重症急性胰腺炎病人外周血单个核细胞线粒体DNA拷贝数的意义

目的探讨重症急性胰腺炎患者外周血单个核细胞线粒体DNA拷贝数与患者病情严重程度、预后的相关性。方法分离研究对象（重症急性胰腺炎患者34名,健康对照组17名）外周血单个核细胞,采用荧光实时定量PCR检测各组PBMC线粒体DNA相对舍量,其与严重程度、预后的相关性。结果重症急性胰腺炎患者线粒体DNA含量较健康对照组明显降低（P〈0．05）。患者单个核细胞线粒体DNA拷贝教与APACHEⅡ评分呈负相关（r=-0．425）其中并发症组mtDNA拷贝数显著低于无并发症组（P〈0．05）。结论重症急性胰腺炎患者外周血单个核细胞中线粒体DNA含量显著下降,与患者APACHEⅡ评分呈负相关,且低水平的线粒体DNA拷贝数提示并发症发生率的升高,因此,线粒体DNA拷贝数可作为潜在评估患者病情危重程度及预后的指标,这一发现值得进一步大型,前瞻性的研究。     

强烈段塞流特征参数试验研究

为了寻求强烈段塞流特征参数的变化规律,在高4 m、内径50 mm的管线中利用双平行电导探针测试了强烈段塞流的持液率信号,并用互相关法对其进行了分析,得到了强烈段塞流的液塞速度、液塞长度随气、液相折算速度及下倾管倾角的变化规律。结果表明,当气相折算速度恒定时,随着液相折算速度的增大,液塞速度、液塞长度均线性增大;当液相折算速度恒定时,随着气相折算速度的增大,液塞速度线性增大,而液塞长度呈双曲型减小;当气、液相折算速度恒定时,随着下倾管倾角的增大,液塞速度、液塞长度都稍有增大。     

重组SARS冠状病毒S蛋白的原核表达研究

目的克隆表达SARS冠状病毒的主要结构蛋白(S蛋白)。方法合成SARS冠状病毒S蛋白特异性基因片断并克隆入pET32a原核表达载体,转化BL21菌,经IPTG诱导高效表达得到重组S蛋白,并通过W estern印迹对重组蛋白质进行鉴定。结果重组蛋白质经镍柱亲和层析得到了部分纯化,免疫动物后得到SARS病毒的多克隆抗体。结论经E lisa检测,表达的重组S蛋白基本具备检测病人血清中抗SARS病毒IgG和IgM的能力,可进一步用于S蛋白功能研究与SARS诊断试剂盒的研制。     

块体纳米材料的研究现状与发展思路

块体纳米材料的制备技术及性能研究 ,是当前纳米材料领域内的一个热点 块体纳米材料具有奇异的结构和特殊性能 ,其在力学、电学、光学和磁学等方面发生了巨大的变化 文中较为详细地介绍了国内外块状纳米材料的制备技术 针对块体纳米材料加工过程中存在热稳定性及致密性等两个主要问题进行了探讨 ,提出了通过加入第二相微粒、强烈塑性加工措施来改善块体纳米材料的热稳定性 ;采用烧结、挤压辅助工艺来提高块体纳米材料的致密性的方案     

北京地区局地强风暴发生的环境条件

采用１９８０～１９８８年北京地区局地强风暴和北京探空资料，研究了本地区局地强风暴发生的环境条件，有利的环境条件是：对流层低层为低压；层结不稳定和中下层位势不稳定；中低层水汽含量高和对流层深厚气层中相对湿度大．局地强风暴生成与中层干燥、高空强风和垂直风速切变无明显关系．     

澜沧—耿马大震前后尾波Q值的研究

本文根据Aki的尾波散射理论,对澜沧-耿马大震前后近10年的时间,在大震区域及外围的7个地震台近2000多张地震记录图的尾波进行测量,得出Q值随时间变化的结果表明:澜沧、耿马两次大震前有1～3年左右的低Q值异常期;在临近大震前1年左右,Q值处于回升阶段;震后Q值明显增大.并结合澜沧-耿马地区区域地质构造图,试图分析Q值在断层错动中随时间变化的特征;澜沧、耿马7.6、7.2级两次大震是分别孕育的双主震并且可能是澜沧7.6级大震的发生触发了耿马7.2级大震的发生。     

The outbreak pattern of the SARS cases in Asia

The severe acute respiratory syndrome (SARS) caused tremendous damage to many Asia countries, especially China. The transmission process and outbreak pattern of SARS is still not well understood. This study aims to find a simple model to describe the outbreak pattern of SARS cases by using SARS case data commonly released by governments. The outbreak pattern of cumulative SARS cases is expected to be a logistic type because the infection will be slowed down due to the increasing control effort by people and/or due to depletion of susceptible individuals. The increase rate of SARS cases is expected to decrease with the cumulative SARS cases, as described by the traditional logistical model, which is widely used in population dynamic studies. The instantaneous rate of increases were significantly and negatively correlated with the cumulative SARS cases in mainland of China (including Beijing, Hebei, Tianjin, Shanxi,the Autonomous Region of Inner Mongolia) and Singapore. The basic reproduction number R0 in Asia ranged from 2.0 to 5.6 (except for Taiwan, China). The R0 of Hebei and Tianjin were much higher than that of Singapore, Hongkong, Beijing, Shanxi, Inner Mongolia, indicating SARS virus might have originated differently or new mutations occurred during transmission. We demonstrated that the outbreaks of SARS in many regions of Asia were wall described by the logistic model, and the control measures implemented by governments are effective. The maximum instantaneous rate of increase, basic reproductive number, and maximum cumulative SARS cases were also calculated by using the logistic model.     

Impacts of climate warming on heating energy consumption and southern boundaries of severe cold and cold regions in China

Impacts of climate warming on heating energy consumption and southern boundaries of severe cold and cold regions of China in the past 20 years are analyzed by using daily and monthly average tem- perature data from 590 weather stations in China and based on regulations of Heating Ventilation and Air Conditioning Design Rules (GB50019-2003) and Thermal Design Rules for Civil Building (GB50175-93) (China National Standard). The contribution of climate warming to coal saving for heating during cold seasons in major cities is calculated according to indices of coal consumption for heating in major cities during cold seasons defined in Energy Conservation Design Standard for New Heating Residential Buildings (JCJ26-95). Comparing with the period before 1980, southern boundaries of se- vere cold and cold regions shift toward north up to 2 degrees in latitude since the mid-1980s. Theo- retically, climate warming could contribute to 5%―10% coal savings for heating since the mid-1980s in major cities, and even more since the mid-1990s.     

板片空间结构高层建筑的研究

对板片空间结构体系用于高层建筑结构情况下结构的力学性能，动力及抗震，风载反应等进行了研究，并与一般钢筋混凝土结构进行了比较，分析结果表明板片空间结构高层建筑具有受力合理，自重轻，抗震性能较好和基础工程的费用较少的优点。