拟南芥AtGluRS相互作用蛋白质VDAC及其转基因植物表型分析

电压依赖性阴离子通道蛋白质(voltage-dependent anion channel,VDAC)是拟南芥谷氨酰tRNA合成酶(AtGluRS)相互作用的蛋白质．为了研究VDAC蛋白的功能和作用,通过构建VDAC稳定表达载体,农杆菌介导的方法转化拟南芥,筛选和鉴定出VDAC过量和抑制表达植株．研究结果证实VDAC的过量和抑制表达植株影响气孔关闭和种子萌发．VDAC的过量表达导致植株对ABA敏感,VDAC的抑制表达降低了植株对ABA的敏感性．推测VDAC蛋白可能参与ABA信号途径．     

水分生产函数求解方法及比较

文章以玉米为研究对象分别基于Jensen模型和水分敏感指数积累函数模型求解水分敏感指数,并且对两种模型的精度进行比较。     

未来气候变化情景下我国粮食供需平衡风险研究

“粮食供需平衡风险”定义为未来气候变化对我国粮食供需的影响程度。基于IPCC 排放情景特别报告(SRES)中B2情景气候条件下的我国粮食生产模拟结果, 构建该框架下我国粮食消费分析情景, 最终建立以自给粮食供应程度为核心的粮食供需平衡风险标准。借助GIS空间分析技术, 探讨县域尺度上近期、中期和远期的气候变化将会给我国粮食供需平衡带来怎样的风险局面。结果表明气候变化将会在一定程度上加剧我国的粮食供需平衡形势。3个时段内, 粮食供需风险局面严峻形势由高到低排序: 中期>远期>近期。具体地, 中期面临的供需风险的县市比例高达66.99%, 其中中风险和高风险区域范围最广, 两者所占比例高达29.5%; 近期风险形势相对较为缓和, 上述比例降低到25.54%, 其中高风险县市比例较低约为10.72%, 中风险约为14.82%; 在空间格局上, 黄淮海区、黄土高原区、西南区以及青藏区的东缘面临的风险形势相对较为严峻, 东北区、甘新区以及内蒙及长城沿线区则相对较为良好。     

Ubi1 intron-mediated enhancement of the expression of Bt cry1Ah gene in transgenic maize (Zea mays L.)

The cry1Ah gene was one of novel insecticidal genes cloned from Bacillus thuringiensis isolate BT8. Two plant expression vectors containing cry1Ah gene were constructed. The first intron of maize ubiqutin1 gene was inserted between the maize Ubiquitin promoter and cry1Ah gene in one of the plant expressing vectors (pUUOAH). The two vectors were introduced into maize immature embryonic calli by microprojectile bombardment, and the reproductively plants were acquired. PCR and Southern blot analysis showed that foreign genes had been integrated into maize genome and inherited to the next generation stably. The ELISA assay to T1 and T2 generation plants showed that the expression of Cry1Ah protein in the construct containing the ubi1 intron (pUUOAH) was 20% higher than that of the intronless construct (pUOAH). Bioassay results showed that the transgenic maize harboring cry1Ah gene had high resistance to the Asian corn borers and the insecticidal activity of the transgenic maize containing the ubi1 intron was higher than that of the intronless construct. These results indicated that the maize ubi1 intron can enhance the expression of the Bt cry1Ah gene in transgenic maize efficiently     

害虫综合治理模型与最优控制策略

针对农作物害虫综合治理问题,提出一个对农作物危害最小的最优方案。由于喷洒杀虫剂,农作物被迫吸收农药,药效随时间下降,其吸收、分布和消除过程与人类用药类似,作者大胆尝试利用药物动力学理论研究建立农作物药效模型。首先根据脉冲微分方程理论,将农作物药效模型与害虫-天敌动态模型结合起来,建立农作物药效模型和喷洒杀虫剂及释放天敌的脉冲控制模型;然后根据脉冲控制理论分析上述模型的稳定性,利用最优控制理论,求出最适杀虫剂药量和喷洒时间间隔,使得杀虫剂药量在农作物的残留和喷洒农药量最少,同时使害虫数量控制在经济危害阈值以下,给出综合治理农业害虫的最佳方案;最后,通过数值模拟解释这一方案的执行。结果表明,该策略行之有效。     

