内蒙古地区2型糖尿病患者危险因素与脂联素基因多态性交互作用研究

目的：探讨内蒙古地区2型糖尿病患者危险因素与脂联素基因rs266729多态性交互作用。 方法：病例组来源于内蒙古医科大学附院以及内蒙古中蒙医院糖尿病患者共111例,对照组来源于内蒙古医科大学附属医院体检中心共106例。运用PCR-RFLP技术检测脂联素（ADIPOQ） 基因rs266729位点多态性。采用二元logistic回归方法分析脂联素基因rs266729多态性与T2DM的相关性,并且与环境因素进行交互作用分析。结果： ADIPOQ 基因rs266729多态性等位基因频率分布符合 Hardy-Weinberg 定律; T2DM基因型频率分布依次为 CC: 85.61% CG : 5.42%GG : 9.02%。校正 BMI之后,CG基因型人群是 CC基因型人群患T2DM的0.260倍,且有显著性意义( p=0.009),而 GG基因型人群是 CC基因型人群患 T2DM的1.146倍, 无显著性意义( P=0.790)。 交互作用分析结果表明： rs266729位点与空腹血糖存在正交互作用;与收缩压、空腹 C肽、空腹胰岛素、生活环境存在负交互作用。结论：脂联素基因 rs266729多态性可以增加个体2型糖尿病罹患风险,且rs266729基因多态性与与空腹血糖、吸烟、酗酒、日常运动、日常情绪、生活紧张度、腰臀比、超重、糖尿病家族史这几项存在正交互作用,与收缩压、空腹 C肽、空腹胰岛素、生活环境存在负交互作用。     

Partial sequence of the gene for a serine protease from a halophilic archaeumhaloferax mediterranei R4, and nucleotide sequences of 16S rRNA encoding genes from several halophilic archaea

A part of the gene coding for a halophilic serine protease from a halophilic archaeumHaloferax mediterranei R4 was amplified by PCR and its 672 nucleotide sequence was determined. Tentative translation to the amino acid sequence suggested that the enzyme was quite similar to halolysin produced by another halophilic archaeum strain 172P1. Nucleotide sequences of 16S rRNA encoding genes from 9 halophilic archaea were determined. Alignment of 19 sequences known so far showed that there are more than 20 positions carrying bases or deletions specific for each halobacterial genus:Halobacterium, Haloarcula, Haloferax, andHalococcus.     

牦牛核苷酸序列资源的调查

目的了解牦牛核苷酸序列的资源状况,为今后的相关选题和研究提供重要参考.方法根据GenBank的序列记录,从登录号、记录名称、序列长度、DNA/mRNA、接受日期、完整/不完整编码区序列、递交机构7个项目进行统计,分析数据量和增长率、数据的递交机构、覆盖的基因和区域、包含完整CDS的记录4个方面的情况.结果从1995年至2005年,共有19个机构递交了相关序列,总碱基数达160321 bp,这些序列覆盖了65种不同基因或区域.     

The Statistical Correlation of Nucleotides in DNA Sequence and Its Biological Meaning

评述了ＤＮＡ序列碱基关联的统计研究，着重分析了编码区关联的短程为主性及有关信息参数的进化相关性，讨论了碱基关联的生物学应用．     

Integration of rolling circle amplification and cationic conjugated polymer for the homogeneous detection of single nucleotide polymorphisms

A novel, homogeneous and sensitive assay for the detection of single nucleotide polymorphisms (SNPs) by integration of rolling circle amplification (RCA) and cationic conjugated polymer (CCP) has been developed and tested. Mutant DNA serves as the template for specifically circularizing a padlock probe (PLP) with a sequence that is complementary to the mutant DNA. Afterwards, the mutant DNA directly acts as the primer to initiate the RCA reaction in the presence of phi29 DNA polymerase that generates a long, tandem single-strand DNA product. During the RCA reaction, fluorescein-labeled dUTPs are incorporated into the RCA products. When the CCP is introduced, efficient FRET from CCP to fluorescein occurs as a result of the strong electrostatic interactions between the CCP and the DNA produced by RCA. The wild-type DNA contains a single base mismatch with PLP with the result that the PLP is not circularized, RCA is not triggered and inefficient FRET results. By measuring the change of the emission intensities of CCP and fluorescein, it was possible to detect the SNP in a homogeneous manner. The method is sensitive and specific enough to detect 0.1 pmol/L mutant DNA and to determine a mutant allele frequency as low as 2.0%.     

