Intercellular dye-coupling in intestinal smooth muscle. Are gap junctions required for intercellular coupling?

Summary The dye Lucifer Yellow was injected into single smooth muscle cells in the guinea pig small intestine in order to study intercellular coupling. Dye-coupling was observed in both the circular and longitudinal muscle layers and was markedly reduced when the intercellular pH was lowered. These results suggest the presence of gap junctions among intestinal muscle cells, but are inconsistent with previous ultrastructural studies that failed to demonstrate such junctions in the longitudinal muscle.     

任意大初值等温相对论欧拉方程组的奇性形成问题

主要证明了当初始值受到挤压以及初始流体向外流出时等温相对论欧拉方程组光滑解的奇性形成问题.通过引入与解有关的泛函,证明该泛函满足适当的微分不等式,同时证明该微分不等式的解会发生奇性,继而得到等温相对论欧拉方程组解的奇性形成结果.     

基于ESTAR模型的单位根检验——Wald统计量的研究

已有的基于ESTAR模型的单位根检验通常假设α=0,但实践研究表明α显著。对α≠0的情况进行单位根检验,研究该情形下的Wald检验统计量。从理论上推导了Wald检验统计量的极限分布,并通过Monte Carlo随机模拟,结果表明该统计量有效。     

新型高精度乘用轮胎成型机的研制

为提高乘用车轮胎胎胚的精度,设计新型高精度乘用轮胎成型机结构和设备组成.在一段成型时创新设计了旋转式开合成型鼓结构,减小胎体成型时的变形;在二段成型时用新型高精度的齿轮齿条传递环结构,同步性高,夹持胎冠进行三角度点对称打压,提高胎坯圆度和均匀性.通过该二次法成型机成型的胎胚提高了动平衡性能、均匀性等关键性能指标,推动高精度乘用车轮胎工业的发展.     

面向Ghost光滑粒子动力学模拟的图形处理器快速邻居搜索算法

提出了一种全新的快速邻居搜索方法,该方法可提高基于光滑粒子动力学的流体模拟在图形处理器上的运行效率。此外,这种新的邻居表建立方法可以对两种或者两种以上的粒子进行邻居搜索,使所有粒子能在同一背景网格下拥有独立的粒子属性。在此基础上,引入了Ghost边界粒子以加强光滑粒子动力学方法在边界模拟上的准确性,从而使流体模拟更加真实。实验证明,与传统的基于图形处理器的光滑粒子动力学模拟相比,本文方法效率更高。     

化橘红中主要活性成分对豚鼠气管平滑肌细胞增殖的影响

目的：初步观察化橘红中主要活性成分对豚鼠气管平滑肌细胞增殖的影响。方法通过原代培养豚鼠气管平滑肌细胞,采用 MTT 法观察化橘红中主要活性成分柚皮苷（Naringin）、橘皮内酯（Meranzin）和水合橘皮内酯（Meranzin hydrate）对细胞增殖的影响。结果柚皮苷（0.2~2.0 mg/mL）对豚鼠气管平滑肌细胞增殖有明显的促进作用（P<0.01）,水合橘皮内酯（0.1~1.0 mg/mL）对豚鼠气管平滑肌细胞增殖有明显的抑制作用（P<0.01）,橘皮内酯（0.05~0.5 mg/mL）对豚鼠气管平滑肌细胞的增殖作用不明显（P ＞0.05）。结论柚皮苷与水合橘皮内酯可能是化橘红主要药效活性成分。     

光滑函数芽的通用形变

文章证明了光滑函数芽的无穷小形变的存在性,进而得到通用形变的存在性,并得到了求通用形变的方法。     

实时任务的选择性压缩

实时任务的动态调度仍然是一个值得研究的问题,特别是在一些新的应用背景下.当一个实时系统已经处于满负荷运行,而又有新任务需要插入或老任务需要加速时,就要有老任务降速(受压)以出让带宽.即使如此,什么时刻能开始这一调整是一个关键问题.关于这一调整时刻的计算,现有研究成果都只是基于一个受压任务给出的.基于EDF(Earlie...     

基于k-trajectory算法的电力线巡检机器人的轨迹平滑方法

研究了一种电力线路巡检飞行机器人轨迹平滑的方法.首先,利用坐标变换将机器人在三维空间中的运动映射到二维平面上;其次,在符合飞行机器人动力学规律的基础上,阐述了在k-trajectory算法基础上进行轨迹平滑的方法,分析了三种情况下机器人的飞行轨迹;最后,通过计算机仿真实验证明了该方法的正确性和有效性.     

Velocity and myosin phosphorylation transients in arterial smooth muscle: effects of agonist diffusion

Summary Transients in myoplasmic [Ca²⁺] and in phosphorylation of the 20,000 dalton light chain of myosin have been reported following stimulation of vascular smooth muscle by various agonists. Since these transients are rapid compared with the time required to attain a steady-state stress, agonist diffusion rates may be a significant limitation in activation. The purpose of this study was to estimate the effect of agonist diffusion rates on the time course of activation as assessed by mechanical measurements of stress development and isotonic shortening velocities and by determinations of the time course of myosin phosphorylation. The approach was to measure these parameters in K⁺-stimulated preparations of the swine carotid media of varying thicknesses and to estimate the theoretical contributions imposed by diffusion rates and the presence of a diffusion boundary layer surrounding the tissue. The results show that the time course of parameters which are tissue averages such as stiffness, active stress, and myosin phosphorylation is dominated by agonist diffusion rates. The sequence of events involved in excitation-contraction coupling including agonist actions on the cell membrane, Ca²⁺ release, activation of myosin light chain kinase, and cross-bridge phosphorylation appear to be very rapid events compared with stress development. Estimates of unloaded or lightly loaded shortening velocities which are not simple tissue averages appear to provide an imporoved estimate of activation rates.Supported by a grant from the National Institutes of Health 5-PO1-HL19242. K. E. Kamm was supported by a National Heart, Lung, and Blood Institute Research Service Award HL-05957.