An enzyme immunoassay for mouse epidermal growth factor utilizing a liquid phase double-antibody system

Summary An enzyme immunoassay for mouse epidermal growth factor (EGF) involving a liquid phase double-antibody system was developed. The EGF--galactosidase conjugate prepared was stable for at least 8 months. By this method, EGF was detectable at a concentration as low as 20 pg per tube. The concentrations of EGF in various tissues of mice are also presented.This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.     

Energy flow and community structure in freshwater ecosystems

The goal of this article is twofold: 1) It aims at providing an overview on some major results obtained from energy flow studies in individuals, populations, and communities, and 2) it will also focus on major mechanisms explaining community structures. The basis for any biological community to survive and establish a certain population density is on the one hand energy fixation by primary producers together with adequate nutrient supply and the transfer of energy between trophic levels (bottom-up effect). On the other hand, predator pressures may strongly control prey population densities one or more trophic levels below (top-down effect). Other interpopulation effects include competition, chemical interactions and evolutionary genetic processes, which further interact and result in the specific structuring of any community with respect to species composition and population sizes.     

透射焦散线法研究甲板开口边缘Ⅰ型裂纹的应力强度因子

为研究船舶甲板开口边缘受拉的Ⅰ型裂纹的应力强度因子,并探索焦散线方法在船舶结构中的应用,采用透射焦散线方法进行了实验研究．实验中模拟一般散货船带舱口的上甲板,制作了带孔有机玻璃透明试件,并采用加热法开出孔边裂纹．在数字焦散线实验系统中进行实验,获得了清晰的全场焦散线数字图象．应用焦散线图象自动处理程序精确地测出焦散线的最大直径以计算应力强度因子,并对裂纹尖端初始焦散线引起的实验误差作了处理．实验结果与理论值十分接近,表明船舶甲板开口边缘受拉的Ⅰ型裂纹的应力强度因子与甲板中的总纵拉力成正比．     

人——管理信息系统中的重要因素

通过对MIS开发普遍失败的原因分析，重点说明了以往在MIS的开发和使用中人的因素所存在的问题。针对我国目前在MIS建设中忽略各种人员对MIS开发成功的影响而产生的问题，提出了应对的措施与建议。     

组织合作网与生产要素的流动

提出组织合作网的概念.由于传统的生产要素不合理,影响其流动,通过组建合作网,生产要素流动,资源重新配置,组织结构得以调整和优化,并得到创新,从而保持合作网的活力.合作网有个领头组织,由他带领使网内每个成员获利,创造出新的生产力.     

冻融条件下软岩隧道冻胀力计算分析

针对软岩体含水率高、孔隙率大、对温度变化敏感等特点,本文首先在一定假设的基础上,分析了温度影响范围内软岩体冻结后,内部水冰相变会导致隧道衬砌承受冻胀力的原因,推导出了其弹性力学解,其次分析了融化过程及融化后冻胀力变化的过程及原因,运用弹性力学复变函数法推导出了冻融后隧道衬砌所承受冻胀力的表达式,最后给出了算例并指出将来的研究方向。     

中碳钢缺口疲劳裂纹的萌生及扩展规律的研究

经研究中碳合金结构钢３５ＣｒＭｏ，４２ＣｒＭｏ在不同应力比下（Ｒ＝０．１和Ｒ＝０．３）三点弯曲缺口试件的疲劳裂纹萌生及长，短裂纹的扩展规律，确定了该钢的缺口根部最大应力范围△σｍａｘ和疲劳裂纹萌生周次Ｎｉ的关系及疲劳裂纹萌生门截坎值（△σｍａｘ）ｔｈ，并得出了这两种钢在长，短期纹阶段的扩展规律。     

Effect of hydrogen peroxide on the binding of Merocyanine 540 to human erythrocytes

The fluorescent dye Merocyanine 540 (MC540) is often used as a probe to monitor the molecular packing of phospholipids in the outer leaflet of biomembranes. In a previous study we showed that the increased staining of erythrocytes with a perturbed membrane structure was mainly due to an increase in the fluorescence yield of cell-bound MC540, rather than to an increase of the number of bound molecules. Erythrocytes and ghosts exposed to continuous fluxes of H₂O₂ exhibited pronounced lipid peroxidation. Further, red blood cells subjected to this form of oxidative stress also showed increased staining with MC540. It appeared that this was caused by a strong increase in binding of MC540, together with a slight red shift of the fluorescence emission maximum and a small increase in the fluorescence yield of bound MC540. The changed MC540 binding characteristics were not observed when lipid peroxidation was suppressed by the presence of the antioxidant BHT in the incubation medium. However, open ghosts exposed to H₂O₂ showed no increase of MC540 binding, excluding a direct involvement of lipid peroxidation. Measurement of fluorescence emission spectra and gel filtration studies showed that MC540 can bind to H₂O₂-exposed hemoglobin. Experiments with erythrocytes lysed in hypotonic medium after exposure to H₂O₂ revealed that peroxidation of lipids with H₂O₂ induced a non-specific permeabilization of the plasma membrane to MC540, thereby allowing MC540 to bind to the oxidatively denatured, more hydrophobic hemoglobin. These results indicate that conclusions about packing of phospholipids in the outer leaflet of the membrane based on increased MC540-staining should be drawn with care. Received 27 September 1996; received after revision 5 November 1996; accepted 27 November 1996     

巨型模锻水压机立柱应力在线检测及保护系统

通过对100MN多向模锻水压机的立柱在偏载工况下的受力状态的简要分析,并根据立柱应力特征与水压机的结构特点,提出了一种新型的非接触式立柱应力在线检测方法。以电涡流传感器为基本单元,并采用动态“O”点技术,建立了100MN多向模锻水压机的应力检测与保护系统及其软件。实验研究及应用表明,所研制的系统具有良好的稳定性和抗干扰能力,同时很好地抑制了零漂对检测精度的影响,该检测方法可应用于大型机械(轧机、挤压机等)封闭框架应力的间接检测。     

应力法下料的脆性断裂设计

在简要介绍裂纹技术及断裂设计概念的基础上，讨论了应力法断料的概念及特点，确定了地低应力脆断的敏感性应力状态和最佳预荷方式，以表征切口尖端附近应力场强的无量纲因子ｎ（ａ／ｂ）为基础，通过实验和数值计算得出包括切口尖端半径、切口深度和切口张角在内的适宜切口参数，从而为应力法下料的应用提供了技术保证。