变系数对流扩散方程的交替分段显-隐式方法

应用交替分段显－隐方法求解变系数对流扩散方程，此方法具有很好的并行性且无条件稳定。     

一维核废料污染问题的一个二阶格式

利用降价法研究了多孔介质中一维核废料污染问题，此模型由一非线性抛物－椭圆耦合偏微分方程组的初边值问题来描述，对此问题给出了一个关于时间和空间均具有二阶精度的差分格式，并进行了理论分析。     

如何写好统计分析

统计分析的广度、深度和力度决定于分析思路和表述思路,归结于写作思路。提高统计分析的广度、深度和力度,必须在写作思路的培养锻炼上下功夫,达到升华和提高。     

股份制企业筹资最佳资金结构的确定

最佳资金结构的确定，关系到股份制企业资金成本的高低和财务风险的大小．如何通过筹资发挥财务杠杆的作用，确定最佳资金结构是股份制企业筹资决策的关键．针对这一问题，比较详细地介绍了每股利润分析法、比较资金成本法在确定最佳资金结构中的运用．     

论会计能力模块课程体系的设计与实施

针对会计教学改革的不断深入，提出了会计能力模块课程体系的设计思路，论述了会计能力模块开发的现实性，模块设计的原则，类型及教学研究与实施方法。     

管理信息系统教学刍议

企业管理的发展变化和计算机信息技术的迅猛发展,给管理信息系统的教学提出了更高的要求,要求管理信息系统的教学体系要不断完善、教学内容要不断充实,教学方式要不断改进。从预备知识的重要性、教学内容的更新、强化案例教学、加强实践环节等方面进行探讨,探索出一条真正有效、切实可行的教学改革之路。     

互交式教学法在高校女生体育教学中的运用

根据普通高校女生心理特征,运用有关教育学理论,在高校女生体育教学中设计了互交式教学法,并与传统的教学方法进行了实验对比。     

借助Matlab遗传算法反演火山区压力源参数

首先分析了Mogi模型的经典最小二乘反演由于出现矩阵奇异或接近奇异而发散的局限性,决定采用具有全局高效搜索性和优化计算时不需要求导的遗传算法来反演火山区压力源参数。反演程序调用Matlab7.0 Release14工具箱中的选择、交叉和变异等相关程序。最后应用该程序和长白山天池火山区水准和GPS观测资料联合反演火山区压力源参数,经过比较遗传算法和阻尼最小二乘方法的反演结果,可看出2种方法结果近似,可见此方法方便快捷地实现了目标函数的最小化。     

Specific anti-tumor effect induced by attenuated Salmonella typhimurium vaccine expressing extracellular region of vascular endothelial growth factor receptor 2

The purposes of this research were to study the stable expression of exogenous gene encoding therapeutic protein in attenuated Salmonella typhimurium, observe the metabolism of oral gene vaccine carried by attenuated Salmonella typhimurium in BALB/c mouse, and investigate the feasibility of prevention and treatment of tumors by the recombinant bacteria. Recombinant plasmid pcDNA3.1 VEGFR2(n1-7) was transformed into competent attenuated Salmonella typhimurium SL3261 to develop oral DNA vaccine SL3261-pcDNA3.1 VEGFR2(n1-7). To observe whether the exogenous gene can be expressed in the recombinant bacteria, PCR was performed to amplify the CMV promoter of the eukaryotic expression vector as the proof of stable expression of exogenous protein; transmission elec- tron microscopy (TEM) was applied to observe the morphology of the recombinant bacteria to confirm that the exogenous gene has no impact on the growth of the bacteria, and then BALB/c mice were immunized with the gene vaccine. After inoculation of the gene vaccine, the recombinant bacteria SL3261 could be detected in the tissues such as small intestine, colon, liver and spleen. And then, mice in each group were challenged with tumor cells. The results of animal experiment showed that tumor growth of the mice in experimental group was inhibited and survival time of immunized mice was prolonged compared with control groups. A higher lymphocyte infiltration in tumors from animals treated with DNA vaccine was observed. Immunohistochemical analysis of tumor samples revealed an en- hanced accumulation of CD8 cytotoxic T lymphocytes, as well as an increase in CD4 cells in the tumors of animals treated with the oral gene vaccine compared to tumors from control group mice. Ultrastructure of the tumor tissue showed that tumor cells in the samples of the immunized mice were well-differentiated. Our research confirmed that the exogenous gene can be stably expressed in the attenuated Salmonella typhimurium and has no impact on the growth of the recombinant bacteria; the exogenous gene can de delivered to the host by attenuated Salmonella typhimurium to produce anti-tumor effect with no obvious cytotoxity to the host. In this study, it is established that attenuated Salmonella typhimurium could be used as a vector for oral gene vaccine, and our study provided a theoretical basis for the body distribution and the metabolism of the recombinant bacteria. This strategy may provide a simple, safe and effective way for the prevention and treatment of tumors.     

急倾斜厚煤层掩护支架采煤法机械化问题的探讨

掩护支架采煤法是开采急倾斜厚煤层的有效方法之一,但回采工艺大多使用炮采。为进一步扩大急倾斜厚煤层掩护支架采煤法的适用范固,大幅度地提高技术经济效果,必须实现掩护支架下的采煤机械化。本文在总结分析国内外有关经验、资料的基础上,对掩护支架结构型式的改进和采煤机械化问题进行了初步的探讨,提出了设想方案。