陕西3种蝮蛇血清及酯酶同工酶研究

采用聚丙烯酰胺聚胶电泳研究了陕西境内3种蝮蛇：日本蝮短尾亚种（Agkistrodon blomhof-fii brevicaudus）、秦岭蝮（A.qinlingensis）及中介蝮（A.intermedius）的血清及酯酶同工酶。结果表明,3种蝮蛇的血清及酯酶图谱显示一定程度的共性,又存在明显差异,支持形态学将其划分为3个独立种的观点;秦岭蝮与中蛤蝮之间的亲缘关系较近,与日本蝮间的亲缘关系较远。     

Spectrofluorimetric determination of magnesium (II) with 7-(8-Hydroxy-3, 6-disulfonaphthylazo)-8-hydroxyquinoline-5-sulfonic acid

A new fluorescent reagent, 7-(8-hydroxy-3, 6-disulfonaphthylazo)-8-hydroxyquinoline-5-sulfonic acid (HDNHQ) for the determination of magnesium has been developed. It reacted with magnesium to form a 1∶1 fluorescent complex withλ _ex/λ _em immediately at room temperature in ammonia-ammonium chloride buffer (pH 10.7). A linear relationship was obtained in the magnesium concentration range of 0–160 ng·mL⁻¹ with the detection limit of 0.04 ng·mL⁻¹. The proposed method was simple, rapid and sensitive. It has been successfully applied to the determination of trace magnesium in blood serum with recoveries of 103.75% and 98.16%, respectively. Foundation item: Supported by the Zi-Qiang Foundation of Wuhan University Biography: Zhang Xian (1975-), female, Ph. D candidant, research direction: organic reagent synthesis and analysis.     

Application of FLUPD-DD-PCR to the study of mRNA expression of glioma cells cultured under the condition of serum starvation

Fluorescently labeled universal primer directed differential display polymerase chain reaction technique (FLUPD-DD-PCR), an improved DD-PCR, is reported for the study of mRNA expression of glioma cells cultured with the serum starvation. The fluorescently labeled universal primer (FLUP) facilitates analysis of PCR products on an auto-sequencer. Compared with the traditional DD-PCR, FLUPD-DD-PCR has advantages of separation of target bands and measurement of mRNA expression quantitatively by adopting Cy5-labeled universal primer. And then the silver staining method has been used to recover the bands for use as template in re-pcr. In this study, 4 differential ESTs, of which 3 are novel ESTs, have been obtained. This work indicates some special novel genes, which can be induced to express under serum absent condition, are involved in coping with serum starvation stress on glioma cells growth.     

论日语专业的基础教学

基础教学是外语教学的重要组成部分 .本文阐述日语语音、词汇、句型、语法等教学环节中值得注意的一些问题 .强调对于不同的教学内容应该采取不同教学法 ,在研究国内外教学的基础上 ,努力创造出符合我国国情的外语教学新方法 .     

水解血清白蛋白对紫杉醇的化学修饰

紫杉醇对卵巢癌和乳腺癌有良好的疗效 ,但它不溶于水 ,使临床应用很不方便 .用水解的牛血清白蛋白修饰紫杉醇 ,可提高其水溶性     

MTT法测定人表皮癌细胞的抗药性

在用ＭＴＴ测定细胞存活性率以研究人表皮细胞ＫＢ－３－１及其抗阿霉素细胞株ＫＢ－Ａ－１对化疗药物阿霉素,长春花碱和秋水仙碱的多药抗性时,发现培养基中血清浓度的变化对ＭＴＴ法测定结果有影响,吸光值在血清浓度为ｗ＝０．０２时最高,ｗ＝０．３０时最低,ｗ＝０．０５－０．２０之间波动不大。而不加血清使甲 溶解困难而产生误差。培养基中的血清（常为ｗ＝０．１０）不影响ＭＴＴ法的结果,在完全培养基中用ＭＴＴ法测定     

血清中盐酸洛美沙星的测定及荧光光谱特性

用荧光分光光度法研究了盐酸洛美沙星在血清中的荧光光谱特性,并对其在血清样品中的含量进行了测定．用乙腈沉淀蛋白对血清进行预处理,在硼酸盐缓冲溶液中,用硫酸调节酸度,并使其pH值为3．0,以β环糊精为荧光增敏剂．通过扫描其激发光谱和发射光谱,可知盐酸洛美沙星在血清中有以下荧光特性盐酸洛美沙星血清提取液和盐酸洛美沙星的标准溶液具有相同特征的激发光谱和发射光谱,其激发波长为288nm,发射波长为450nm,也即药物光谱不受血清成分干扰,但血清样品对盐酸洛美沙星的荧光强度造成强烈的荧光猝灭．测定血清中盐酸洛美沙星的线性范围为0．v5～5．0μg·ml,其回归方程为y＝-0．2370+4．809X(R＝0．9968),血清中盐酸洛美沙星的平均回收率为99．5％,方法的相对标准偏差为0．82％(n＝5)．     

东辽河上下游非点源污染特征对比分析

采用径流分割法估算东辽河下游非点源负荷,对比分析东辽河上下游非点源污染特征,为东辽河水环境治理提供科学依据。结果表明,东辽河下游非点源COD占比为61%~83%,非点源氨氮占比为55%~69%,均高于上游地区;这与同时期农业种植面积紧密相关。东辽河上下游非点源污染负荷年内分布不均,上下游非点源COD负荷与降水量、流量一致,峰值均为7月;与COD不同的是,上游非点源氨氮与降水量、流量一致,峰值7月份;但是下游因玉米种植面积大及8月份追肥的影响,下游非点源氨氮比降水量、流量滞后1月,氨氮峰值为8月份。     

国内外高校教育教学质量评价之比较及启示

通过对国内外高校教育教学质量评价的比较,找出其异同及成因,指出国内目前教育教学质量评价中存在的不足,分析了教育教学质量评价的发展趋势。同时,借鉴国外教育教学质量评价模式,对国内构建多主体、多客体、多类型的评价体系等进行了相关思考。     

头孢他啶与牛血清白蛋白结合的光谱法研究

用荧光光谱法、紫外-可见吸收光谱法和傅立叶红外光谱法研究了牛血清白蛋白与头孢他啶间的相互作用机制.结果表明:在人体生理条件下头孢他啶可使牛血清白蛋白的荧光强度显著降低,二者在不同温度下的结合常数分别为KLB=1.399×106(24℃),KLB=7.501×105(37℃).头孢他啶与牛血清白蛋白主要以疏水作用力和氢键相结合而非单一作用力,为探讨药物头孢他啶在生物体内与蛋白质的作用机理和生物学效应提供了理论依据.