主轴径向回转误差测试中的误差分析

对以圆柱体作为被测试对象在主轴径向回转误差的测试中可能引入的测量误差进行了分析。讨论了主轴安装倾斜或偏心引入的测量误差、测头安装偏心引起的测量误差、以及测量仪器引起的线性漂移等误差项的来源及对实际误差的精度影响，并给出了误差表达公式。指出精密主轴的偏心运动和安装测头的装夹偏心有相关性，实际分离的偏心值为两者的耦合项；偏心运动与主轴和被测试对象材料存在相关性；由主轴轮廓外形引入的测量误差在满足精度的前提下，可以使用标准圆柱体作被测试对象。     

锭子弹性支承刚度对其不平衡响应的影响

将锭子分解为锭杆和支承两个子结构，采用传递矩阵法导出锭杆的不平衡响应动力学方程，运用动态子结构方法分析计算了一种双弹性支承纺纱锭子的不平衡响应，作出了用于锭子弹性支承优化设计的锭子的不平衡响应与支承动刚度参数关系曲面。     

加工孔用多轴头坐标的计算

分析了多轴头的坐标计算方法，通过编制计算程序，减少了计算的工作量，而且确保了计算的准确性和精度。     

超重力反应器中碳化反应参数对碳酸钙产品的影响

结合碳酸钙的成核、生长动力学论述了超重力反应结晶法制备纺锤形碳酸钙过程中主要工艺参数对碳化过程的目标函数之一产品碳酸钙的沉降比体积值的影响,并提出必要的控制条件.同时,将超重力下合成的纺锤形碳酸钙与传统制备方法制得的纺锤形碳酸钙进行了比较,结果表明,其质量要优于传统塔式设备制备的纺锤形碳酸钙.     

超细纺锤形CaCO3制备过程研究

在超重力反应器中,Ca(OH)2经碳化反应制得超细纺锤形碳酸钙.采用pH计、XRD、TEM、EDTA络合滴定原位检测了超重力反应沉淀法制备轻质纺锤形碳酸钙的碳化过程特性.结果发现:碳化反应前期,二氧化碳吸收为控制步骤,碳化反应后期转化为Ca(OH)2溶解控制.纺锤形碳酸钙的形成无论是形貌还是晶型都是一个渐变的过程.     

蛋白体外结合实验联合质谱分析鉴定 Num1 的互作蛋白

目的: 为了解析芽殖酵母 Num1 蛋白在纺锤体定位和线粒体中的调节机制．方法: 利用大肠杆菌原核表达 并纯化了在 Num1 功能中起核心作用的补丁组装(PA) 结构域的重组蛋白, 通过蛋白体外结合实验(Pull-down) 分 离了酵母细胞中与 PA 结构域相结合的蛋白复合物,并对其进行了质谱分析．结果: 鉴定了一系列新的 Num1 互作蛋 白,包括核糖体蛋白,参与蛋白折叠、分配及转运的内质网和高尔基复合体蛋白, 参与基因转录和翻译的核酸酶和 蛋白酶,及其他功能的蛋白．结论: Num1 的互作蛋白的鉴定为进一步研究 Num1 的作用机制奠定了基础.     

Mitotic regulation of the anaphase-promoting complex

Orderly progression through mitosis is regulated by the anaphase-promoting complex/cyclosome (APC/C), a large multiprotein E3 ubiquitin ligase that targets key mitotic regulators for destruction by the proteasome. APC/C has two activating subunits, Cdc20 and Cdh1. The well-established view is that Cdc20 activates APC/C from the onset of mitosis through the metaphase-anaphase transition, and that Cdh1 does so from anaphase through G1. Recent work, however, indicates that Cdh1 also activates APC/C in early mitosis and that this APC/C pool targets the anaphase inhibitor securin. To prevent premature degradation of securin, the nuclear transport factors Nup98 and Rae1 associate with APC/C^Cdh1-securin complexes. In late metaphase, when all kinetochores are attached to spindle microtubules and the spindle assembly checkpoint is satisfied, Nup98 and Rae1 are released from these complexes, thereby allowing for prompt ubiquitination of securin by APC/C^Cdh1. This, and other mechanisms by which the catalytic activity of APC/C is tightly regulated to ensure proper timing of degradation of each of its mitotic substrates, are highlighted. Received 8 October 2006; received after revision 24 November 2006; accepted 8 January 2007     

卷取机卷简部件及其芯轴的制造工艺研究

卷取机用途广泛,卷筒则是热轧卷取机的核心部件,从卷筒部件结构、工作原理和主要技术要求入手,深入开展了卷筒部件的制造工艺研究,重点分析了核心零件--芯轴的结构及加工工艺,对卷筒部件的卷曲圆采用“先组装后加工”的整体加工方案及芯轴加工采取“利用中心孔,配中心堵”的技术措施,卷简部件及其芯轴制造获得一次成功。     

Protein kinase TTK interacts and co-localizes with CENP-E to the kinetochore of human cells

Spindle checkpoint is an important biochemical signaling cascade during mitosis which monitors the fidelity of chromosome segregation, and is mediated by protein kinases Mps1 and Bub1/BubR1. Our recent studies show that kinesin-related motor protein CENP-E interacts with BubR1 and participates in spindle checkpoint signaling. To elucidate the molecular mechanisms underlying spindle checkpoint signaling, we carried out proteomic dissection of human cell kinetochore and revealed protein kinase TTK, human homologue of yeast Mps1. Our studies show that TTK is localized to the kinetochore of human cells, and interacts with CENP-E, suggesting that TTK may play an important role in chromosome segregation during mitosis.     

纺纱锭子临界转速的优化设置

先对纺纱锭子的动力特性作敏度分析，合理选择设计变量，再进行临界转速优化设置的送代计算，可使设计的纺纱锭子在工作转速区内有较小的振动和功耗。