A novel multi-functional cell-based microphysiometer

This paper presents a novel multi-functional microphysiometer for simultaneous measurements of several extracellular ion concentrations and action potential measurement in living cells based on MLAPS (multi-light addressable potentiometric sensor). In the microphysiometer, sorts of sensitive membranes are illuminated in parallel with n light sources at working frequencies, and the response amplitudes of each frequency component can be measured on-line by parallel processing algorithm. In the experiments, the relations of the extracellular environmental H⁺, Na⁺, K⁺, Ca²⁺ under the effects of western medicines (dilantin, phenobarbital sodium) and Chinese drugs (scutellaria, medlar, hemlock parsley) were analyzed, and the effects of several drugs were evaluated. Moreover, the action potential signals of different cell types (cardiac myocytes and neurons) could be measured and analyzed by LAPS. By detecting these parameters, the system can monitor the real-time process of the cell metabolism and action potential, observe the functional responses of different kinds of membrane-bound receptors, and evaluate the activities of drugs.     

Gene flow from genetically modified herbicide-resistant rapeseed to cruciferous weeds

The sexual compatibility between genetically modified (GM) glyphosate-resistant rapeseed variety Q3 (Brassica napus L.) and 5 cruciferous weeds is studied through the observation of fluorescence microscopy and cross-fertility after manual pollination. The results indicated that Q3 (as male) was highly incompatible with Thlaspi arvense L., Capsella bursa?pastoris (L.) Medic, Cardamine hirsuta L. and Rorippa palustris (L.) Besser (as female). Fluorescence microscopic observation showed that growing of pollen tubes terminated on the stigma surface or at the upper 1/3 part of the style. However, B. juncea×Q3 was compatible, and the compatibility index was 1.65. Under the neighboring growth and natural pollination conditions, the rates of gene flow from Q3 to T. arvense, C. bursa-pastoris, C. hirsute and R. palustris were all 0, while it was 0.86% for B. juncea. These results indicate that there is difference in the rate of gene flow between GM rapeseed and cruciferous wild weeds, and frequency of gene flow is highly correlated with sexual compatibility.     

Research on an active and continuous monitoring system for human respiratory system

Continuous and dynamic measurements of human respiratory parameters are very important for vital diseases of respiratory system during mechanical ventilation. This paper analyzed the structure and mechanical properties of the human respiratory system, and designed an active intervening monitoring micro system for it. The mobile mechanism of the micro system is soft and earthworm-like movement actuated by pneumatic rubber actuator, the measurement and therapy unit of the system is an extensible mechanism with sensors in the front. The micro monitoring system can move in respiratory tract and measure the respiratory parameters in bronchium continuously. Experiments had been done in swine＇s respiratory tract, the results proved that the micro robot system could measure the respiratory parameters in real-time successfully and its movement was smith in swine＇s respiratory tract.     

柴油蜡晶分散剂的制备和降凝效果研究

以马来酸酐和呋喃为原料，经Diels-Alder反应、酯化／酰胺化后合成了柴油蜡晶分散剂3，6-氧桥-1，2，3，6-四氢苯-1，2-二甲酸高级酯／酰胺。酸值测定表明，该合成方法的产率超过90％。对大庆0^#柴油的冷滤点测试表明，该醋晶分散剂与低相对分子质量的乙烯-醋酸乙烯酯共聚物复配效果较差，而与改性的聚丙烯酸高级醇酯二元复配后，可以降低冷滤点7℃，表现出了良好的协同效应。     

直接流动注射化学发光法测定头孢噻肟钠

以多聚磷酸作为反应介质,采用高锰酸钾氧化头孢噻肟钠能产生强的化学发光,结合流动注射技术,建立一种简单、灵敏、稳定的流动注射化学发光测定头孢噻肟钠的新方法.探讨反应介质、多聚磷酸浓度、氧化剂高锰酸钾浓度及载液流量对化学发光的影响.研究结果表明,在6.0×10-2mol/L多聚磷酸介质中,以7.0×10-4mol/L高锰酸钾做氧化剂,载液流量为6.34 mL/min时,该法具有最佳灵敏度.在最优实验条件下,该法的线性范围为0~450μg/mL,其线性回归方程为ICL=0.983C 0.875(r=0.999 9,n=11),检出限为1.9μg/mL.对50.0μg/mL头孢噻肟钠进行11次平行测定,方法的相对标准偏差为0.5%.用本法对针剂中头孢噻肟钠的含量进行测定,回收率在98.4%~101.0%之间.初步探讨该化学发光反应的发光机理.     

螺型位错与含共焦裂纹弹性椭圆夹杂的干涉效应

研究了基体与夹杂中任意位置螺型位错与含共焦裂纹弹性椭圆夹杂的干涉问题,运用复变函数的分区全纯理论、柯西型积分、应力函数奇性主部分析方法与Rieman边值理论,将问题归结为一个初等复势函数方程的求解.获得了基体与夹杂区域复势函数的级数形式精确解,导出了裂纹尖端应力强度因子解析表达式和作用于位错的像力公式.计算结果表明:夹杂中的裂纹对于位错与夹杂的干涉具有强烈的扰动效应,它增强软夹杂对位错的吸引,减弱硬夹杂对位错的排斥,甚至将排斥转变为吸引.裂纹尖端附近的应力强度因子等值线表明,螺型位错位于裂纹尖端附近特定区域时对于裂纹扩展具有屏蔽效应.     

求解电力系统潮流方程全部解的LINGO方法

针对现有电力系统潮流方程全部实数解求解方法的不足,提出了一种用LINGO10.0软件求出全部解的方法。该方法是用LINGO10.0求出一个解后,将该解作为一个约束,使新解与该解不同,直至求出全部解。该方法简单、实用,为潮流方程全部解求解提供了全新的方法。     

浅谈如何进行有效的绩效管理

阐述了绩效管理的含义,针对企业在绩效管理的实际操作中存在的问题,探讨了如何有效地进行绩效管理。     

首都国际机场3号航站楼屋面雪荷载分布研究

采用两相流理论模拟风荷载雪漂作用,认为空气相和雪相的关系为单向耦合,雪在风(空气相)的作用下发生漂移.对通用计算流体动力学软件FLUENT进行了二次开发,计算了在风作用下积雪发生迁移后屋盖表面的雪压分布,并对雪荷载改变量以及雪压分布的规律进行了分析,为结构设计提供了依据.     

液压挖掘机工作装置的自适应控制

首先介绍了试验挖掘机机器人的电液比例系统及其控制系统的硬件结构,结合所采用的负载独立分配(LUDV)系统的特点给出了电液比例系统的数学模型,在此基础上,根据试验结果对电液比例模型进一步进行简化,得到了设计控制器所需的参考模型.然后以局部参数最优法为基础设计了自适应控制器,给出了自适应控制律.最后对铲斗轨迹跟踪控制进行了试验研究,通过试验结果对所设计的控制器与常规比例-积分-微分(PID)控制器跟踪设定直线的效果的比较分析,可知使用PID控制器所得到的跟踪误差大于110 mm,而使用所设计的控制器其跟踪误差可以控制在100 mm之内,精度有了一定的提高,并且铲斗运动速度从107 mm.s-1提高到135 mm.s-1,非直线度从4.8%降为3.8%,从而验证了所设计控制器的正确性和有效性.