Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR

The outbreak of a novel influenza A （H1N1） virus across the globe poses a threat to human health. It is of paramount importance to develop a rapid, reliable and inexpensive diagnostic procedure. Based on the bioinformatic information from public database, primers specific for influenza A virus surface protein haemagglutinin （HA） of several subtypes （including H1, H2, H3, H5, H7 and H9） were designed. Primer-specific PCR products were subiected to sequencing for accurately distinguishing H1 and H3 subtypes from others. This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1, but also provide the strategies to monitor other new types of influenza virus with explosive potential.     

高原山区道路坡度对机动车比功率和气态排放物的影响

为研究高原环境下道路坡度对车辆比功率（Vehicle specific power ,VSP）、NOx和CO2的影响,利用车载排放测试系统对轻型柴油车进行实际道路试验。试验结果表明：忽略坡度对VSP的数值大小造成差异,引起VSP分布趋势变化。在坡度10 %范围内,含坡度与忽略坡度的VSP差值和坡度具有较强相关性。当道路坡度绝对值小于1时,可以忽略坡度计算VSP值。忽略坡度的VSP对划Bin区间造成聚集,扰乱了Bin区间与排放相关性;忽略坡度Bin区间排放值更接近于平均排放值。含坡度计算的VSP值与NOx、CO2排放速率有较好的相关性。     

兔子和小鼠不同组织苹果酸脱氢酶活性的研究

本文采用苹果酸脱氢酶(M DH)催化过程中辅酶Ⅰ氧化还原型转化的特征,测定了兔子和小鼠不同组织M DH的比活力和米氏常数(km),比活力在370~1 055单位/m g蛋白之间,km范围为3.57~6.49×1-0 5m o l/L.快速老化小鼠病理模型的km增大10倍,为1.49~5.64×10-4m o l/L.     

THE INFLUENCE OF DRAWING CONDITIONS OF PET POY ON STRUCTURE AND PROPERTY OF THE FIBER

Drawing tests upon PET POY have been made by using dynamic thermal stress analyser,which show that the dynamic thermal stress of fiber is readily affected by drawing conditions. Aseries of samples have been obtained under different drawing velocities, drawing temperatures anddraw ratios. The variations of structural factors of fiber such as the orientation, crystallinity,crystallite size and the thermal shrinkage have been measured. It is proved through experimentsthat the thermal shrinkage(Y) of the drawn fiber of PET POY is influenced by the orientation (rep-resented by sonic velocity x_1) and crystallinity (x_2) of the fiber. The quantitative relationship is es-tablished using the binary regression method: Y=10.2246+2.2030x_1-26.2670x_2 The mechanical properties such as tenacity, elongation, yield stress, initial modulus and the te-nacity at 10% elongation, etc. have also been measured. The relations between the tenacity at 10%elongation (D) and drawing velocity(V), draw ratio (DR), drawing temperature (T), and sonic ve-locity and crystallinity have been obtained using a method of mathematical statistics: D=4.594-0.009 6V , D=-8.937+7.170DR, D=2.866+0.00629T, D=-7.34+2.866x_1+6.314x_2 These equations fit well with data from the experiments. This work will be benefical to the control of quality of products and the development of newproducts.     

单圆弧盘型凸轮机构特性的比较研究

提出了用高副低代的化归方法研究单圆弧对心直动滚子从动件盘型凸轮机构的特性．论证了化归后的机构瞬时速度、加速度和压力角与原机构等价，在此基础上讨论了原机构的运动特性．并以最大无量纲速度和加速度为标准，将凸轮机构从动件常用运动规律特性与该圆弧凸轮的从动件运动规律特性加以比较，指出其适用场合为中、高速轻载。     

我国U15男子篮球运动员“手感”与“步法”技术能力的发展现状及成因

为探究我国U15男子篮球运动员的手感与步法技术能力的发展现状,运用文献资料、数理统计、测试和访谈等研究方法,对2012~2016年参加全国U15男子篮球比赛运动员的专项技术成绩数据进行整理和解析,并依照时间顺序对各批次运动员完成各项技术的状况进行纵向动态比较,且对成因进行深入剖析.据此,对我国青少年篮球运动员在专项技术能力培养方面提出加强考核内容及标准的灵活性和注重训练内容的均衡性等建议,力求为我国男子篮球竞技后备人才的可持续性发展提供有益参考.     

接触爆炸和近距离爆炸比冲量数值仿真研究

对于接触爆炸和近距离爆炸的情况,炸药形状、起爆点位置和产物喷射均会造成载荷空间分布复杂,给理论分析和工程应用带来困难。分别采用流固耦合方法和PBM方法对Nansteel近距离爆炸子弹抛掷实验进行数值仿真,结果表明PBM模型仿真结果和实验结果吻合较好,而流固耦合模型仿真结果仅为实验值的70%左右.对于高度为3～10 cm的等长径比TNT药柱接触爆炸仿真结果表明,中心点比冲量随药柱高度线性变化.对640 g和1 250 g等长径比TNT药柱仿真结果表明,爆心距的变化主要影响靶板中心点6 cm半径内的比冲量,基于量纲分析推导出了比冲量的经验公式.     

用煤系高岭土制取白炭黑的研究

介绍了以煤系高岭土为原料制备白炭黑的新方法。详细考察了高岭土活化温度、酸度、酸量、反应时间等对白炭黑物化参数的影响,并分别用X射线衍射、扫描电子显微镜、激光粒度仪及N2静态容量吸附法等物理手段测试了白炭黑的物相结构、表观形貌、二氧化硅含量、粒度及比表面积和孔结构。结果表明,用该方法制得的白炭黑比表面和纯度远远高于国家标准,并且中孔孔隙结构发育,粒度分布集中。     

椰壳渣制备高比表面积活性炭

通过正交实验和单因素实验探讨了以椰壳渣为原料、KOH为活化剂制备高比表面积活性炭的最佳工艺条件.考查了炭化温度、活化温度、活化时间、活化剂料比等因素对实验结果的影响.在炭化温度为600℃、碱炭质量比为2∶1、活化温度为900℃、活化时间为90 m in条件下,制备出以微孔为主、比表面积达2 180 m2.g-1、总孔容为1.19 mL.g-1的高比表面积活性炭.     

HDPE膜的力学特性受损伤影响初步研究

为分析某一现代卫生填埋场边坡上铺设的高密度聚乙烯膜(HDPE膜)因施工损伤而引发的断裂机制,开展了条带拉伸试验和土工离心模型试验．拉伸试验测试了无损膜和4种情形损伤膜试样,发现损伤HDPE膜受力达到峰值强度并被拉长至一定程度后,其拉伸变形不再在整个试样长度范围内均匀发生,而主要集中在受损部位发生,故实测到的损伤膜极限伸长率仅为无损情形时的1／10左右．这一影响实际上改变了HDPE膜的破坏机制,使其由原先的渐进性破坏变成受损后的突发性破坏．两组初步离心模型试验结果发现,在轻质高压缩性的模型MSW作用下,模型膜上的损伤明显扩大,受损部位的拉伸变形十分明显并接近断裂,表明损伤对膜的实际工作性状有很大影响．