Characterisation of human skin conductance at acupuncture points

Some physicians use the electrical conductance of the skin, particularly at the acupuncture points, for diagnostic purposes. This paper deals with the quantification of the skin conductance at some acupuncture points under well defined conditions using the electrode materials gold, graphite, silver and brass. The observed current response appeared to be best described by two exponentials.     

The use of indentometry to study the effect of agents known to increase skin c-AMP content

Summary Local, externally applied pharmacological agents which are assumed to raise the c-AMP level, decrease the low pressure indentation value of the forehead skin of certain human volunteers.     

平疹膏治疗皲裂性湿疹的疗效观察

皲裂性湿疹是常见慢性皮肤病,病程长且反复发作.采用中国医科大学老年病防治研究中心研制的平疹膏,治疗皲裂性湿疹26例,疗效显著.     

趋肤效应的理论解释

指出了有2种趋肤效应,对变动电流趋于导体表面的现象,给出了理论解释.     

四肢大面积皮肤撕脱伤的治疗

1994年3月至1999年7月共收治四肢大面积皮肤撕脱23例,经反取皮原位植皮等处理,创面一期修复,肢体功能基本满意。     

体外结扎小白鼠精索对睾丸组织学的影响

应用体外结扎精索的方法，使小白鼠睾丸组织缺血、缺氧，并对其结扎后的睾丸进行组织切片观察，结果表明，由此造成的组织细胞缺血、缺氧对小白鼠睾丸的生精机能有明显的影响．主要表现在曲精小管中各级生精细胞退变，数量减少，排列松散、管壁变薄（细胞层次减少），管腔变大。管腔中有变性细胞和多核巨细胞，失去了特定的细胞组合图象，尤以连续结扎３次的睾丸曲精小管的上述变化更为明显。     

A new approach to analysis of human sweating

In human skin transplanted to the back of 3 strains of immuno-deficient mice the functin of the eccrine sweat glands of the human transplant was tested by topical intradermal application of pilocarine, adrenaline and atropine+pilocarpine. Sweat responses were observed in pre-selected fields of observation by means of video macroscope. The iodine strarch reaction served as an indicator for the appearance of seat sport and permitted the evaluation of areas wetted by sweat in the field of observation. Among 9 animals tested, the hybrids between the CB-17-scid mouse and the BALB/cA-nu mouse (BALB/cA-nu,scid) seemed to exhibit the most consistent seweating response to local pharmacological stimulation. According to histological examination, eccrine sweat glands were preserved in human skin trasplanted into the back skin of the BALB/cA-nu,scid mouse strain. the heterologous, human skin graft provides a novel model permitting, independent of the normal sweat gland innervation, the analysis of moecular receptors of sweat gland cells by which the actions of natural transmitters and pharmacological agents are transduced.     

小鼠皮肤局部缺血模型的建立

目的研究建立稳定的昆明小鼠皮肤局部缺血模型。方法取40只雌性昆明小鼠,随机分为A／B两组,每组20只。分别在小鼠背部建立深达皮下筋膜层的u型皮瓣。A组为3．3×1．5cm皮瓣组．B组为2．5×1．5CITI皮瓣组。皮瓣游离缘垫以医用橡胶,缝合切口,造成皮肤缺血模型,术后观察皮瓣发绀水肿及坏死情况,并用散斑全景实时血流成像仪（MoorFLH）检测皮瓣血流。结果肉眼观察,3．3×1．5cm皮瓣组在术后第2天,50％小鼠皮瓣末端坏死,坏死面积达皮瓣面积的（37．20±4．83）％。术后第3天100％小鼠皮瓣末端坏死。并随时间的延长,坏死面积逐渐扩大。2．5×1．5皮瓣组在术后第1天皮瓣出现发绀,水肿,发绀水肿面积占（77．46±4．51）％,术后第3,第5天发绀水肿区面积逐渐减小,至术后第7天,发绀、水肿完全消退。散斑全景实时血流成像仪（MoorFLPI）检测皮肤血流显示,术后第1天皮瓣显著性缺血（P〈0．05）,术后第7天血流基本恢复到术前水平。结论成功建立了昆明小鼠皮肤局部缺血模型,为进一步研究组织微血管再生的机理和策略提供简单而稳定的动物模型。     

Effect of temperature on in vitro proliferative activity of human umbilical vein endothelial cells

Human umbilical vein endothelial cells, skin fibroblasts, and retinal pigment epithelial cells are cultivated in medium supplemented with 15 to 20% serum in our laboratory. The effects of various incubation temperatures on the proliferation of these cells was examined. Our study shows that the mitogenic response of the endothelial cells to a change of temperature differed markedly from that of the fibroblasts and epithelial cells. Cultivation of human umbilical vein endothelial at 37°C required seeding densities as high as 1–2×10⁴ cells/cm², and yet resulted in a low growth rate and premature senescence. However, under the same culture conditions, but at 33°C, the proliferative capacity of these endothelial cells was potentiated. The results were striking; at 33°C the cells grew actively and the life span was extended. The number of cumulative population doublings increased fourfold compared with that for the same cells cultivated at 37°C. The inoculum size could be reduced, since at 33°C the endothelial cells were able to replicate at seeding densities as low as 20 cells/cm². The cells serially subcultured at 33°C retained morphological features and specific immunological markers of endothelial cells.     

襟翼舵水动力性能研究

对襟舵在螺旋桨尾流中的水动力性能及其桨舵干扰进行了研究，舵的水动力及周围流场用面元法计算，螺旋桨性能通过无限叶数的简易螺旋为预估，桨舵干扰作用以迭代方法求得，由于采用限面元法计算襟翼舵的性能，可以更为精确地反映较为复杂的襟翼舵表面形状对水动力性能的影响，文中还对螺旋桨尾流中反应舵的表面压力分布及操纵性能进行了计算和研讨 。