滚齿机分齿传动链传动精度动态测量方法的探讨

本文着重对笔者提出的传动精度动态测试方法的测量原理、特点、可行性定量分析及测试过程中应注意的问题等进行了比较详细而深入地探讨和论证.本测试方法的试验成功,为采用一个光栅式角位移传感器对滚齿机分齿传动链进行切削状态下传动精度动态测量的可行性提供了可靠的依据.     

优化技术用于交错管道双环连接设计

对双环管连接交错管道问题,从几何分析到目标函数的建立,加上各种约束条件,通过计算机运算,非常迅速、有效地求得各项设计参数,从而满足工程的各种需要。     

陕北长6油层组短期基准面旋回与储层非均质性的关系

鄂尔多斯盆地上三叠统延长组长６油层组为一套砂、泥薄互层的组合类型,储层非均质性很强,尤以层间的非均质性最为严重,明显受基准面升、降,和可容纳空间及沉积物供给量的变化影响。通过高分辨率层序地层的短期基准面升、降旋回过程与储集物性关系的分析,建立了６种不同沉积微相的非均质性模式,探讨了向上变深、向上变浅的非对称型和向上变深后复变浅的对称型短期基准面旋回层序对储层非均质性特征的控制作用     

鞍山市青少年活动中心裙楼结构设计及体会

通过一个工程实例,介绍高层建筑框架结构设计中遇到的工程问题的处理方法     

Connecting Conditions and Constraint Equations in Applied Science

IntroductionIn applied science , e . g . machinery , vibrationmechanics , fluid mechanics , and management science , toobtain the opti mal solution of some issues , the objectfunction is usually shown since the abstract mathematicsmodel is set , and all kinds of boundary conditions andconnecting conditions or constraint conditions are putforward . Above these conditions are shown with the shapesof constraint equations . For example ,in machinery opti maldesign, to get the opti mal solution of …     

重组人脑钠素在大肠杆菌中的表达、纯化与鉴定

人脑钠素(hBNP,humanbrainnatriureticpeptide)是心室分泌的由32个氨基酸组成的多肽激素.临床研究表明,hBNP是治疗充血型心衰竭的一种安全有效的多肽药物.化学合成脑钠素全基因,以pET32a为表达载体,构建了硫氧还蛋白-脑钠素(thioredoxin＿hBNP)融合蛋白表达质粒,并转化至大肠杆菌BL21(DE3),经IPTG诱导,融合蛋白的表达量占全菌蛋白量的25%以上.融合蛋白经肠激酶裂解、Zn＿Sepharose亲和层析和C4反相高效液相层析,从每升培养液中可获取4mgrhBNP,纯度达到95%.经质谱测定,rhBNP样品的分子量为3464Da.体外活性测定结果表明,rhBNP样品对兔胸主动脉条具有显著的血管舒张效应,其ED50为(2.24±0 58)×10-6mg/mL.     

重组人胸腺肽α1在大肠杆菌中的表达纯化和鉴定

用PCR将化学合成的人胸腺肽α1基因扩增并克隆到pMD18-T载体中,经过KpnI／SacI酶切、连接将胸腺肽基因插入到表达载体pET32b中硫氧还蛋白下游．将重组质粒转化至表达宿主BL21(DE3)中,经IPTG诱导,Zn—Sepharose亲和层析纯化以及复性处理,从每升菌液中可获得35mg硫氧还蛋白-胸腺肽α1融合蛋白．经凝血因子Xa酶切、Zn—Sepharose亲和层析以及反相高效液相色谱纯化获得3．8mg重组人胸腺肽．小鼠胸腺细胞凋亡实验表明,重组人胸腺肽能显地抑制地赛米松诱导的小鼠胸腺细胞的凋亡,且该抑制作用与重组人胸腺肽的浓度呈剂量依赖关系．     

Involvement of penaeidins in defense reactions of the shrimp Litopenaeus stylirostris to a pathogenic vibrio

The present study reports for the first time the involvement of an antimicrobial peptide in the defense reactions of a shrimp infected by a pathogenic Vibrio, Vibrio penaeicida. New members of the penaeidin family were characterized in the shrimp Litopenaeus stylirostris by RT-PCR and RACE-PCR from hemocyte total RNAs, and by mass spectrometry detection and immunolocalization of mature peptides in shrimp hemocytes. In infected shrimps, bacteria and penaeidin distribution colocalized in the gills and the lymphoid organ that represented the main infected sites. Moreover, the shrimp immune response to infection involved massive hemocyte recruitment to infection sites where released penaeidin may participate in the isolation and elimination of the bacteria, We show that the ability of the shrimps to circumvent shrimp infections is closely related to a recovery phase based on the hematopoietic process.Received 25 November 2003; received after revision 8 January 2004; accepted 21 January 2004     

Hemokinins and endokinins

The mammalian tachykinins are a family of peptides that, until recently, has included substance P (SP), neurokinin A and neurokinin B. Since, the discovery of a third preprotachykinin gene (TAC4), the number of tachykinins has more than doubled to reveal several species-divergent peptides. This group includes hemokinin-1 (HK-1) in mouse and rat, endokinin-1 (EK-1) in rabbit, and EKA, EKB, human HK-1 (hHK-1) and hHK(4–11) in humans. Each exhibits a remarkable selectivity and potency for the tachykinin NK₁ receptor similar to SP. Their peripheral expression has led to the proposal that they are the endogenous peripheral SP-like endocrine/paracrine agonists where SP is not expressed. Moreover, their strong cross-reactivity with a specific SP antibody leads us to question many of the proposed locations and roles of SP in the periphery. Additionally, three orphan tachykinin gene-related peptides are identified on TAC4, in rabbit, EK-2 and in humans, EKC and EKD.Received 25 January 2004; received after revision 18 February 2004; accepted 27 February 2004