东京野茉莉雌雄蕊的形态及显微结构

【目的】了解东京野茉莉(Styrax tonkinensis)花部及雌雄蕊的形态及内部显微结构,探寻其花粉败育原因。【方法】运用光镜和电镜技术,对其雌雄蕊内外部形态特征、显微结构和超显微结构进行观察研究。【结果】①每朵两性花中有雄蕊10枚,由花丝和花药组成。花丝宽(0.38±0.12)mm,长(8.24±0.72)mm,成熟花药由2个狭长圆形的药室组成。成熟花粉在扫描电镜下可见3或4个萌发沟,外壁有细网状纹饰,还发现有形态皱缩发育不良的花粉。透射电镜显示发育良好的花粉壁有2层,细胞内质浓厚,含有高尔基体、较多的线粒体、高电子致密物质和淀粉粒等结构。② 雌蕊1枚,由柱头、花柱和子房组成。柱头上有“Y”形裂沟和椭球状乳突,传粉后,柱头上有分泌物。花柱中空。子房3室,多枚倒生胚珠; 发育后期,只有1～2个发育为种子,其余退化。【结论】东京野茉莉花部特征符合安息香科特征,花粉粒3种形态; 柱头湿型,胚珠倒生; 可育花粉粒和珠被细胞内细胞质浓,细胞器较多。     

Investigation of structural change of purple membrane in storage by transmission electron microscope and atomic force microscope

The structural change of purple membrane during storage has been investigated by means of transmis sion electron microscope and atomic force microscope. It is found that many liposomes have spontaneously evolved from the purple membrane sheets isolated three years ago. The membrane proteins on the liposomes, bacteriorhodopsin, are still presented as trimers in 2-D hexagonal structure, which is the same as that in natural cell membrane. However, the cytoplasmic surface of purple membrane faced outside on the liposomes.     

中心回线瞬变电磁自适应正则化反演

采用自适应正则化反演,直接对瞬变电磁感应电动势曲线进行反演拟合。相对于传统的求取视电阻率进行定性或半定量的解释方法,利用自适应正则化反演技术可更为准确的获得地电断面的电阻率分布。通过理论模型试算表明自适应正则化反演算法收敛快速、拟合效果好,一般只需迭代5次反演就趋于稳定,迭代10次后目标函数即可达到5×10-2。对某煤矿区的中心回线装置瞬变电磁资料进行反演,和已知资料吻合较好,验证了自适应正则化反演算法可以很好的应用于瞬变电磁资料解释中。     

Application of FLUPD-DD-PCR to the study of mRNA expression of glioma cells cultured under the condition of serum starvation

Fluorescently labeled universal primer directed differential display polymerase chain reaction technique (FLUPD-DD-PCR), an improved DD-PCR, is reported for the study of mRNA expression of glioma cells cultured with the serum starvation. The fluorescently labeled universal primer (FLUP) facilitates analysis of PCR products on an auto-sequencer. Compared with the traditional DD-PCR, FLUPD-DD-PCR has advantages of separation of target bands and measurement of mRNA expression quantitatively by adopting Cy5-labeled universal primer. And then the silver staining method has been used to recover the bands for use as template in re-pcr. In this study, 4 differential ESTs, of which 3 are novel ESTs, have been obtained. This work indicates some special novel genes, which can be induced to express under serum absent condition, are involved in coping with serum starvation stress on glioma cells growth.     

青海玉树多目涡虫咽部组织结构的显微观察

利用连续石蜡切片(包括水平切、矢状切和横切)技术,H.E染色和光镜观察的方法,对青海玉树多目涡虫咽部的组织结构进行了初步的研究,结果表明:咽壁包括三层结构,外层由单层柱状上皮构成,似合胞体,上皮细胞游离面可见三层嗜酸性带状结构,其中,外层带呈刷状;咽壁的中层分为内侧发达的环肌和外侧较薄的纵肌两部分;邻接咽腔的内层上皮在游离面呈指状突起,未见上皮细胞间界限.     

青海玉树多目涡虫精巢组织结构的显微观察

利用连续石蜡切片(水平切、矢状切、横切)及H.E染色方法,对青海玉树多目涡虫精巢的组织结构进行了初步研究.结果表明:在光学显微镜下,多目涡虫精巢的组织结构与哺乳类睾丸中曲细精管的结构类似,但也有一些差别.提示:精巢的组织结构在动物的系统演化过程中是比较保守的,通过对不同进化类群动物精巢的组织结构进行比较研究,也许有助于对动物系统演化的探究.     

高稳定度电磁透镜电流源基准电阻的温度补偿

电流源的稳定度是影响高稳定度电磁透镜分辨率与成像质量的关键因素之一,而基准电阻作为电流源的反馈元件,其稳定度直接影响电流源的稳定度。为了提高基准电阻的稳定性,通过分析基准电阻与页温度系数(negative temperature coefficient, NTC)热敏电阻的温度特性,并提出了一种通过NTC热敏电阻对基准电阻进行温度补偿的电路结构。最后以Pt100电阻作为基准电阻,分析了该电路结构的性能。结果表明,与基准电阻相比,经补偿后所得到的等效基准电阻的稳定性提高了10倍以上。     

聚丙烯酰胺凝胶电泳快速显色方法的研究

通过对聚丙烯酰胺凝胶电泳的固定方法、时间、染色液种类、染色方法及蛋白灵敏度的研究,确定了以10%三氯乙酸为固定液,室温固定10min的固定方法;染色液为：考马斯亮蓝G-250、95%乙醇和10%磷酸,染色时间为15min;蛋白质灵敏度与考马斯亮蓝R-250相当,达到0.1μg。     

甘草酸二钾护理液皮肤修复作用的研究

目的研究甘草酸二钾护理液及含甘草酸二钾卫生巾对擦伤皮肤的修复作用。方法用SD大鼠,制造皮肤擦伤模型,将动物随机分为阳性对照组、阴性对照组、甘草酸二钾护理液高、中、低剂量组、甘草酸二钾卫生巾组,一定时间后,检测创面愈合率,通过HE染色观察损伤皮肤组织病理学变化。结果造模后第7 d和第14 d护理液高、中剂量组和卫生巾组大鼠的皮肤愈合率和组织学评分与阴性组比较具有统计学意义(P 0.05)。结论甘草酸二钾护理液及含甘草酸二钾卫生巾具有促进皮肤修复的作用。     

Effect of Si on austenite stabilization,martensite morphology,and magnetic properties in Fe-26%Ni-x%Si alloys

The effect of Si on the austenite stabilization, martensite morphology, and magnetic properties in Fe-26%Ni-x%Si (x=3.5, 5, and 6) alloys have been studied by means of transmission electron microscopy (TEM) and M?ssbauer spectroscopy techniques. TEM observations reveal that the martensite morphology is closely dependent on the Si content. The volume fraction changes of martensite and austenite phases, the hyperfine magnetic field, and isomer shift values have been determined by Mssbauer spectroscopy. The M?ssbauer study reveals that the hyperfine magnetic field, the isomer shift values and the volume fraction of martensite decrease with increasing Si content.