脂质过氧化机理及测定方法

本文对有关脂质过氧化方面的文献进行了综述,得出脂质过氧化过程的化学反应图式。讨论了多种测定脂质过氧化的方法。     

在盐酸酸化卤水中钛的防护方法研究(续)

2 阳极保护的研究 2.1实验 2.1.1材料和试样 用牌号为TA2的锻压工业纯钛棒。经切割、机加工,最终获得中心有一深为8mm,直径为5mm螺纹孔的直径25mm×25mm圆柱状试样(图8)。中心螺纹孔旋入螺栓,以便电联接。此钛棒由宝鸡稀有金属加工厂提供,其化学成分如下:     

肿瘤病人红细胞膜唾液酸和岩藻糖含量的研究

本文测定健康人66例,胃癌、肺癌、肝癌病人共64例的红细胞膜唾液酸和岩藻糖含量。发现健康人上述两项指标没有性别和年龄的差异。与健康组比较,各癌症组红细胞膜唾液酸显著增多(p<0.001),而岩藻糖显著减少(p<0.001),但含量较分散、与健康者重叠较大。红细胞膜唾液酸与岩藻糖之比、各癌症组都高于健康组(p<0.001);以高于健康组M+2SD 者为阳性,则胃癌组、肺癌组和肝癌组的阳性率分别为79.2％,95.0％和80.0％,对肿瘤理论研究和临床诊断颇有价值。     

透明质酸的发酵调控研究

采用马链球菌 (Streptococcusequi)进行发酵生产透明质酸 (hyaluronicacid ,HA) .5L发酵罐发酵研究表明 :发酵调控对HA的产量和分子量有较大的影响 ,采用 2 0 g·L- 1的初始葡萄糖浓度及维持培养过程 2 0 g·L- 1的糖浓度 ,补加 5 g·L- 1的硫铵 ,采用一定的 pH控制策略可使HA的产量达到 1.0 g·L- 1,分子量在 2 .84× 10 6 Da以上 .     

丙酮酸、乳酸对体外肌酸激酶活性的抑制作用

 应用酶活测定的方法分析了肌酸激酶在不同浓度的乳酸、丙酮酸中的活性变化.实验结果说明在失活过程中没有蛋白质的聚沉,失活与乳酸浓度、丙酮酸的浓度呈正相关.通过对比实验结果提示,丙酮酸离子、乳酸离子对肌酸激酶没有明显影响,丙酮酸、乳酸是通过其解离状态的H⁺改变了机体pH值而影响肌酸激酶活性的,作者就丙酮酸、乳酸对肌酸激酶影响及其意义进行探讨.     

Releasable GABA in tubular epithelium of rat kidney

Summary The distribution of gamma-aminobutyric acid (GABA) in the rat kidney was examined by immunocytochemical techniques. GABA-like immunoreactivity (GABA-LI) was predominantly confined to the renal tubules, including the ascending parts of the distal tubules, and the loops of Henle, the collecting tubules and ducts, and the connective parts of the convoluted tubules. In GABA-positive cortical tubules, about half of the epithelial cells were labelled. The labelled cell type showed the ultrastructural features of principal cells. Depolarizing stimulation by ouabain and high K⁺ concentration evoked the efflux of endogenous GABA from kidney slices. The present findings, along with previous results, suggest that GABA released from renal tubular epithelium, and transported with the urine, might be involved in the modulation of contractility in the urinary tract.     

Free amino acid composition of the intestinal contents,intestinal cells and hemolymph ofPhilosamia cynthia larvae

Summary Free amino acid composition of the intestinal contents, intestinal cells and hemolymph has been determined in larvae of the mothPhilosamia cynthia. From the hemolymph/lumen concentration ratio, an active transport could be inferred for neutral and basic amino acids. The values of cell/lumen and hemolymph/cell ratios suggested that the active step in the transport mechanism could be localized at the luminal pole of the enterocyte for neutral amino acids (except aromatic amino acids) and at the basolateral pole of the enterocyte for basic amino acids (except arginine).This work was supported by grants from Italian Consiglio Nazionale delle Ricerche and from Ministero della Pubblica Istruzione, Rome. The authors are indebted to Prof. V. Capraro for helpful discussion.     

发酵草莓汁酸豆奶的研制

以大豆为原料，添加草霉汁，经乳酸菌发酵制作发酵草霉汁豆奶，研究了草莓汁的添加量等因素对产品质量的影响，并确定最适配方及工艺。     

鸭梨授粉前后子房核酸和蛋白质代谢的变化

本文以微量分析法对鸭梨授粉前后子房中核酸、蛋白质、核酸降解酶、蛋白水解酶的动态进行了测定分析。结果显示，花后８日内经授粉的子房中的ＤＮＡ、ＲＮＡ含量、蛋白质含量明显高于未授粉子房；核酸降解酶、水解蛋白酶的活性显著增强。表明授粉、受精作用引起子房中核酸，慢白质的旺盛合成，以及活跃的转录，翻译过程。