Involvement of NADPH oxidase NtrbohD in the rapid production of H2O2 induced by ABA in cultured tobacco cell line BY-2

The mechanisms for the production of hydrogen peroxide (H₂O₂) induced by abscisic acid (ABA) were investigated in suspension culture cells of tobacco BY-2 cells. The results showed that the immediate generation of H₂O₂, which was mainly derived from superoxide dismutase-catalyzed dismutation of superoxide radical, was significantly induced by ABA. Furthermore, treatment of the cultured tobacco cells with ABA resulted in a time-dependent quick increase in plasma membrane (PM) NADPH oxidase activity, which coincided on time and magnitude with the elevation in ABA-induced accumulation of H₂O₂. Moreover, these enhanced effects were pronouncedly inhibited by two NADPH oxidase inhibitor, diphenylene iodonium and imidazole, suggesting that PM NADPH oxidase is involved in the rapid accumulation of ₂O₂ in cultured tobacco cells. In addition, analysis of the expression level of NtrbohD, a PM NADPH oxidase gene in tobacco, by RT-PCR and protein gel blot revealed that the gene at both mRNA and protein levels was upregulated by ABA, indicating that NtrbohD participates in the ABA-stimulated rapid production of H₂O₂ in tobacco culture cells. Taken together, these findings suggest that ABA induces the rapid accumulation of reactive oxygen species via NADPH oxidase in suspension culture cells of tobacco, and that NADPH oxidase and H₂O₂ appear to be important components in ABA signal transduction pathway in plants.     

烟草行业CIMS集成化支撑保障体系

分析了烟草行业信息化建设当前存在的问题,提出采用CIMS技术实现烟草行业现代集约化管理.基于烟草行业CIMS的应用系统集成模式构建行业CIMS集成支撑保障体系,它包括行业集成支撑体系和行业集成保障体系.行业集成支撑体系负责行业CIMS应用系统的数据集成、应用服务集成和业务过程集成,行业集成保障体系负责为行业CIMS应用系统集成提供标准化体系与安全体系等保障机制,保证系统的安全高效率运行.实际应用扩充了CIMS内涵,将其集成范围从传统的制造企业扩展到工商贸,直至烟草整个行业,行业集成数据中心提供标准化管理体系、信息安全管理体系与行业集成技术支撑体系三大类功能,是行业集成的支撑保障体系,为烟草行业各级部门的集约化管理服务.     

凉山彝族兰花烟

本文从生物学特性、传统习惯、历史史实等方面，对凉山彝族吸食的兰花烟进行考证和研究，并对立山彝族传统文化中不健康的烟文化内容，结合现代思想进行了批判。     

基于JSP技术的网上烟草经营系统设计

针对网络购物这种以高效、低成本的优势成为新兴的经营模式和理念的电子商务形式,提出了基于JSP(Java Server Pages)技术在网络上架构一个烟草经营管理系统,是一个动态、交互式、具有商品预售、系统管理、用户留言等功能的电子商务网站.     

胰蛋白酶抑制剂抗虫基因转化烟草的研究

实验用携带质粒pAM194/TI的根癌农杆菌LBA4404转化烟草,通过筛选,共获得35株卡那霉素抗性苗.GUS组织染色、PCR检测及抗虫试验表明胰蛋白酶抑制剂抗虫基因已经成功地整合进再生植株染色体基因组中,有些已正确表达.     

基于滑模与状态反馈模糊加权控制的气动伺服焊枪柔性接触研究

在焊枪电极接近运动过程中,期望接近速度大且在接触面附近快速降速以减小接触时的冲击力,实现一种柔性接触,对于提高焊接的效率和质量具有很重要的作用.滑模控制虽然能有效降低焊枪电极接触力,但所用时间较长.本文将基于模糊逻辑的滑模与状态反馈加权控制运用到气动伺服焊枪系统中,在建立系统模型的基础上设计滑模切换函数和滑模控制律,通过极点配置的方法设计状态反馈控制器,之后基于模糊控制设计滑模与状态反馈模糊加权控制器,最后通过实验验证提出的控制策略.实验结果表明设计的控制器控制效果优于最大量控制和滑模控制,可有效实现柔性接触.     

烟草物流输送线上分箱装置的设计与应用

成品物流自动输送系统上的烟箱在进入提升机等节点设备工位易出现卡烟箱现象。设计了一种输送线烟箱分隔装置,对机械装置中的驱动方式、横档材料以及横档结构进行了优化设计,采用检测定位、PLC技术实现电气控制设计,最终使进入提升机的件烟箱保持一定合理间距。经实际应用,改进前平均每月卡烟箱5~6次,改进后完全杜绝了卡烟箱现象,提高了设备运行效率。     

轮胎压路机压实热拌沥青混合料的机理和作用

应用力学原理,研究了轮胎压路机在碾压热拌沥青混合料时充气轮胎与沥青混合料的相互作用,进一步分析了充气轮胎在压实过程中的揉压机理和作用,并对轮胎压路机与光轮压路机及振动压路机在压实沥青混合料摊铺层时的性能进行了对比研究。结果表明,轮胎压路机压实沥青混合料摊铺层能得到更高的压实质量,应选用轮胎压路机作为沥青混合料复压的主要机械。     

入世后国内烟草业与国外烟草业博弈分析

针对当前国内烟草业的现状，根据入世的特点，提出了一种完全信息动态博弈模型，该模型对于我国烟草业在入世后如何面对具有强大竞争实力的国际大公司有借鉴作用。     

Increment of antioxidase activity of transgenic tobacco with betaine aldehyde dehydrogenase

Superoxide dismutase (SOD) activity in the leaves of transgenic tobacco plants with betaine aldehyde dehydrogenase (BADH) gene was about 36% higher than that in the control plants (parent plants), activities of peroxidase (POD) and catalase (Cat) increased by about 62% and 88% respectively. Activities of ascorbate peroxidase (AsSPOD), dehydroascorbate redutase (DAsAR) and glutathione reductase (GR) in ascorbate-glutothion pathway located at chloroplasts increased by 67.7%, 47.9% and 38.8% respectively. These results indicated that the H₂O₂ produced by SOD catalyzing superoxide anion radicals (O^-₂) could be fully decomposed, and could not derive to form the strongest toxicant radicals ·OH. This is the first report to elucidate quantitatively that the activities of two kinds of antioxidative enzymes decomposed radicals and active oxygen were matched. Photoinhibition tolerant capacity of the transgenic tobacco plants was 35% higher than that in the parent plants. Increment of photoinhibition tolerant capacity in the transgenic tobacco plants might be due to increment of antioxidative enzymes activities, in turn being able to more effectively scavenge active oxygen and radicals, protect organization and function of chloroplasts. These results showed that the increment of antioxidative enzymes activities in the transgenic tobacco might be one of the reasons for the increment of resistance in the transgenic tobacco.