Superoxide dismutase activity during the plasmodial life cycle ofPhysarum polycephalum

Summary Superoxide dismutase activity was slow throughout the cell cycle of surface cultures ofPhysarum polycephalum. This activity increased markedly when the organism was induced to spherulate. Glutathione (GSH) and hydrogen peroxide (H₂O₂) concentrations changed very little during the cell cycle. During spherulation GSH decreased; H₂O₂ and the cyanide-resistant respiration of plasmodial homogenates increased.     

关于Directly-Riemann积分收敛性判别法

利用Directly-Riemann积分的基本性质，研究了判别Directly-Riemann积分的收敛性问题。     

A simple diagnostic method for the differentiation of Tamm-Horsfall glycoproteins from healthy probands and those from recurrent calcium oxalate renal stone formers

Tamm-Horsfall glycoproteins (THPs)^* from healthy probands, and those from a majority of recurrent calcium oxalate renal stone formers, reveal different properties when analyzed using isoelectric focusing. The pl-values of THPs from healthy probands are approximately 3.5 while THPs from recurrent renal stone formers have pl-values between 4.5 and 6. The two groups of THPs exhibit completely different protein patterns in IEF. This proves the structural difference of these THPs. The differences in IEF analysis allow the differentiation between THPs from healthy probands and those from recurrent calcium oxalate stone formers. These differences could possibly by used as a simple diagnostic method for the recognition of recurrent calcium oxalate renal stone formers.     

“文字学”与“汉字学”辨析

在语言学中,“文字学”这个术语常有两种含义：一种是指“以不同民族文字为对象,揭示人类文字构成和运用的一般规律的科学”;另一种是指“研究汉字的学问”。目前,无论是从术语规范的角度出发,还是从人们现今的实际使用状况来看,后一种用法已经很不合适了,应予以剔除。另外,“研究汉字的学问”目前还有着多种异称：“文字学”、“汉字学”、“中国文字学”等,为避免术语使用的混乱,应确定学术界普遍认可的“汉字学”作为规范的正名。     

Cellular responses to mild heat stress

Since its discovery in 1962 by Ritossa, the heat shock response has been extensively studied by a number of investigators to understand the molecular mechanism underlying the cellular response to heat stress. The most well characterized heat shock response is induction of the heat shock proteins that function as molecular chaperones and exert cell cycle regulatory and anti-apoptotic activities. While most investigators have focused their studies on the toxic effects of heat stress in organisms such as severe heat stress-induced cell cycle arrest and apoptosis, the cellular response to fever-ranged mild heat stress has been rather underestimated. However, the cellular response to mild heat stress is likely to be more important in a physiological sense than that to severe heat stress because the body temperature of homeothermic animals increases by only 1–2°C during febrile diseases. Here we provide information that mild heat stress does have some beneficial role in organisms via positively regulating cell proliferation and differentiation, and immune response in mammalian cells.Received 14 May 2004; received after revision 2 August 2004; accepted 16 August 2004     

高效Ni 2+ 吸附菌株分离鉴定及特性研究

采用浓度梯度筛选的方法,从活性污泥中筛选到一株能耐受并吸附Ni 2+的菌株,命名为Ni-1.采用单因素法对菌株Ni-1的最适培养条件进行了考察,确定培养条件为t=30℃,pH=7.0,1.0%的NaCl.考察了菌株Ni-1对Cu2+和Ni 2+的吸附性能,并用表面响应法优化了吸附条件.菌株Ni-1吸附Cu2+的最优条件为Cu2+100mg/L、投加菌量(湿重)0.85g、溶液pH=6.0,在此条件下对Cu2+的最大吸附率为87.13%.菌株Ni-1吸附Ni 2+的最优条件为Ni 2+50mg/L、投加菌量(湿重)0.85g、溶液pH=6.0,在此条件下对Ni 2+的最大吸附率为84.32%.实验结果表明该细菌对较低浓度的Cu2+和Ni 2+有较好的吸附性能.     

客车气压制动时延分析及其控制研究

提出了一种客车气压制动时延控制方法.考虑到客车气压制动系统的延时特性,建立了客车气压制动回路的数学模型,运用Matlab/Simulink和AMESim软件分别建立了其仿真模型,采用理论分析与仿真实验相结合的方法,分析了客车气压制动的时延特性;基于闭环反馈控制理论,提出了客车气压制动回路时延控制方法.运用PID控制算法设计了时延控制器,并进行了对比仿真.仿真结果表明,进行时延控制后,客车的制动时延为0.3s,制动距离缩短1.8m,提高了客车的制动稳定性.     

小鼠孤雌生殖胚胎干细胞的分离及分化潜能研究

用免疫外科法从小鼠孤雌生殖胚胎分离胚胎干细胞,并研究了其在体内、体外的分化潜能．结果发现:从小鼠孤雌生殖的胚胎中可分离出胚胎干细胞(pES),可以传代培养25代,能表达很强的碱性磷酸酶,核型稳定呈40XX．培养至第18代的pES在体内可诱导肿瘤形成,并可分化为三个胚层的组织细胞．免疫组化结果显示:神经细胞特异性烯醇化酶（NSE）、肌肉特异性肌动蛋白?-actin均呈阳性,表明分离培养的pES在体内可至少分化为来自外胚层和中胚层的组织细胞．传至第20～24代的pES细胞,经体外定向诱导分化,可定向分化为节律性收缩的心肌细胞及神经细胞．免疫组化检测显示节律性收缩的心肌细胞表达?-actin,而神经细胞表达NSE．结果表明:利用免疫外科法可从孤雌生殖的小鼠胚胎建立pES,这些pES在体内、体外都具有分化为多种类型细胞的潜能．     

考虑公平偏好和产品纵向差异化的产品定价

针对双寡头市场中两竞争性的企业考虑有限理性的公平偏好时的最优定价策略问题进行了研究。这两个竞争性的企业分别销售高质量产品和低质量产品。与传统完全理性的企业决策不同的是，两企业在决策时考虑了经典的公平偏好因素。结论表明：当单个企业考虑公平偏好时，两个企业均会采取降价策略来吸引更多的消费者；此外，处于不利不公平时企业的降价幅度大于处于有利不公平时企业的降价幅度。当两个企业考虑公平偏好时，双方都会降价，且它们降价的幅度比单个企业考虑公平偏好情形时的要大。在这种情况下，高质量企业的反应降价程度比低质量企业强烈，且总的降价额度与各感知参数和心理预期市场比例有关。