仔猪黄痢的诊断与防治

对2005年4月内蒙古通辽市某猪场初生仔猪发生不明原因的泛性腹泻进行了研究.根据流行情况、临床症状、病理变化和实验室检验,确诊此病是由大肠杆菌引起的仔猪黄痢,提出了正确的治疗措施,治疗结果较好.     

黄河无定河流域分布式时变增益水文模型的应用研究

黄河无定河流域是典型的黄土高原沟壑地区，水文时空变化大，观测站点有限，水文过程的非线性问题和人类活动影响问题比较突出．本文针对黄河无定河流域内不同尺度水文循环模拟问题，对作者开发的分布式时变增益水文模型进行了改进，拓展到了月、日、时三个时间尺度，应用于无定河内的大理河、小理河及岔巴沟三级不同空间尺度的流域．研究表明：分布式时变增益水文模型是水文系统非线性理论与分布式流域水文模拟结合的一种新途径，水文系统模拟的精度比较满意．该模型为分析土壤湿度的时空分布与变化以及人类活动的影响，提供了一种量化分析的有效工具．     

Clone of Chinese Jinan red-cross yellow cattle and evaluation of reproductive characteristics of cloned calf

Somatic cell clone technology is a viable approach to preserving endangered livestock and wildlife genetic resources. In the present research, somatic cell nuclear transfer （SCNT） was performed using granulose cells from the critical endangered Chinese red-cross yellow cattle as donor cells. A total of 211 oocytes were manipulated and 166 （79%） of them were successfully enucleated. 112 （67.4%） SCNT embryos were reconstructed, 94 （83%） of them cleaved, and 48 （43 %） of them developed to blastocyst stage. SCNT blastocysts were transferred to 6 Holstein recipients, and 2 （33%） of them were found to be pregnant. One of them maintained to term and delivered a calf, whereas another aborted. Effect of different fusion buffer （mannitol vs. Zimmerman fusion buffer） and different activation methods （calcium ionophore＋6-DMAP vs. cycloheximide＋CB） on fusion rate and development of SCNT embryos were investigated. The results indicated that： （i） on condition of two DC pulses of 2.5 kV/cm for 10 μs each, fusion rates were higher in mannitol solution than in Zimmerman fusion buffer （71% vs. 61%, respectively, p 〈 0.05）, but the blastocysts rates did not differ between two treatments （36 % vs. 39 %, p〉0.05 ）; （ii） There was no significant difference in development rates to the blastocyst stage for SCNT embryos activated by calcium ionophore＋6-DMAP or by cycloheximide＋CB （42% vs. 46%, respectively, p〉0.05）. Microsatellite DNA analysis examining 28 loci confirmed that the cloned calf was genetically identical to the donor Jinan red-cross yellow cattle and different from the recipient females. Growth and reproductive performance of cloned cow were evaluated, and there were no difference i cross-red n it between cloned and normal control Jinan yellow cattle. Furthermore, the cloned yellow cow has delivered a healthy yellow calf.     

柑桔果皮的深加工与综合利用

桔皮精油、色素、果胶、橙皮甙是目前桔皮的几种主要深加工产品,在化学工业和食品工业中有很高的应用价值.分别从它们的物理化学性质、功能特性、提取方法及影响产率的主要因素等方面进行综述.     

非掺杂GaN薄膜的黄光发射机理研究

提出了一种新的光谱———吸收归一化光致发光激发谱 ,并用这种光谱研究了非掺杂GaN薄膜的黄光发射。实验上首次直接测出了黄光发射的初始态的共振吸收峰位 ,从而解决了长期争论的有关黄光发射的发光机理问题     

南黄大麦根细胞的超微结构简报

对南黄大麦突变系及其野生型早熟３号的根细胞进行电镜观察，结果表明，二者根细胞的超微结构无明显差异。均有较大的细胞核和核仁，核仁内有核仁液泡，这是细胞代谢活动旺盛的表现。     

藉催化氧化酸性间胺黄反应动力学光度法测定痕量钒

在硫酸介质中，以酒石酸为活化剂，钒对溴酸钾氧化酸性间胺黄的反应具有极强的催化作用，对催化作用的适宜条件进行了研究，并以此反应为指示反应，建立了测定痕量钒的动力学光度法新体系，测定线性范围为0～6ng／mL，检出限为0．15ng／mL．已应用于人发及粮食中痕量钒的测定．     

Molecular characterization of<Emphasis Type="Italic">Tomato yellow leaf curl China</Emphasis> virus and its satellite DNA isolated from tobacco

Virus isolates, Y8, Y36 and Y38, were obtained from tobacco plants showing leaf curl symptoms in Honghe,Yunnan Province. In reactions with 14 monoclonal antibodies raised against Begomoviras particles, Y8, Y36 and Y38 had similar antigenic reaction in TAS-ELISA as Tomato yellow leaf carl China virus (TYLCCNV). The complete DNA-A nueleotide sequences of Y8, Y36 and Y38 were determined and they contain 2727, 2730 and 2730 nueleotides, respectively. Each of the DNA-A sequences has a typical Begomovirus genome organization encoding 60RFs with 20RFs[AVI(CP) and AV2] in virion-sense DNA and 40RFs (AC1 to AC4) in complementary-sense DNA. Comparisons with total DNA-A, intergenie region and deduced amino acid sequences of individual ORFs show that Y8, Y36 and Y38 are isolates of TYLCCNV. Satellite DNA molecules (DNAβ) were found to be associated with Y8, Y36 and Y38, which consist of 1338, 1339 and 1338 nucleotides, respectively. Comparisons show that these DNAβ molecules share 98%--99% sequence identities on nucleotide level and have a common ORF (designated C1) encoding 126 amino acids on the complementary strand.     

The sensitive period for yellow phenocopy induction inDrosophila melanogaster

Summary Yellow phenocopies ofDrosophila melanogaster were produced by raising larvae on -DMT contaminated media. Using a survivorship test, the sensitive period for phenocopy induction was found to occur during the third larval instar of development, with increased survivorship at 1% -DMT compared with lower concentrations. It was also found that treatment with -DMT significantly slowed development. These findings are related to the relevant morphological and behavioral developmental pathways and to phenocopy induction.Publication No. 35 from the Evolutionary Genetics Laboratory, University of Auckland.Acknowledgments. We thank the New Zealand Entomology Society for a grant from their Anniversary Fund to R. D. Newcomb and the University Grants Committee, Research Grant No. 394.597 to D.M. Lambert.     

过氧化物酶与不同甜瓜品种对小西葫芦黄化花叶病毒抗性的关系

研究了对小西葫芦黄化花叶病毒新疆株（Zucchiniyellowmosaicvirus-Xinjiangstrain,ZYMV）抗性不同的甜瓜品种受病毒感染后过氧化物酶活力及其同工酶谱的变化．与健康对照相比,受感染植株的酶活力均有所增高,但不同抗性品种受感染植株酶活力增高的幅度、酶活力高峰出现的时间和同工酶谱带的增多,均表现与抗性相关的规律性差异．抗性品种“新玉”的酶活力增高幅度是感性品种“网纹香”的2倍,酶峰早出现12d;2条只在感染植株中出现的同工酶带C和E在“新玉”中较“网纹香”中早出现15d,并多维持12d．抗性居中的品种“红心脆”和“向里黄”的以上指标则居“新玉”和“网纹香”之间．2条健康对照和感性品种都不存在的酶带H和I,则仅在抗性较强的品种中出现．