Effects of butyrate and insulin and their interaction on the DNA synthesis of rumen epithelial cells in culture

Summary Rumen epithelial cells (REC) were incubated in the presence of various concentrations of butyrate or insulin or with both of them, to obtain information on their effect on the DNA synthesis of cultured cells. The 24-h values of³H-thymidine incorporation into cellular DNA were measured in the presence of butyrate, insulin or butyrate plus insulin. While butyrate reduced DNA synthesis, insulin produced an increase over the control. Combined butyrate plus insulin treatment influenced the incorporation of label in accordance with the relative proportion of these two substances.     

Urushiol components as mediators in DNA strand scission

Summary Poison oak urushiol, a mixture of 3-alk(en)ylcatechol derivatives was found to mediate DNA strand scission in the presence of oxygen and with copper(II) chloride as a catalyst. The reactionis believed to occur via activated reduced oxygen produced during oxidation of the catechol into itso-quinone derivative.     

菏泽地区持续开发利用黄河水资源研究

在分析黄河水资源开发利用现状和发展趋势的基础上，对黄河来水量进行了预测，并提出了菏泽地区扩大引黄的可行性和对策措施     

脱氧胞苷激酶基因的DNA探针的制备和纯化

本文报道乳酸杆菌脱氧胞苷激酶基因的DNA探针的人工合成和分离纯化以及杂交条件的探讨。结果表明:合成的探针可与酶解的核DNA杂交,显示出三条杂交带。     

能量集成改造的全局夹点分析法

考虑全局中各个过程之间以及与公用工程之间的相互影响，扩展夹点分析法于全局系统，提出了一种系统的全局能量集成改造方法。用该方法可进一步节省燃料或增加联产功，并应用实例证明了它的有效性。     

叶绿体DNA种内多样化

着重讨论了９科３２种植物叶绿体ＤＮＡ种内多样化，进而探讨叶绿体ＤＮＡ种内多样化的遗传学基础和意义。     

地高辛标记DNA探针应用于凝胶转移电泳

半抗原DIG-ddUTP与DNA连接,以此作为探针可以与其相识别的蛋白质相结合,再用带有碱性磷酸的抗体处理,形成的复合物可作用于底物CSPD,使其产生萤光,而被x光片记录下来。     

Approximation solvability of hot rolling problem with Coulumb friction

For hot rolling process, the property of gradient mapping of the plastic deformation power rate func- tional is discussed with a rigid-plastic slightly compressible material model. Using the existence and monotonicity of sub-differential of surface friction power rate functional as well as the nonlinear functional analysis methods, the approximation solvability of hot rolling problem with simplified Coulumb friction is obtained.     

Mitochondrial DNA variation in social wasps (Hymenoptera,Vespidae)

Summary Patterns of restriction fragment length polymorphisms (RFLP) of European Vespinae were more similar within genera than between them. Distance trees were constructed that support the hypothesis of monophyly of the generaVespula andDolichovespula. Within the genusVespula, V. germanica was more closely related toV. rufa than toV. vulgaris. The position of the genusVespa remained uncertain due to the precision limits of the RFLP technique.