转基因技术及其在水稻育种中的研究进展

以载体介导、基因直接导入和种质系统3类基因转化体系分别介绍了常用的几种转基因技术—农杆菌转化法、基因枪法、PEG介导法、电击法、花粉管通道法和浸泡转化法的原理及其在水稻育种上的最新研究进展,比较了各种技术的特点。最后对转基因技术在水稻育种上的前景作了展望。     

基于作物水胁迫声发射的精准灌溉模糊控制模型

设计了基于作物水胁迫声监测精准灌溉的模糊控制系统,论述了正常灌溉和精准灌溉两种不同工作方式下常规双模模糊控制策略的制定,控制策略的目标是既要保证作物正常生长又要实现节水效果.在此基础上,建立适用于作物生长环境的自学习模糊控制模型.实验表明:系统能够根据声发射号及影响作物生长的环境信息在一定范围内进行给水量的调节,实现作物的精准灌溉,避免正常灌溉时效率低下、施水过量的问题.     

Review and prospect of transgenic rice research

Rice is one of the most important crops as the staple food for more than half of the world＇s population. Rice improvement has achieved remarkable success in the past half-century, with the yield doubled in most parts of the world and even tripled in certain regions, which has contributed greatly to food security globally. Rapid population growth and economic development pose a constantly increased food requirement. However, rice yield has been hovering in the past decade, which is mainly caused by the absence of novel breeding technologies, reduction of genetic diversity of rice cultivars, and serious yield loss due to increasingly severe occurrences of insects, diseases, and abiotic stresses. To address these challenges, Chinese scientists proposed a novel rice breeding goal of developing Green Super Rice to improve rice varieties and realize the sustainable development of agriculture, by focusing on the following 5 classes of traits： insect and disease resistance, drought-tolerance, nutrient-use effi- ciency, quality and yield potential. As a modern breeding approach, transgenic strategy will play an important role in realizing the goal of Green Super Rice. Presently, many transgenic studies of rice have been conducted, and most of target traits are consistent with the goal of Green Super Rice. In this paper, we firstly review technical advances of rice transformation, and then outline the main progress in transgenic rice research with respect to the most important traits： insect and disease-resistance, drought-tolerance, nutrient-use efficiency, quality, yield potential and herbicide-tolerance. The prospects of developing transgenic rice are also discussed.     

Generation of transgenic cucumbers with expression of a ten-tandem repeat long-acting GLP-1 analogue and their biological function on diabetic rats

Glucagon-like peptide-1 （GLP-1） is a 30-amino acid peptide hormone, which has the regulatory function of stimulating the secretion of insulin to balance blood glucose levels. In this study, marker-free expression vector pX6 with a fusion gene of ten tandem repeated GLP-1 analog （[Ser8, Gln26 and Asp34]-GLP-1） genes was constructed and transformed into cucumbers by Agrobacterium tumefaciens-mediated transformation method. Four transgenic lines were obtained by PCR and Southern blotting analysis and two transgenic lines successfully expressed the fusion protein （named GLP-T）, which was revealed by Western blotting analysis. The biological activity test results showed that the serum glucose level of diabetic rats was significantly decreased after oral administration of the fusion protein GLP-T extracted from the transgenic cucumber fruitage. These results suggest that this may provide a brand-new strategy to prevent and cure the diabetes with no pain.     

现代生物技术在开发新食品中的应用研究

论述了基因工程"向日豆"的育出、运用固定化微生物和固定化酶改善食品风味、运用蛋白质工程改善食品品质的技术、甜味蛋功能基因在高等植物中获表达以及基因工程作物产出食用疫苗的研究.并对其未来发展进行了展望.