威宁鸡与贵州黄鸡血清酯酶-1的多态性比较研究

本文采用聚丙烯酰胺凝胶电泳对贵州优良地方鸡种威宁鸡48份血清蛋白的酯酶(ES-1)位点电泳图谱的检测,并与贵州黄鸡相应位点的比较研究发现:威宁鸡的酯酶-1呈现明显的多态现象。两个品种的ES-1A频率都很低。     

黄磷生产自动化控制系统设计

针对黄磷生产装置及操作工艺条件,给出了基于PLC的自动化控制系统的设计,包含控制模块选型、通迅方式、系统网络结构、上位机组态及工业电视监视系统,形成了完整的自动化控制中心.实现了黄磷生产的自动化控制,降低了生产工人的劳动强度,提高了生产装置的效率.     

Function of A-Rich region in DNAβ associated with Tomato yellow leaf curl China virus

A novel type of circular single-stranded satellite DNA, known as DNAβ, was recently characterized and demonstrated to be associated with monopartite begomoviruses. DNAβ was essential for induction of characteristic symptoms in plants. DNAβ has three structural features: an 115 bp highly conserved region, tiC/gene and A-Rich region. The in-frame ATG mutation of βC1 gene of Tomato yellow leaf curl China virus isolate Y10 (TYLCCNV-TY10) DNAβ demonstrated that βC1 gene is required for leaf curl symptom. Here, the function of A-Rich region in TYLCCNV-Y10 DNAβ was identified. When A-Rich region was deleted, the A-Rich deleted mutant was still capable of replication and systemic infection in plant, indicating that A-Rich region is not required for trans-replication of DNAβ. The immunotrapping-PCR demonstrated that A-Rich deleted mutant could be encapsidated in the coat protein encoded by TYLCCNV-Y10 DNA-A, suggesting that A-Rich region is not related with DNAβ encapsidation. However, the A-Rich region deleted mutant caused milder symptom.     

Identification of a bi-directional promoter from Tomato yellow leaf curl China virus

A bi-directional promoter of Tomato yellow leaf curl China virus (TYLCCNV) was obtained with the total DNA from TYLCCNV isolate Y10 infected tobacco leaves as a template. Plant expression vectors were constructed by fusing the amplified DNA fragment with the gus gene and nopaline terminator in different orientations. The vectors containing promoter fragments were transferred into leaf cells and plant stems of Nicotiana benthamiana by Agrobacterium-mediated method. Transient expression results showed that both the complementary and virion-sense promoters could drive the gus gene to express, and the GUS activity of the complementary-sense promoter was stronger than that of the virion-sense. Co-expression of the vector containing βC1 gene of TYLCCNV DNAβ with the vector containing a bi-directional promoter revealed that the βC1 protein has no impact on expression of either the virion- or the complementary-sense promoter.     

交联精氨酸对阴离子染料日落黄、柠檬黄的吸附行为

研究了pH值、染料浓度、吸附时间、温度对交联精氨酸吸附能力的影响．用精氨酸作单体、戊二醛为变联剂，20℃水浴摇床150r／min反应240h，离心，洗涤，冷冻干燥，制备成交联精氨酸吸附剂．在25℃，pH2．0，吸附剂对日落黄和柠檬黄48h的吸附量分别达到408．92mg／g和306．96mg／g，吸附反应符合Langmuir等温吸附模型．结果表明：方法简单、条件温和；产物对阴离子染料日落黄和柠檬黄有良好的吸附性能．     

Obtained transgenic wheat expressing pac1 mediated by Agrobacterium is resistant against Barley yellow dwarf virus-GPV

At present, genes used to transform wheat for the virus resistance are mostly from the virus, such as coat protein gene[1,2], replicase gene[3] and movement gene[4], and transformed wheat with these genes showed resis-tance to the corresponding virus. How…     

浙贝母黄化矮缩枯萎病发生特点与防治技术研究

浙贝母黄化矮缩枯萎病是近几年危害严重的新病害.调查和试验结果表明:选用无病种贝是切实可行的高效防治方法.采用1)58%瑞毒霜.锰锌500倍,2)多菌灵200-500倍,3)75%达霜宁(百菌清)500倍药剂浸种0.5h也有一定的防治效果.     

