Galectin-3 stabilizes heterogeneous nuclear ribonucleoprotein Q to maintain proliferation of human colon cancer cells

Comparative analysis of proteomes using 5-fluorouracil (5-FU)-resistant human colon cancer cell line revealed that decreased galectin-3 expression was significantly associated with retarded proliferation. However, in the presence of 5-FU proliferation rate of cells with suppressed galectin-3 expression did not differ from that of cells with normal galectin-3 expression, even galectin-3 suppression augmented apoptosis. Mechanism by which galectin-3 regulates cancer cell proliferation has been identified in immunoprecipitates of the anti-galectin-3 antibody. Heterogeneous nuclear ribonucleoprotein Q (hnRNP Q) was identified as a protein interacting with galectin-3. Interestingly, while galectin-3 protein was not affected by the hnRNP Q level, its suppression was accompanied by a decrease in hnRNP Q expression. The present study demonstrates that galectin-3 stabilizes hnRNP Q via complex formation, and reduction in the hnRNP Q level leads to slow proliferation and less susceptibility to 5-FU. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. B.C.Yoo; S-H.Hong; These two authors contributed equally to this work. Received 10 September 2008; received after revision 19 October 2008; accepted 07 November 2008     

随机细胞人工神经网络的稳定性

基于细胞神经网络输出函数饱和线性特性,采用分割状态空间为若干子区域的方法,对处于噪声环境下的细胞神经网络的几乎必然指数稳定性进行了分析.在噪声扰动项分别满足Lipschitz条件和线性条件下,依据平衡点所处子区域位置,得到了对应区域中仅依赖于网络参数构成的矩阵负定性的若干代数判别条件.特别地,如果平衡点为区域内点,所得结果是非常方便检验的.研究结果可供网络设计参考,并可用来计算网络抗干扰强度.     

开关磁阻电机电动轮驱动系统

基于汽车动力学的分析,提出了电动轮驱动系统应遵循的控制原则即输出转矩闭环控制;比较了目前常用的开关磁阻电机转矩控制策略,并提出了新的控制方案.该方案通过放置辅助检测绕组和采用硬件积分器,对电机绕组磁链进行实时检测;结合绕组电流进行转矩估算,经过转矩误差PID反馈控制,实现电机输出转矩对转矩给定指令的跟踪.建立了实际的转矩闭环控制系统并进行了实验研究.实测得到的电机输出特性证明了所提出的控制策略的正确性.     

VISA存储网络系统元数据管理的研究

为了提高虚拟接口存储结构(VISA)存储网络系统的性能,设计实现了基于块级的元数据管理子系统.该系统的元数据管理子系统实现了物理设备和逻辑设备的块地址空间之间的映射,支持动态地址映射、动态块重分配和热块冗余技术,可以对I/O请求的路径和数据传输的路径完全控制,从而有效地改善了VISA存储网络系统的I/O性能,使数据布局更加合理,提高了存储管理的灵活性,初步实现了负载均衡,并且使VISA存储网络系统具备了容错能力和节点的动态增加能力.测试结果表明:在加入了基于块级的元数据管理功能带来相应开销的情况下,VISA存储网络系统原型与传统存储网络系统iSCSI Linux MD相比,传输延迟减少了8.2%～21.0%,传输吞吐率提高了8.7%～11.9%.     

光学编码成像术中光源稳定性研究

针对光学编码成像的光源强度随时间波动对编码成像结果的影响,研究了编码机制对光源光强变化响应的表现,发现了阿达玛变换光学系统对光源强度波动的特异敏感性规律:像元强度改变量与光源光强波动量成比例;一个编码周期内光源光强的多次波动导致像元强度改变量按次累加但并不导致发散;各次源强度波动对像场强度改变量的分布具有循环性.采用数值模拟方法计算了与1×7阵列像场对应的7阶S矩阵编码成像,结果表明光源光强随时间的变化对编码成像质量有严重影响.指出了在编码成像系统中所用的光源的不稳定性应该受到的限制.     

不确定奇异系统的有限时间容错控制器设计

讨论了不确定线性奇异系统的有限时间容错控制问题.针对一类含有时变、范数有界参数不确定奇异系统,运用线性矩阵不等式方法(LMI),设计了鲁棒容错状态反馈控制律,使得当奇异系统执行器发生故障时,故障闭环系统仍然是正则、无脉冲,且保持有限时间状态稳定,给出了有限时间容错控制器存在的充分条件和设计方法,该方法通过解线性矩阵不等式即可得到容错控制器的设计结果.数值算例验证了该容错控制设计方法的有效性.     

峰值电流升压型PWM变换器内部补偿网络设计

分析了峰值电流升压型PWM变换器环路中极零点补偿对稳定性的影响.根据补偿原则,采用电容等效乘法电路,设计了一种应用于峰值电流升压型PWM变换器的内部补偿网络.利用模拟乘法电路的等效倍增功能,实现了补偿电容的最小化,使内部补偿成为可能.仿真结果表明:该补偿网络使变换器具有高稳定性,补偿后,输出电压的波纹很小,输出纹波电压仅在±8 mV范围内抖动,满足电压稳定输出的性能要求.     

一个混沌电路的自适应同步

针对从动系统对主动的参数未知的情形,讨论了一个混沌电路的同步控制问题.当从动系统对主动系统的参数未知时,采用李亚普洛夫稳定性理论和自适应设计方法,设计出了一个自适应控制器.该控制器可实现主从混沌系统间的自适应同步并同时实现参数的辨识.仿真结果表明该设计方法的有效性.     

一类随机非线性系统的输出反馈全局镇定

将基于增益观测器的非线性系统输出反馈全局镇定控制器设计方法推广到随机情形,使之适用于一般的随机非线性系统.与已有基于反步法设计的镇定控制器相比,该方法不要求该随机非线性系统满足严格反馈条件,对其噪声向量函数也不要求满足光滑性条件.基于李亚普诺夫第二方法,讨论了所设计的控制器稳定的条件.一个二阶仿真算例表明该控制器设计方法是有效的.     

Evidence for the intracellular accumulation of anandamide in adiposomes

Anandamide is a lipid messenger that carries out a wide variety of biological functions. It has been suggested that anandamide accumulation involves binding to a saturable cellular component. To identify the structure(s) involved in this process, we analyzed the intracellular distribution of both biotinylated and radiolabeled anandamide, providing direct evidence that lipid droplets, also known as adiposomes, constitute a dynamic reservoir for the sequestration of anandamide. In addition, confocal microscopy and biochemical studies revealed that the anandamide-hydrolase is also spatially associated with lipid droplets, and that cells with a larger adiposome compartment have an enhanced catabolism of anandamide. Overall, these findings suggest that adiposomes may have a critical role in accumulating anandamide, possibly by connecting plasma membrane to internal organelles along the metabolic route of this endocannabinoid. S. Oddi, F. Fezza: These authors contributed equally to the study.