基于SPSS因子分析的养猪废水中藻生长适应性综合评价

基于SPSS软件的因子分析法,对小球藻、栅藻、微囊藻3类藻在养猪废水8种不同处理阶段的水样中进行藻生长适应性综合评价研究,旨在为分析评价藻在养猪废水中的生长适应性、养猪废水作为藻类培养基的可能性.研究结果表明:8种水样中原水的营养成分较适合藻生长,3种藻类中小球藻生长的综合适应能力最强,小球藻能在养猪废水中生长成为优势藻种.     

家猪mtDNA多态性及其应用

概述了家猪线粒体DNA(mtDNA)的一些特点 ,着重讨论了家猪mtDNA多态性的最新研究成果 ,详细论述了mtDNA多态性在家猪的起源、进化、分类以及群体遗传结构分析等方面的应用 ,并预测了家猪mtDNA研究的发展趋势     

Phenotypic and Functional Analysis of Porcine T Lymphocytes

IntroductionPorcine is a useful model for the heterogeneity ofmammalian immune systems and has also receivedattention as a possible source of organs for humantransplants[1] .T lymphocytes are an importantcomponent of immune cells which directly inducecellular immune response and play a key role inimmune regulation.The cognition,activation,andfunction of T lymphocytes depend on interactionsbetween T cells and antigen presenting cells,aswell as B lymphocytes and other cells.The basesfor the in…     

猪粪干式厌氧消化系统氨氮变化规律及影响

在中温(36℃)条件下,进行了猪粪干式厌氧消化中试试验,以探索系统运行过程中氨氮的变化规律。研究表明,厌氧微生物没有足够的驯化时间,对氨氮的耐受力较低。为促进厌氧微生物对高浓度氨氮的耐受力,进行消化液回流以逐渐提高氨氮浓度。但是持续快速升高的氨氮浓度会造成氨氮抑制,为防止这种现象的发生,对出料进行固液分离后只进行沼渣回流,氨氮浓度稳定在5 000 mg·L~(-1)。为了进一步考察氨氮的变化规律再次变为回流消化液,系统氨氮浓度逐渐升高达到6 000 mg·L~(-1),沼气产量由38 m~3·d~(-1)降为28 m~3·d~(-1)。说明沼渣回流比消化液回流对系统氨氮浓度能起到更好地控制作用。另外,酸碱比(VFA/TA)是否能作为系统稳定性指标,也会受到氨氮浓度的影响。     

国产氯前列烯醇和催产素诱导母猪分娩效果初探

临床试验表明,对妊娠111天的长×约母猪单独肌注国产氯前列烯醇0.1mg后平均34.23±6.80h(n=30)开始分娩,分娩持续时间为3.29±1.20h,与对照组84.24±13.48h(n=30)和4.23±2.48h相比前者差异极显著(P<0.01),后者差异较显著(P<0.05)。应用氯前列烯醇24h后配合肌注催产素10iu,其开始分娩的时间从初用药计算为29.39±5.16h(n=30),平均产仔时间为2.94±1.67h,两组数据与对照组相比均差异极显著(P<0.01),与单独用药组相比开始分娩的时间提前约4.8h,差异极显著(P<0.01),分娩持续时间缩短1.29h,差异显著(P<0.05)。各组间平均窝产仔数和平均窝产死仔数的差异均不显著。两种激素配合使用比单独用药分娩时间更趋于集中,产后下奶良好,可作为一种控繁手段准确诱导母猪于白日分娩,达到提高管理分娩母猪质量,减少仔猪死亡头数的目的。     

Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain

Swine vesicular disease (SVD) is a highly conta- gious viral disease of pigs. Symptoms are clinically indistinguishable from those caused by other vesiculardisease viruses, such as foot and mouth disease (FMD virus, vesicular stomatitis (VS) virus and ves…     

猪作为异种器官移植供体的研究进展

异种器官移植(Xenotransplantation,XT)是未来生物学科中的重要研究领域之一.猪作为为人类提供移植器官的供体源具有得天独厚的条件.文章就器官移植(OrganTransplantation,OT)的历史、原因、移植后的免疫排斥反应和猪内源性逆转录病毒(porcine endogenous retrovirous,PERV)感染问题及可能的解决途径进行了综述.     

ELISA 抗原检测与荧光 RT-PCR快速诊断猪瘟的比较研究

为找出临床上猪瘟快速、有效的诊断方法,对荣昌县2个猪场出现的40头临床症状疑似猪瘟的病猪进行病理剖检,结果证实有8头病猪出现猪瘟典型症状.采集40头病猪的血样,进行ELISA猪瘟抗原检测和荧光RTPCR猪瘟核酸检测,结果显示ELISA抗原检测检出7份血样为猪瘟抗原阳性,阳性检出率为87.5%;荧光RTPCR核酸检测出4份血样为阳性,阳性检出率为50%.该结果证实,ELISA猪瘟抗原检测的阳性检出率比荧光RT-PCR核酸检测更高,建议临床上采用ELISA抗原检测的方法进行猪瘟的快速诊断.     

不同杂交组合猪繁殖性状的杂种优势分析

根据青海八眉猪（本）、长白（长）、大白（大）及其长×本、大×本和长×长×本杂交组合的繁殖性状记录,研究了3种杂交组合繁殖性状的杂种优势。结果表明：长×本、长×长×本杂交组合繁殖性状各指标均表现有杂种优势,尤其以长×本杂交组合的产仔数、产活仔数优势明显;以长×长×本杂交组合的断奶窝重、断奶重、20日龄窝重、20日龄重、初生窝重等性状优势明显;大×本杂交组合繁殖性状的杂种优势最低,部分性状不表现杂种优势。     

Construction of cDNA library, nucleotide sequence and analysis of entire genome of classical swine fever virus strain Shimen

According to the previously published CSFV sequences, 18 pairs of primers have been designed and synthesized, which cover the entire genome of CSFV strain Shimen. Each cDNA fragment has been amplified by RT-PCR from the anticoagulant blood of strain Shimen infected pig. The PCR products have been cloned respectively and sequenced. Results show that the cDNA library of strain Shimen and its nucleotide sequence have been obtained. The genomic RNA of strain Shimen is 12 298 nucleotides in length, containing a 5′ and a 3′ noncoding region 373 and 231 nt long respectively. The center of genome is a single large open reading frame of 11 697 nt which encodes a polyprotein of 3 898 amino acids. The entire sequence of strain Shimen has also been compared with that of other CSFV strains.