Clone of Chinese Jinan red-cross yellow cattle and evaluation of reproductive characteristics of cloned calf

Somatic cell clone technology is a viable approach to preserving endangered livestock and wildlife genetic resources. In the present research, somatic cell nuclear transfer （SCNT） was performed using granulose cells from the critical endangered Chinese red-cross yellow cattle as donor cells. A total of 211 oocytes were manipulated and 166 （79%） of them were successfully enucleated. 112 （67.4%） SCNT embryos were reconstructed, 94 （83%） of them cleaved, and 48 （43 %） of them developed to blastocyst stage. SCNT blastocysts were transferred to 6 Holstein recipients, and 2 （33%） of them were found to be pregnant. One of them maintained to term and delivered a calf, whereas another aborted. Effect of different fusion buffer （mannitol vs. Zimmerman fusion buffer） and different activation methods （calcium ionophore＋6-DMAP vs. cycloheximide＋CB） on fusion rate and development of SCNT embryos were investigated. The results indicated that： （i） on condition of two DC pulses of 2.5 kV/cm for 10 μs each, fusion rates were higher in mannitol solution than in Zimmerman fusion buffer （71% vs. 61%, respectively, p 〈 0.05）, but the blastocysts rates did not differ between two treatments （36 % vs. 39 %, p〉0.05 ）; （ii） There was no significant difference in development rates to the blastocyst stage for SCNT embryos activated by calcium ionophore＋6-DMAP or by cycloheximide＋CB （42% vs. 46%, respectively, p〉0.05）. Microsatellite DNA analysis examining 28 loci confirmed that the cloned calf was genetically identical to the donor Jinan red-cross yellow cattle and different from the recipient females. Growth and reproductive performance of cloned cow were evaluated, and there were no difference i cross-red n it between cloned and normal control Jinan yellow cattle. Furthermore, the cloned yellow cow has delivered a healthy yellow calf.     

黄河无定河流域分布式时变增益水文模型的应用研究

黄河无定河流域是典型的黄土高原沟壑地区，水文时空变化大，观测站点有限，水文过程的非线性问题和人类活动影响问题比较突出．本文针对黄河无定河流域内不同尺度水文循环模拟问题，对作者开发的分布式时变增益水文模型进行了改进，拓展到了月、日、时三个时间尺度，应用于无定河内的大理河、小理河及岔巴沟三级不同空间尺度的流域．研究表明：分布式时变增益水文模型是水文系统非线性理论与分布式流域水文模拟结合的一种新途径，水文系统模拟的精度比较满意．该模型为分析土壤湿度的时空分布与变化以及人类活动的影响，提供了一种量化分析的有效工具．     

Transport of Yellow Sand in Stratified Flow over an Isolated Mountain Ridge

The transport of yellow sand over mountain regions in the presence of internal gravity waves has been investigated numerically. The motion of yellow sand particles has been simulated in a Lagrangian frame of reference by solving the time-dependent Reynolds averaged Navier-Stokes equations. An implicit time integration in a fitted body grid arrangement was used to simulate the stratified flow over an isolated ideally bell-shaped mountain. The transport and deposition of particles of various sizes, and of the altitudes where particles were released have been analyzed. Particular attention was given to transport patterns of different sized particles in various atmospheric conditions. The results show that the particle size and the re-lease altitude are both important factors in determining the trajectories of the particles. Small particles tend to be transported a long distance over the mountains, whereas heavier particles settle down around the re-lease source. Due to the existence of an internal gravity wave, the particle release altitude affects the trajec-tory of the particles. The analysis and results provide a very useful tool for the study of atmospheric flow and transport of pollutants over real topographies.