全国文化信息资源共享工程的重要服务模式——联合参考咨询与文献传递网——以哈尔滨市图书馆为例

联合参考咨询与文献传递网将全国优秀的文化信息资源进行数字化加工整合,通过共享工程网络体系,让文化信息资源在最大范围内传递和应用。以哈尔滨市图书馆在联合参考咨询与文献传递网服务中的数据为例,说明了联合参考咨询与文献传递网在共享工程中的显著作用。     

临汾市引沁入汾和川取水输水工程GPS施工控制网的布设与实施

介绍了临汾市引沁入汾和川取水输水工程,对GPS施工控制网的布设、实施野外观测及数据处理等方面进行了论述,为今后在施工控制网测量中采用GPS测量技术总结了一些有益经验。     

新型气相色谱分析样品前处理装置与方法

探索一种新型的样品前处理装置,运用载体、携带体和综合蒸馏技术完成了待测样品中微量成分的提取分离和富集.将冷端接受器设计为可更换结构,所以无样品交叉干扰,适用于痕量组分的富集.在乙酸乙酯中添加了4种微量有机磷农药后进行了前处理其装置能自动将微量组分富集到2mL邻苯二甲酸二甲酯中利用气相色谱分析的结果,研究了样品前处理装置分离富集参数的选择原则.实验证实该方法能最有效地除去蛋白、脂肪、色素等对气相色谱分析有干扰的组分;将待测微量组分的浓度富集150倍以上;回收率92%～117%;相对标准偏差±5.65%;最小检出线为1 ng/mL.有机试剂的回收利用率大于95%,而且操作简单方便,以模拟试样为例说明了方法的实用性.     

Cationic polymeric nanoformulation: Recent advances in material design for CRISPR/Cas9 gene therapy

CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/clustered regularly interspaced short palindromic repeat associated proteins 9) gene editing platform is a promising therapeutic tool for genetic disorders, due to its ability to manipulate the pathogenic gene in genomic level and to easily target specific gene by manipulating single-guide RNA. However, its successful delivery remains a challenge. Up to now, great efforts have been made to explore an effective strategy for CRISPR/Cas9 delivery. But among those delivery methods, physical methods are mainly operated on cultured cells thus limited to laboratorial use; viral vectors are hindered by fetal immunogenic and carcinogenic effects thus dubious in clinical application. Therefore, cationic polymeric vectors, with the ability to interact with CRISPR/Cas9 system to form a nanoformulation as a non-viral approach, are attracting increasing attentions, due to advantages such as well protection of cargos, less limitation in payload size, low immunogenicity or carcinogenicity, potential modifications for further functions, and ease in mass production. In this review, the recent discoveries on polymeric vectors utilized in delivery of CRISPR/Cas9 system will be summarized. With emphasis on advanced features of those polymeric vectors or their nanoformulations to meet the demands of different CRISPR/Cas9 delivery forms (plasmid, mRNA or protein), the detailed illustrations on their disease treatment applications, such as cancer, diabetes or antibiotic-resistant infections, will also be reviewed.     

Perspectives on the prevention and treatment of infection for orthopedic tissue engineering applications

The emergence of orthopedic tissue engineering devices as viable clinical solutions demands that the field address significant clinical complications associated with the implantation of foreign materials in the body, especially infection. While tissue engi- neering has focused on the development of methods to regenerate and repair, until recently there has been a relative dearth of literature regarding the intersection of tissue engineering and infection. In particular, local delivery of antimicrobials has long been of clinical interest, but only recently has that been translated into the realm of tissue engineering. In this perspective, we briefly review major modes of local delivery for infection prevention and treatment and discuss possible strategies for preventing implant-associated infections.     

高校文献传递工作浅议

阐述了文献传递工作面临的主要问题,通过文献传递工作整个工作流程的展示,说明了如何才能做好文献传递工作的每个环节,使文献传递工作得以顺利开展。     

NOVELL 网络上文件快速传输的实现

通过对ＮＯＶＥＬＬ网络传输协议ＩＰＸ的对等通信原理的分析，给出了ＮＯＶＥＬＬ网络中任意站点间文件快速传输技术的新的实现方法。     

黑龙江科技学院图书馆原文传递工作的实践与体会

从原文传递工作的实际出发，从如何做好用户宣传、用户培训及补贴政策3个方面进行了探讨。阐明了原文传递工作已成为信息服务的有效手段，必将在图书馆的服务整合中得到广泛地发展与普及。     

考虑了交箱时间不确定性的场桥堆存作业优化

客户送箱时间的不确定性直接影响出口箱的场桥堆存作业效率和其在集装箱码头堆场的堆存状态,进而增加场桥作业移动距离以及后续装船过程中的翻箱作业量,降低码头作业效率.针对该问题通过马尔可夫链预测方法将随机交箱序列转化为广义交箱序列,在此基础上,结合集装箱港口作业实际,建立了以最小化预翻箱数量和场桥堆存作业移动距离为目标的场桥调度优化两阶段数学模型,并分别针对初始决策阶段和实时调度阶段设计了静态与动态求解算法.通过仿真算例,借助于与不同堆存策略的对比,显示了优化模型及算法的实用性与有效性,为场桥调度方案的实时生成提供决策支持.     

建设管理模式匹配关系及其对项目绩效的影响

建设管理模式对项目内外部条件的适应程度直接影响项目绩效水平.基于对建设管理模式的研究成果和实践案例分析,揭示建设管理模式描述要素和项目条件要素之间的匹配关系对项目绩效的影响,构建了要素之间的匹配关系模型.提取世界银行在中国投资的30个公共建设项目案例数据,通过Spearman相关分析对模型作了定量检验,分析了匹配关系对项目绩效的影响及其作用机理.结果表明,在项目中咨询方通过项目可行性研究等前期工作促进了业主对项目的风险控制水平,从而提高了项目绩效,管理模式和项目条件之间的匹配关系越合理则项目绩效越良好.据此,提出了项目建设管理模式选择的建议和原则,建立的模型和成果对类似工程建设管理模式的构建具有借鉴意义.