Interaction of aloe-emodin with human serum albumin

The presence of several high affinity binding sites on human serum albumin (HSA) makes it a possible target for many drugs. This study is designed to examine the effect of aloe-emodin on HSA by fluo-rescence, CD spectroscopy and molecular modeling. The results of fluorescence measurements sug-gested that the hydrophobic interaction was the predominant intermolecular force stabilizing the AE-HSA complex, which was in good agreement with the result of molecular modeling study. And the enthalpy change ΔH0 and the entropy change ΔS0 were calculated to be -7.041 kJ·mol-1 and 76.619 J·mol-1·K-1 according to the Van't Hoff equation. The alterations of protein secondary structure in the presence of AE in aqueous solution were quantitatively calculated from CD spectra, and the content of α-helices obviously increased.     

SD大鼠肝癌癌前病变血清微量元素含量变化分析

目的:用DENA为诱变剂,建立不同时期SD大鼠肝癌动物模型,系统的观察肝癌癌前病变发生发展过程中血清Cu,Zn,Fe,Ca,Mg含量的变化.方法:DENA连续灌胃至11周,分别在14 d、28 d、56 d、77 d采血,原子吸收分光光度法测定血清中Cu,Zn,Fe,Ca,Mg 5种元素含量,用SPSS软件进行统计处理.结果显示:在诱癌第14 d,实验组血清Fe含量显著高于对照组(P<0.05),第28 d差异极显著(P<0.01);而血清Zn含量在第28 d实验组显著低于对照组(P<0.05),其它元素实验组与对照组结果无差异.第56 d和第77 d实验组血清Fe、Zn含量都极显著低于对照组(P<0.01).而56 d之后实验组血清Cu、Ca含量都显著高于对照组(P<0.05).整个诱癌过程中实验组和对照组血清Mg含量结果无差异.结论:从14 d到77 d,与对照组比较,实验组血清Cu,Ca含量逐渐升高,而Zn含量逐渐降低,Fe含量先升高后降低,Mg含量在整个过程中没有明显变化.     

碱性介质中茜素黄R与牛血清蛋白相互作用研究

在碱性条件下,采用紫外—可见分光光度法研究了茜素黄R(Alizarin Yellow R)与牛血清白蛋白(BSA)的结合反应。研究表明,AYR—BSA二元配合物的最大吸收波长为386 nm,比AYR紫移约108 nm;建立了碱性条件下以AYR测定蛋白质的光谱分析法,对样品分析表明,回收率为96.4%~106.6%,相对标准偏差为0.28%~1.13%;采用双波长法、平衡透析法和单波长法对AYR与BSA的相互作用的研究结果基本一致,在386 nm处,AYR—BSA配合物的结合比为nAYR:nBSA=5 1,摩尔吸光系数ε=9.12×103L.mol-1.cm-1,相互作用的条件平衡常数K=8.13×105。     

影响青少年身高生长因素分析

目前社会上流行各种各样的增高方法,其中很多是毫无科学道理的,存在严重的误导消费行为。影响青少年身高增长主要因素很多,如睡眠、体育锻炼和营养补钙等,注意这些可以为青少年拥有理想身高提供帮助。中国城市儿童普遍缺乏体育锻炼及睡眠不足应引起高度重视。     

Ecdysteroid receptors located in the central nervous system of an insect

Summary Using thaw-mount autoradiography for steroid hormones, we obtained direct evidence for a nuclear localization of ecdysteroid binding sites in target organs of blowfly (Calliphora vicina) larvae. The binding sites revealed properties of ecdysteroid receptors. Endocrine cells of the ring gland were found to be target tissues of ecydysteroids. This observation provides morphological evidence for a network of complex interendocrine regulation. In the central nervous system receptorcontaining neurons were identified which include many, if not all, neurosecretory cells of the brain. A map of ecdysteroid sensitive cells of the larval brain is presented.     

利用发光细菌测定人体血清杀菌活性的研究

本文报导了以发光细菌作指示生物,以相对发光强度的变化为指标,测定人体血清样品总补体活性(CH_(50))的新方法。当把发光细菌和正常人体血清混和培养时,由于血清的杀菌作用而使细菌体内发光强度减弱,且光强度的减弱程度直接与活菌数的减少以及血清浓度相关联。用这一方法测定了199个人体血清样品,并与常规方法测定结果作比较,表明所有CH_(50)值正常的人血清样品抑制细菌发光能力明显大于CH_(50)值不正常的人血清样品,而且抑制细菌发光50％所得结果与常规CH_(50)试验结果一致。发光细菌测定法是一种简便、快速、灵敏的方法。     

Thyrotropin-releasing hormone does not inhibit lysine vasopressin-induced growth hormone secretion in normal men

Summary In order to establish whether thyrotropin-releasing hormone (TRH) inhibits lysine-vasopressin (LVP)-induced growth hormone (GH) release, six normal men were tested with LVP alone or in combination with TRH. LVP strikingly increased serum GH levels; this response was not altered by TRH. These results indicated that in man TRH is not involved in the control of GH secretion in response to LVP.     

Insulin requirement of human leukemic cell lines

Summary The growth characteristics of human leukemic cell lines in serum supplemented medium and in serum free medium with and without the addition of insulin were investigated. No relation was found between the insulin binding capacity of the cells and their hormone-dependence for growth.This work has been supported by grant CT82.00191.04 of CNR Rome and by Regione Piemonte.     

利用发光细菌测定人体血清杀菌活性的研究

本文报导了以发光细菌作指示生物,以相对发光强度的变化为指标,测定人体血清样品总补体活性(CH_(50))的新方法.当把发光细菌和正常人体血清混和培养时,由于血清的杀菌作用而使细菌体内发光强度减弱,且光强度的减弱程度直接与活菌数的减少以及血清浓度相关联.用这一方法测定了199个人体血清样品,并与常规方法测定结果作比较,表明所有CH_(50)值正常的人血清样品抑制细菌发光能力明显大于CH_(50)值不正常的人血清样品,而且抑制细菌发光50%所得结果与常规CH_(50)试验结果一致.发光细菌测定法是一种简便、快速、灵敏的方法.     

超滤质谱法研究人血清白蛋白与甘草提取物的相互作用

为研究和筛选甘草提取物中的活性成分,采用超滤质谱法(UF-MS)研究了人血清白蛋白和5种甘草提取物(甘草苷、甘草酸、甘草素、甘草呋喃酮和甘草利酮)的相互作用.结果表明:这5种甘草提取物均与人血清白蛋白有不同程度的结合,其中甘草酸的结合能力最强,其次是甘草苷、甘草素、甘草呋喃酮和甘草利酮.说明超滤质谱法可准确识别与受体蛋白质结合的活性小分子配体,实现天然产物活性成分的快速筛选.