食用菌鲜味强度评价及鲜味氨基酸和核苷酸提取工艺优化

通过高效液相色谱对9种食用菌的鲜味氨基酸、核苷酸含量进行检测并计算等鲜浓度(EUC)值,再结合感官分析和电子舌技术评价9种食用菌的鲜味强度。结果表明:鹿茸菇在9种食用菌中的感官评分和电子舌的鲜味评分值最高,EUC值为160.56 g/100 g,因此选用鹿茸菇为原料进行酶解提取工艺研究,以EUC值为考察指标,选用纤维素酶和风味蛋白酶复合,通过单因素实验和响应面法对鹿茸菇中鲜味氨基酸和核苷酸提取工艺进行优化,得到食用菌的优化酶解条件为:料液比(g/mL)1∶44.78、纤维素酶添加量1 607.52 U/g、风味蛋白酶添加量3 044 U/g、酶解时间6.14 h、pH值6.50。以优化条件进行3次平行实验,得到EUC实际值为406.74 g/100 g,与预测值相对误差为6.12%。     

广东汉族人群TLR9基因单核苷酸多态性及其单倍型(英文)

采用PCR扩增和直接测序法对191例健康且无血缘关系的广东汉族人群筛查了TLR9全基因序列,包括调控区、5′非翻译区、第1,2外显子、内含子及3′非翻译区上所有的单核苷酸多态性(SNP)位点.共检出五个SNP位点,分别为调控区的-1 486 T/C和-1 421 C/T、内含子区的+1174 A/G、第2外显子的+1 387 T/C和+2848 G/A,其中-1421 C/T和+1 387 T/C为首次发现的新位点.连锁不平衡分析表明-486 T/C,1174 A/G以及2848 G/A之间存在紧密连锁,并且涵盖了整个基因区域,形成了一个单倍域.在此基础上运用Hhase软件构建了TLR9基因的单倍型,共得到七种单倍型并模拟了它们可能的分布频率.进一步的中性检验表明TLR9基因在广东汉族人群中符合中性进化模式.     

全基因组关联分析显示基因ANXA8和C10orf11为影响肌少症的候选基因

为了寻找与瘦体重(lean body mass,LBM)相关的单核苷酸多态性(single nucleotide polymorphism, SNP)位点及易感基因,在1 000个不相关的白人中采用Affymetix 500K芯片扫描了500 000个SNPs,并进行全基因组关联分析(genome-wide association study,GWAS),显著结果在1 625个中国人样本和2 283个欧洲白人样本中进行验证,并将验证结果与研究结果进行荟萃分析。研究发现SNPsrs7905603,rs9416083,rs4409772,rs2894310与LBM关联,其中rs7905603位于基因ANXA8,其他3个SNPs位于基因C10orf11。荟萃分析得到的合并p值分别为2.08×10-5,7.44×10~(-6),6.73×10~(-6),6.76×10~(-6)。ANXA8和C10orf11基因是影响LBM变异的候选基因,这对肌少症的认识提供了新的理论依据。     

Cloning,Sequencing and Phylogenetic Study of rbcL Gene from Cyanobacteria Arthrospira and Spirulina

Large subunit gene of rubisco (rbcL) of cyanobacteria Arthrospiraplatensis FACHB341, A. platensis FACHB439, A. maxima OUQDSM and Spirulina sp. FACHB440 is cloned, sequenced and characterized. Results show that GC content of the gene in strain Spirulina sp. FACHB440 is higher than that in the others. The alignments based on deduced amino acid sequences indicate that Spirulina sp. FACHB440 is different from that in other three samples of Arthrospira, though they have the same conserved functional sites (95, 98, 121, 124, 221, 257). The nucleotide sequence similarity among the three strains of the genus of Arthrospira (96.5 - 99.6% ) is higher than that between Arthrospira and Spindina (78.1 - 78.5 % ). By comparison of the corresponding sequence of other cyanobacteria, a phylogenetic tree with two clusters is constructed. A. platensis FACHB341, A. maxima OUQDSM and A.platensis FACHB439 form the monophyletic linage, which is fully supported by bootstrap values (1000), while Spirulina sp. FACHB440 and Anabaena sp. PCC7120 cluster in another linage with the bootstrapvalue of 909.     

甲状腺素对金黄地鼠肝LDL受体影响的研究

报道了甲状腺素对金黄地鼠血脂和肝ＬＤＬ受体的影响，探讨了甲状腺素降低血清脂质的机制．雄性金黄地鼠３０只随机分为对照组和甲状腺素组，前者喂基础饲料加０．１２％的胆固醇，后者再加喂０．１０％的甲状腺素．３５天后，测定血脂和肝脏ＬＤＬ受体．结果表明，与对照组相比，甲状腺素组血清ＴＧ、ＴＣ、ＬＤＬ－Ｃ和ＶＬＤＬ－Ｃ均明显下降，ＨＤＬ－Ｃ虽有升高，但尚无显著性差异．体外结合实验发现，甲状腺素组金黄地鼠肝匀浆与１２５Ｉ－ＬＤＬ的特异性结合位点（３８．０３±１．０３ｎｇ１２５Ｉ－ＬＤＬ／ｍｇ肝匀浆蛋白）显著高于对照组（２６．２０±１．９２ｎｇ１２５Ｉ－ＬＤＬ／ｍｇ肝匀浆蛋白），而１２５Ｉ－ＬＤＬ与两组动物肝匀浆蛋白结合的亲和力无明显差异，提示甲状腺素降低血脂与肝ＬＤＬ受体增加密切相关．