针吸细胞学检查在乳腺癌诊断中的意义

目的：观察乳腺肿物针吸细胞学诊断与组织病理学检查的符合率。方法：对166例乳腺肿物吸出组织，经巴氏（Papanicolaou)染色，参照分级标准及良、恶性肿瘤组织细胞的形态进行了显微镜下早期诊断。结果：166例针吸细胞学诊断与组织病理检查诊断符合率为89.76%、灵敏度84．81％、特异度94．25％。其中良性肿瘤69例，假阴性12例（12．77％）；乳腺癌和高度怀疑有癌特征的97例，假阳性5例（6.94%)。结论：乳腺针吸细胞学检查在乳腺肿物的临床诊断中具有准确、可靠、安全、经济等重点意义，可以作为乳腺癌诊断的重要手段。     

海马生髓丸对S180荷瘤鼠、SKOV3卵巢癌模型鼠抑瘤机理的实验研究

以S180荷瘤鼠、SKOV3卵巢癌模型鼠为研究对象,进行海马生髓丸抗肿瘤的实验研究.通过对移破植瘤的抑瘤率、光、电镜技术及细胞凋亡调控基因P53、BcL-2等免疫组化的研究,表明海马生髓丸具有一定的抗肿瘤作用,可以抑制S180荷瘤鼠的肿瘤生长,明显增强SKOV3卵巢癌模型鼠DDP的杀伤肿瘤作用,对肿瘤细胞的凋亡具有明显促进作用.     

论吴氏抗金在南宋军事史上的地位

吴氏在西北的抗金活动 ,挫败了金人入蜀的图谋 ,消灭了敌人有生力量 ,牵制了金人南下进攻南宋的行动 ,为巩固南宋政权发挥了重要的作用。吴、吴善打硬战 ,在与金人交锋中创造出一系列著名的战例。他们熟悉战争规律 ,根据敌我双方的特点 ,采取灵活多变和充分利用有利地形的战术 ,积累了宝贵的经验 ,极大地丰富了中国军事思想的宝库。他们以抗击侵略为己任 ,不卷入政治斗争 ,不争战功 ,不炫耀战绩 ,具有古代军人可贵的精神风范。他们的抗金活动甚至在整个中国古代战争史上都占有重要的地位     

MRI与CT对恶性胸膜间皮瘤的诊断价值比较

目的：探讨、比较MRI和CT在恶性胸膜间皮瘤临床诊断中的意义。方法：25例经病理组织学或细胞学证实的恶性胸膜间皮瘤患者，术前均进行了MRI和CT检查；比较分析其影像学表现特点及其临床意义。结果：MBI除了对肿瘤内钙化的显示率低于CT外，对恶性胸膜间皮瘤形态学的观察普遍优于或等于CT，尤其对叶间胸膜及横膈膜增厚的显示、肿块侵犯胸壁或膈肌以及肺门或纵隔淋巴结构的显示优于CT。恶性胸膜间皮瘤在T1WI上呈低信号或等信号，而在T2WI及增强T1WI上绝大部分呈高信号。结论：与CT比较，MRI对恶性胸膜间皮瘤的TNM分期以及治疗方案的制订更具指导意义。     

Strategy against micrometastasis of epithelial cancer: Detection and elimination

Tumor metastasis is generally agreed to be the major cause of cancer death. Over the last few years, studies of new diagnosis techniques and tumor immunotherapy have made great progress. Recent clinical studies on the occult metastases of breast, lung and colorectal cancer all suggested that the detection of micrometastases in bone marrow is prognostically important and provides substantial evidence of tumor dissemination. On the other hand, two kinds of the mAb-based immunotherapy have been approved for the treatment against epithelial cancer. Monoclonal antibody (mAb) 17-1A for colorectal carcinomas and mAb herceptin for breast cancer both have produced good curative effects. Potential therapeutics based on some antibodies with prominent antitumor activity also has shown obvious clinical effect. These studies indicate that detection of micrometastasis in circulatory system and immunotherapy by eliminating metastatic malignant cells suggested a new strategy against the metastatic cancer.     

Molecular Cloning of a Novel Bovine Homologue of the Drosophila Tumor Suppressor Gene,Lats

Pervious studies demonstrate that lats,also known as warts,is a tumor suppressor gene in Drosophila^[1,2],Mutations of lats lead to an increase in cell number and organ size in Drosophila,indicating lats may be involved in organ size control.Furthermore,the high conservation of sequence and tumor suppression functioin of lats between Drosophila and human suggests that it may be also involved in organ size control of higher animals^[3],So here we isolated the bovine homologue of Drosophila lats.Sequence analysis indicates the bovine LATS1 to be very similar to other lats proteins.     

