrix. As a consequence, parental HaCaT showed increased migration on the matrix of HaCaT-WT cells. These data suggest that the MMP-19-dependent processing of the 2 chains leads to the integrin switch favoring epithelial migration and that MMP-19 actively participates in the early stages of SCC invasion.Received 29 October 2004; received after revision 7 December 2004; accepted 17 January 2005     

Myosin regulation in the migration of tumor cells and leukocytes within a three-dimensional collagen matrix

The migration of cells is a complex regulatory process which results in the generation of motor forces through the reorganization of the cytoskeleton. Here we present a comparative study of the expression and involvement of myosin in the regulation of the physiological migration of leukocytes and the pathological migration of tumor cells. We show that the involvement of myosin in the migration is distinct in these two cell types. In leukocytes, the activity of non-muscle myosin II is essential for both the spontaneous (matrix-induced) migration and the migration induced by ligands to G protein-coupled receptors, i.e. chemokines and neurotransmitters. In contrast, spontaneous tumor cell migration is largely independent of non-muscle myosin II activity, whereas the norepinephrine-induced migration is completely inhibited by either direct inhibition of non-muscle myosin II or of the kinases phosphorylating the myosin light chain, namely ROCK or the calcium/calmodulin-dependent myosin light-chain kinase.Received 31 August 2004; accepted 26 October 2004     

一个肿瘤侵入模型的定性分析

本文研究由Gatenby和Gawlinski在CancerResearch（Vol．56，1996）上提出的一个肿瘤侵入模型。该模型是一个强耦合的退缩型反应扩散方程组．本文在为α12零，0≤α21〈1的情况下，对该模型进行严格的数学分析。所获结果包括两个方面：（1）建立解的整体存在性。主要应用了逼近方法，H．Amann关于一般拟线性方程和这类方程与常微分方程耦合而成的广义的抛物型方程组的存在性理论，并且结合积分估计来证明解的存在性。如何建立解的积分估计是这个问题的关键所在。（2）研究解的渐近性态。通过构造Lyapunov函数，我们证明时变解在时间趋于无穷时将趋于稳态解。     

接种S180肉瘤的小鼠肿瘤组织和器官中组蛋白与正常小鼠的比较研究

采用酸抽提法分别提取接种S180肉瘤的小鼠肿瘤组织以及肝、脾、肾等器官的组蛋白和正常小鼠相应组织和器官的组蛋白，并采用SDS-PAGE方法进行检测．结果表明，正常小鼠的肝、脾、肾等器官提取的组蛋白在检测中可以看到组蛋白H1的亚带，而接种了S180肉瘤的小鼠的肿瘤组织以及肝、脾、肾等器官的组蛋白在检测中基本无明显的条带．这说明发生在小鼠身上的S180肿瘤组织对小鼠的肝、脾、肾组织中的组蛋白产生一定影响．     

甲状腺肿瘤中突变型P~(16)蛋白和PCNA的表达及意义

应用免疫组化LSAB法对22例甲状腺癌及20例甲状腺腺瘤的突变型P~(16)蛋白表达研究,结果发现:P~(16)甲状腺癌和甲状腺腺瘤表达率分别为45%及10%,两者表达率有显著性差异(P0.05);甲状腺恶性肿瘤中乳头状癌及滤泡性腺癌P~(16)的表达率两者无显著性差异(P>0.05).提示突变型P~(16)表达及PCNA的检测对研究癌基因在甲状腺癌发生发展中及细胞异型增生中有重要作用.     

Bromelain: biochemistry, pharmacology and medical use

Bromelain is a crude extract from the pineapple that contains, among other components, various closely related proteinases, demonstrating, in vitro and in vivo, antiedematous, antiinflammatory, antithrombotic and fibrinolytic activities. The active factors involved are biochemically characterized only in part. Due to its efficacy after oral administration, its safety and lack of undesired side effects, bromelain has earned growing acceptance and compliance among patients as a phytotherapeutical drug. A wide range of therapeutic benefits has been claimed for bromelain, such as reversible inhibition of platelet aggregation, angina pectoris, bronchitis, sinusitis, surgical traumas, thrombophlebitis, pyelonephritis and enhanced absorption of drugs, particularly of antibiotics. Biochemical experiments indicate that these pharmacological properties depend on the proteolytic activity only partly, suggesting the presence of nonprotein factors in bromelain. Recent results from preclinical and pharmacological studies recommend bromelain as an orally given drug for complementary tumor therapy: bromelain acts as an immunomodulator by raising the impaired immunocytotoxicity of monocytes against tumor cells from patients and by inducing the production of distinct cytokines such as tumor necrosis factor-α, interleukin (Il)-1β, Il-6, and Il-8. In a recent clinical study with mammary tumor patients, these findings could be partially confirmed. Especially promising are reports on animal experiments claiming an antimetastatic efficacy and inhibition of metastasis-associated platelet aggregation as well as inhibition of growth and invasiveness of tumor cells. Apparently, the antiinvasive activity does not depend on the proteolytic activity. This is also true for bromelain effects on the modulation of immune functions, its potential to eliminate burn debris and to accelerate wound healing. Whether bromelain will gain wide acceptance as a drug that inhibits platelet aggregation, is antimetastatic and facilitates skin debridement, among other indications, will be determined by further clinical trials. The claim that bromelain cannot be effective after oral administration is definitely refuted at this time. Received 25 August 2000; received after revision 29 March 2001; accepted 30 March 2001     

MVD及VEGF表达与鼻咽癌侵袭转移关系的研究

为探讨微血管密度（ＭＶＤ）及血管内皮生长因子（ＶＥＧＦ）的表达与鼻咽癌（ＮＰＣ）侵袭转移关系,在分子水平干预肿瘤血管生成,预防ＮＰＣ复发和转移打下基础,采用免疫组织化学Ｓ－Ｐ法检测了７３例ＮＰＣ,１５例鼻咽良性肿瘤、２０例无瘤鼻咽部石蜡标本组织中的ＭＶＤ及ＶＥＧＦ表达,ＮＰＣ中转移组４９例,非转移组２４例。     

Genistein and Daidzein Effects on Proliferation, Cell Membranes, Cell Cycles and Cell Apoptosis of Different Cell Lines

IntroductionIsoflavonoids are found mainly in soybeans,aprimary food source for humans and herbivores.The structure and function of isoflavonoids aresimilar to 1 7β- estradiol (1 7β- E2 ) ,and have dualeffects as both mammal oestrogenicity and anti-oestrogenicity,so they are also termed isoflavonicphytoestrogens(IPE) .It has been reported thatthese compounds can reduce blood fat,as well asthe risk of cancer and osteoporosis[1] .Genistein and daidzein are two principleisoflavonoids in soybe…