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131.
DNA甲基化已成为阐明正常和病理基因表达现象的重要机制,因而已成为当前分子生物学的研究热点之一。对DNA甲基化的转录抑制机制、在肿瘤发生中的作用、与细胞衰老和凋亡的关系、抑制剂以及分析方法等方面的研究新进展进行了综述。 相似文献
132.
为了研究倾斜煤层开采条件下的突水规律,在考虑原岩应力、地质构造、地下水、采动影响等因素基础上,从应力场和渗流场共同作用的角度出发,研究含底板岩体在内的采场岩体系统的变形与破坏。研究结果表明,随着工作面的推进,底板岩层的破坏深度和范围加速增加。研究结论将数值模拟结果与工程实际进行比较分析,为寻求安全、经济的开采方法提供了理论依据。 相似文献
133.
Shen Xianrong Ji Dongmei Hu Youqing Jia Fuxing Deng Xinxian Wang Ling Chu Zhiyong Jiang Dingwen Lei Chengxiang Huang Jiansheng Hu Xinran Ren Daming 《科学通报(英文版)》2001,46(24):2037-2042
Sequences encoding PF4 (58–70) and TSP1 (429–459) were linked to yield a single gene TSF which encodes the fuse-protein of TSF. The gene was cloned into a pGEX-2T expression vector to generate a protein GST-TSF, which was strongly expressed inE. coli. The purified GST-TSF was degraded with thrombin to generate the protein TSF. With the methods of MTT and wound repair assay, the effects of TSF on the proliferation and migration of EC were detected, respectively. The results showed that TSF significantly suppressed BAEC proliferation and migration in a dose-dependent manner. The fuse protein GST-TSF, and the peptides PF4 (58–70) and TSP1 (429–459) also inhibited BAEC proliferation and migration, respectively, but their inhibition rates were not as high as TSF. Using the CAM assay, it was shown that TSF, GST-TSF, PF4 (58–70) and TSP1 (429–459) inhibited angiogenesis in chick CAM potentially, the effect of TSF was the highest.In vivo, the growth of Lewis lung carcinoma was potently inhibited by TSF treatment, and the inhibition rate was 68.75% at a dose of 1.00 μmol/kg · d. These findings suggest that the design on TSF gene was successful, and TSF with its anti-angiogenic and anti-tumor activity, should be a useful source of the inhibitor. 相似文献
134.
DNA甲基化在肿瘤形成中的作用(综述) 总被引:2,自引:0,他引:2
DNA甲基化改变是肿瘤细胞中常见的现象,DNA甲基化与肿瘤的发生有密切关系。从以下几方面对此做一综述。(1)简介哺乳动物细胞的DNA甲基化;(2)DNA甲基化与肿瘤基因突变;(3)肿瘤DNA甲基化的基因外作用,其中包括:原癌基因的低甲基化和抑癌基因的高甲基化。 相似文献
135.
砷剂化合物作用机制的研究概况(综述) 总被引:1,自引:0,他引:1
砷剂对急性早幼粒细胞白血病有良好的疗效,研究发现砷剂的作用机制可能包括:(1)诱导细胞凋亡和部分分化;(2)调控细胞凋亡基因的表达;(3)使线粒体内膜跨膜电位(transmembrane potential,△ψm )下降;(4)阻抑细胞增殖。 相似文献
136.
HSP70在肺癌组织中表达水平的研究 总被引:3,自引:0,他引:3
采用免疫组织化学SABC法检测39例肺部组织中HSP70的表达。结果显示:HSP70在高度分化的肺鳞癌和肺腺癌细胞中的阳性表达率与癌旁正常肺组织之间具有高度显著性差异(P〈0.01),HSP70的表达在高分化的肺鳞癌与肺腺癌之间则无显著性差异(P〉0.05),在低分化的肺鳞癌和肺腺癌组织中,HSP70呈阴性反应,HSP70在肺癌组织中表达水平的高低与肺癌细胞的分化程度密切相关,而与肺癌的组织学类型 相似文献
137.
Jun Yang Ying Wu Ping Ma Yanhua Zhao Ying You Susan E. Kane Ning Mao Yangpei Zhang 《科学通报(英文版)》2000,45(15):1390-1394
A series of retro viral vectors encoding humanmdr1 gene alone as wetl as in combination with either humanmgmt gene or human mutantSer
31-dhfr gene are engineered. The resultant retroviruses are used to transduce human umbilical cord blood CD34+ cetls. It has been shown that expression of dual drug resistance genes in transduced cetls confers a broad range of resistance
to both kinds of corresponding drugs. These data suggest a rationale for the use of such double chemoresistance gene constructs
in anin vivo model in which transduced hematopoietic cetls will acquire multiple protection against the cytotoxic side effects of combination
chemotherapy and may have future application in chemoprotection of normal tissues, thus killing tumor cetls more effectivety. 相似文献
138.
139.
140.
Strell C Lang K Niggemann B Zaenker KS Entschladen F 《Cellular and molecular life sciences : CMLS》2007,64(24):3306-3316
The extravasation of leukocytes and tumor cells is a multi-step process with the involvement of various adhesion molecules
depending on the three steps rolling, adhesion, and diapedesis. We have developed an in vitro model, by which we investigated the rolling and adhesion of neutrophil granulocytes and MDA-MB-468 human breast carcinoma
cells to lung endothelial cells under physiological flow-conditions. We found that norepinephrine had an inhibitory function
on the fMLP-promoted adhesion of neutrophil granulocytes due to a down-regulation of β2-integrin. Furthermore, neutrophil
granulocytes serve as linking cells for the interaction of the MDA-MB-468 cells with the endothelium, which are both β2-integrin
negative, but express the β2-integrin ligand ICAM-1. In addition, we show here that N-cadherin is up-regulated on the endothelial
cells and on neutrophil granulocytes in response to fMLP. This up-regulation resulted in a significant increase of adherent
MDA-MB-468 cells, which are also N-cadherin positive.
Received 3 September 2007; received after revision 17 October 2007; accepted 22 October 2007 相似文献