火花光谱法测定粉末样品中的高含量硅

利用火花光谱法定量检测了硅铋样品中质量分数为３０％的元素硅，分析结果表明，相对误差不大于８．４％，相对标准偏差为５．９％，方法是简便可行的。     

硫脲介质火焰原子吸收光谱法测定铅精矿中的金

介绍了用硫酸钠消除铅的干扰，在王水－硫脲介质中用火焰原子吸收光测定铅精矿中金新方法，试验结果表明：线性范围为０－５．０μｇ／ｍＬ，方法回收率为９５．６％－１０２．１％，相对标准偏差小于２．１％。     

四极质谱仪微机控制和数据处理系统

本文在分析微机化四极质谱仪现状的基础上,以国产ZLS-150型四极质谱仪为原型,进行了四极质谱仪微机控制和数据处理系统的研制,提出了最小二乘质量标定方法。通过对质谱仪扫描规律的详细研究,探讨了新颖的定点扫描方式,较好地解决了监测过程中监测频率和监测组分数之间的矛盾。该系统经联机调试取得了满意效果。     

薄膜定量分析中的Auger线型分析方法

采用Ａｕｇｅｒ线型分析方法－因子分析方法和已知组元强度的定量解谱技术，对电子束辅助Ｎ离子注入Ｓｉ和磁控溅射方法制备的ＴｉＮｘ薄膜的高分辨率俄歇电子能谱（ＡＥＳ），进行了定量分析。讨论了化学成分对ＡＥＳ线型的影响及线型分析技术的有效性和应用范围。     

Metabonomic analysis of hepatitis B virus-induced liver failure： identification of potential diagnostic biomarkers by fuzzy support vector machine

Hepatitis B virus （HBV）-induced liver failure is an emergent liver disease leading to high mortality. The severity of liver failure may be reflected by the profile of some metabolites. This study assessed the potential of using metabolites as biomarkers for liver failure by identifying metabolites with good discriminative performance for its phenotype. The serum samples from 24 HBV-indueed liver failure patients and 23 healthy volunteers were collected and analyzed by gas chromatography-mass spectrometry （GC-MS） to generate metabolite profiles. The 24 patients were further grouped into two classes according to the severity of liver failure. Twenty-five eommensal peaks in all metabolite profiles were extracted, and the relative area values of these peaks were used as features for each sample. Three algorithms, F-test, k-nearest neighbor （KNN） and fuzzy support vector machine （FSVM） combined with exhaustive search （ES）, were employed to identify a subset of metabolites （biomarkers） that best predict liver failure. Based on the achieved experimental dataset, 93.62% predictive accuracy by 6 features was selected with FSVM-ES and three key metabolites, glyeerie acid, cis-aeonitie acid and citric acid, are identified as potential diagnostic biomarkers.     

Determination of risperidone in human plasma by HPLC-MS/MS and its application to a pharmacokinetic study in Chinese volunteers

This study presents a rapid, specific and sensitive liquid chromatography/tandem mass spectrometry （LC-MS/MS） assay for determination of risperidone （RIS） in human serum using paroxetine as an internal standard （IS）. An Alltima-C18 column （2.1 mm×100 mm, 3 μm） and a mobile phase consisting of 0.1% formic acid-acetonitrile （40：60, v/v） were used for separation. The analysis was performed by selected reaction monitoring （SRM） method, and the peak area of the m/z 411.3→191.1 transition for RIS was measured versus that of the m/z 330.1→192.1 transition for IS to generate the standard curves. The assay linearity of RIS was confirmed over the range 0.25-50.00 ng/ml and the limit of quantitation was 0.05 ng/ml. The linear range corresponds well with the serum concentrations of the analytes obtained in clinical pharmacokinetic studies. Intraday and interday relative standard deviations were 1.85%-9.09% and 1.56%-4.38%, respectively. The recovery of RIS from serum was in the range of 70.20%-84.50%. The method was successfully applied to investigate the bioequivalence between two kinds of tablets （test versus reference products） in 18 healthy male Chinese volunteers. The result suggests that two formulations are bioequivalent.     

火焰原子吸收法测定粮食中锌

本文采用火焰原子吸收法直接测定粮食中的锌含量 .方法简单、快速、准确 .结果令人满意     

Characterization of impurities in the bulk drug lisinopril by liquid chromatography/ion trap spectrometry

Two trace impurities in the bulk drug lisinopril were detected by means of high-performance liquid chromatography coupled with mass spectrometry （HPLC/MS） with a simple and sensitive method suitable for HPLC/MSn analysis. The fragmentation behavior of lisinopfil and the impurities was investigated, and two unknown impurities were elucidated as 2-（6-amino- l-（l-carboxyethylamino）- l-oxohexan-2-ylamino）-4-phenylbutanoic acid and 6-amino-2-（l-carboxy-3-phenylpropylamino）-hexanoic acid on the basis of the multi-stage mass spectrometry and exact mass evidence, The proposed structures of the two unknown impurities were further confirmed by nuclear magnetic resonance （NMR） experiments after preparative isolation.     

二阶导数光谱法测定蛋白质的含量

导数光谱是紫外可见分光光度法中用以排除干扰的一种技术。本文用日立220—A紫外可见分光光度计中测定了血清蛋白质的含量。通过实验得出的线性回归方程为D=155.24c—0.346;相关系数r=0.9998,样本平均回收率为100.05％,精度试验d=0.077,S=0.418,dx=0.027,Sx=0.148。此法重现好,变异系数(CV)<±1％,此法简单,快速,可用于血清蛋白质的测定。     

山苍子油毛细管气相色谱分离及成分的色/质谱鉴定

首次采用毛细管色谱及色/质联用技术对云南山苍子油进行分离及成分直接定性,并提出了对其主成分柠檬醛的一种恒温毛细管色谱定量方法。