Interaction of hepatitis B virus with tumor suppressor gene p53: its significance and biological function

The mechanism of the interaction of hepatitis B virus (HBV) with tumor suppressor p53 and its role in the hepatocar-cinogenesis have been studied by PCR-directed sequencing, gel shift assays and in situ ultraviolet cross-linking assay. The biological function of the interaction of HBV with p53 gene was investigated by co-transfection of chloramphenicol acetyltransferase ( CAT) reporter gene. p53 and HBV DNA. and quantitative PCR. Among the 16 primary hepatocellular carcinoma (PHC) samples. 13 were HBV-DNA positive. 10 HBxAg positive and 9 p53 protein positive. The p53 gene point mutation was found in 5 samples, one of which had a G to T substitution located at codon 249. After analyzing the HBV genome by a computer program, a p53 response element binding sequence was found in HBV genome at upstream of enhancer I. from 1047 to 1059 nucleotides. This sequence could specifically bind to p53 protein, increase p53 protein accumulation in the PHC cells and stimulate the transactivating activity of p53 and HBV replication . The results also revealed that HBxAg could combine with p53 protein to form a complex in the cells and enhance CAT expression. Immunocytochemical staining showed that p53 protein complex was located in the cytoplasm and the process of p53 entry to nuclei was. in part, blocked. From our results, we conclude that the mutation of p53 gene at codon 249 is infrequent in HBV-associated PHC. the DNA-protein binding between HBV and p53. and the protein-protein binding between HBxAg and p53 might lead to the reduction or inactivation of p53 protein, which in turn resulting in HBV-associated hepatocarcinogenesis.     

Electroporation： A New Approach Enhancing Antitumor Effects of Cytoxan

Electrochemotherapy (ECT) is a novel cancer treatment in which electric pulses (EPs) inducing cell membrane pored (eleetroporation) are used as a means of delivering antitumor drugs to the cytoplasm of cancer cells. In vitro, with scan electromicroscope (SEM) and Trypan blue staining examination, the best parameter of EPs of electroporation is studied by the S-180 cells exposed to EP with various voltages, pulses, capacitance. The best parameter of EP of electroporation is 600V/cm, 6 pulses, 10 μF. In the in vivo study, ECT is studied with the Cytoxan (CTX) injected directly into the tumor followed immediately by a local EP at the tumor site.Four parameters, which include the tumor inhibitory ratio, the curing ratio and the vas capillareof tumor, the tumor‘s histopathological characteristics are determined and compared among the ECT group, the control group, the EP-only group and the drug-only group. The results indicate that the antitumor effect of CTX is sitrnifiezntlt enhanced by electroporation.     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system on the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the sensitivity of chemotherapy and radiotherapy. E1A have the ability to integrate into the host genome, resulting in long-time expression that induces Rb gene inactivation and animal cells immortalization. This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy. Thus, we firstly constructed E1A eucaryotic expression vector (pPIC9/E1A), transformated the pichia pastoris yeast cells (GS115) and screened the high-expressing recombinant strains. The positive yeast strains were cultured in the shake flask, and induced for 3 d. The crude E1A protein was purified using two steps of column chromatography on HiTrap Q and HiTrap SP. The purified E1A protein was identified by SDS-PAGE and Western blot. E1A protein was mostly located at cellular nuclear when Chariot delivered E1A protein into cells. The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase, and significantly inhibited the growth of LN686 tumor cells. The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

稀土丙二酸类配合物的合成和体外抗癌活性的初步研究

合成了七个稀土丙二酸类配合物(RE2(C3H2O4)3,RE=Sm,Eu,Gd,Dy,Nd)、1,1-环丁烷二羧酸铕(Eu2(C6H6O4)3)和1,1-环戊烷二羧酸铕(Eu2(C2H8O4)3),其中1,1-环丁烷二羧酸铕和1,1-环戊烷二羧酸铕稀土配合物为首次报导。经元素分析、红外光谱、热分析确证了它们的组成为RE2A3·nH2O(n=6,3,2)。体外抗肿瘤活性实验表明有一定的细胞毒活性。