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91.
A. Hemminki 《Cellular and molecular life sciences : CMLS》1999,55(5):735-750
Peutz-Jeghers syndrome (PJS) is a classic, but not widely known hereditary trait. Its clinical hallmarks are intestinal hamartomatous
polyposis and melanin pigmentation of the skin and mucous membranes. In addition, PJS predisposes to cancer . The most common
malignancies are small intestinal, colorectal, stomach and pancreatic adenocarcinomas. Other cancer types that probably occur
in excess in PJS families include breast and uterine cervical cancer, as well as testicular and ovarian sex cord tumors. The
relative risk of cancer may be as high as 18 times that of the general population, and the cancer patients' prognosis is reduced.
Recently, the predisposing locus was mapped to 19p13.3 using a novel method. Subsequently, the causative gene was shown to
be LKB1 (a.k.a. STK11), a serine/threonine kinase of unknown function. Although preliminary reports seem to suggest a minor role for LKB1 in sporadic tumorigenesis, further investigations are needed.
Received 12 October 1998; received after revision 30 November 1998; accepted 30 November 1998 相似文献
92.
介绍了CaM激酶的结构和生化特点,以及植物体内CaM激酶的研究进展。从植物细胞信号转导、发育和基因表达等3个方面讨论了CaM激酶激酶在植物体内的生理功能。 相似文献
93.
94.
To explore correlation between the tk gene structure of pseudorabies virus (PRV) and its virulence, to study the effect of the gene mutation on PRV biological properties, and to investigate mechinism of reduced virulence, thymidine kinase (TK)-deficient mutant of pseudorabies virus strain Hubei (PRV HB) was isolated by selection for resistance to 5-bromodeoxyuridine. The tk genes of PRV HB and its TK― mutant were cloned and sequenced. 1587 base pairs of the tk gene and flanking regions of wild-type (wt) virus were sequenced, which included an open reading frame (ORF) of 1098 bp encoding a protein of 366 amino acids. The ORF contained two 137-bp repeated sequences, which were connected by an adenosine. 1458 bp of the tk and flanking regions of TK― mutant were sequenced. Analysis of the tk gene sequence of TK― mutant indicated that one of 137 bp repeated sequence and the connecting adenosine in the tk gene of the wt virus was deleted and a repeated sequence of 8 nucleotides (GCGCGCC) was inserted. All other nucleotides of TK―mutant were identical to that of wt virus. Deletion and insertion of the nucleotide sequence resulted in a frameshift and a premature chain termination, and the resultant TK protein was not active. Analysis of the amino acid sequence revealed that TK protein of PRV HB contained the conserved consensus sequence of herpesviral TKs and an additional conserved-DHR-motif. The results of this work also indicated that TK― mutant was genetically stable. Compared to PRV HB, virulence of TK― mutant was greatly decreased. Mice vaccinated with TK― mutant were completely protected against a lethal challenge with virulent PRV (HB). 相似文献
95.
U. Mrwa K. Güth J. C. Rüegg R. J. Paul S. Boström R. Barsotti D. Hartshorne 《Cellular and molecular life sciences : CMLS》1985,41(8):1002-1006
Summary The contraction induced by a Ca2+-independent myosin light chain kinase (MLCK-) was characterized in terms of isometric force (Fo), immediate elastic recoil (SE), unloaded shortening velocity (Vus), shortening under a constant load and ATPase activity of chemically skinned smooth muscle preparations. These parameters were compared to those measured in a Ca2+-induced contraction to assess the nature of cross bridge interaction in the MLCK-induced contraction. Fo developed in chicken gizzard fibers as well as SE were similar in contractions elicited by either agent. Vus in the contraction induced by MLCK-(0.36 mg/ml) was similar though averaged 39.3±8.9% less than Vus induced by Ca2+ (1.6x10–6M) in the control fibers. Addition of Ca2+ (1.6x10–6M) to a contraction induced by MLCK-resulted in small increases in both Fo and Vus. Shortening under a constant load was similar for both types of contractions. The contraction induced by MLCK-was accompanied by an increased rate of ATP hydrolysis. The MLCK-induced contraction is thus kinetically similar though not identical to a contraction induced by Ca2+. We conclude that with respect to actin-myosin interaction, MLCK- and Ca2+-induced contractions are similar. 相似文献
96.
The SR-protein kinase activity was analyzed and the cytological changes were observed during oocyte maturation in bisexual transparent color crucian carp ( Carassius auratus color variety) . The results revealed that the SR-protein kinase activity was sensitive to the artificially induced spawning hormones, and the change of oscillatory activity was similar to that of the maturation-promoting factor (MPF) kinase that regulates meiotic cell cycle in fish. 相似文献
97.
几种脊椎动物红细胞血型的比较研究 总被引:2,自引:2,他引:0
采用两种方法对脊椎动物各纲的代表动物小白鼠、家兔、家鸽、蟾蜍、鲫鱼的红细胞血型进行了研究 .方法一 :采用人类抗A、抗B标准血清与上述动物的红细胞进行反应 ,结果均未出现凝集现象 ,表明 :这几种动物的红细胞膜上 ,没有与人类抗A、抗B标准血清起反应的A凝集原和B凝集原 .方法二 :采用同种动物的血清与同种动物不同个体的红细胞进行反应 ,结果 :上述几种动物的红细胞与同种内不同个体的血清均不发生凝集反应 . 相似文献
98.
BCR—ABL融合基因表达的肿瘤蛋白是慢性髓性白血病的主要致病因素,应用人BCR—ABL,基因转染的细胞株FD—rv 210与其祖代细胞FDC—P1细胞组成的配对细胞株和不同的培养条件,建立了一个BCR—ABL酪氨酸激酶抑制剂的体外筛选体系,用于检测BCR—ABL酪氨酸激酶抑制剂的选择性,发现两种BCR—ABL,酪氨酸激酶抑制剂AG957和AG490,对BCR—ABL阳性细胞和阴性细胞的增殖抑制作用相似;培养体系中有无祖代细胞增殖所需的细胞因子,对其增殖抑制作用无影响;AG957短时作用于细胞即发生不可逆的增殖抑制现象,与已知的选择性BCR—ABL酪氨酸激酶抑制剂STI571特性明显不同,提示这两种抑制剂作用呈非选择性,所建立的体外培养体系及不同的培养条件的组合,可用于筛选各种选择性的BCR—ABL酪氨酸激酶抑制剂。 相似文献
99.
应用酶活测定的方法分析了肌酸激酶在不同浓度的乳酸、丙酮酸中的活性变化.实验结果说明在失活过程中没有蛋白质的聚沉,失活与乳酸浓度、丙酮酸的浓度呈正相关.通过对比实验结果提示,丙酮酸离子、乳酸离子对肌酸激酶没有明显影响,丙酮酸、乳酸是通过其解离状态的H+改变了机体pH值而影响肌酸激酶活性的,作者就丙酮酸、乳酸对肌酸激酶影响及其意义进行探讨. 相似文献
100.
通过阐述我国动物血清规模化生产的现状,对其分类、应用和国内外的质量控制标准作了简要的介绍. 相似文献