Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines

Adipose tissue is an endocrine organ capable of secreting a number of adipokines with a role in the regulation of adipose tissue and whole-body metabolism. We used two-dimensional gel electrophoresis combined with mass spectrometry to profile the secreted proteins from (pre)adipocytes. The culture medium of 3T3-L1 cells during adipocyte differentiation was screened, and 41 proteins that responded to blocking of secretion by 20°C treatment and/or brefeldin A treatment were identified. Prohibitin, stress-70 protein, and adhesion-regulating molecule 1 are reported for the first time as secreted proteins. In addition, procollagen C-proteinase enhancer protein, galectin-1, cyclophilin A and C, and SF20/IL-25 are newly identified as adipocyte secreted factors. Secretion profiles indicated a dynamic environment including an actively remodeling extracellular matrix and several factors involved in growth regulation.Received 15 June 2004; received after revision 26 July 2004; accepted 2 August 2004     

Porcine factor V: cDNA cloning, gene mapping, three-dimensional protein modeling of membrane binding sites and comparative anatomy of domains

Factor V is a plasma protein essential for blood coagulation. This protein is involved in activated protein C resistance, the most common inherited thrombotic disorder known. We utilized the polymerase chain reaction to clone the porcine factor V gene by generating overlapping clones amplified with primers chosen by comparison with known nucleotide sequences. The porcine factor V cDNA contig encodes a predicted 2258-amino acid protein, making it the largest in comparison to the bovine, human, and murine proteins. Porcine factor V has the highest level of homology with bovine factor V, but also has high levels of conservation of important residues with all the species. Radiation hybrid mapping assigned the porcine factor V gene to chromosome 4. Three-dimensional models of factor V were generated and used to analyze membrane-binding sites in terms of conserved, and therefore likely important residues. Received 3 October 2000; revised 23 November 2000; accepted 6 December 2000     

心灵的传承——试论《简爱》对《蝴蝶梦》的影响

本文以新的视角,对欧洲两部著名文学作品《简爱》与《蝴蝶梦》进行了详尽的比较分析,并从人物刻画、结构设置和艺术处理等多方面入手,剖析了作品成功的审美因素,进而透视出了文学遗产复杂微妙的继承关系。     

Dealing with a Differentiated Whole: The Philosophy of the WSR Approach

This paper seeks to explore a philosophical ground for the formulation and practice of an Oriental systems methodology, the wuli–shili–renli approach (WSR). Drawing insights from ancient Chinese thought, WSR contends that, in sociotechnical systems design/management, we should investigate and follow wuli, shili, and renli, which constitute a differentiable, dynamic whole. Wuli, shili, and renli condition and determine the fate of our projects, meaning different methods are needed to deal with each differentiable aspect. It also points out that, to deal with wuli, shili, and renli properly, we need to engage in encounters with the "dark side" of Confucianism and with the contemporary tendency of scientism.     

农业技术进步对中西方历史灾荒的影响

中西方灾荒历史灾荒均产生了严重的社会后果，在灾荒威胁下，农业技术水平、农业生产结构、农业发展状况等因素方面存在的差异直接影响到自然灾害的成灾后果。农业技术的进步在预防和控制灾荒的活动中发挥了重大作用，中国传统农业科技在一定程度上控制了灾情的蔓延，减轻了灾害的破坏和威胁；也是由于农业技术的落后，欧洲农业遭受灾荒后往往一蹶不振。中国农业发展的整体一致性既有利于技术的普及推广和传播，但也为农业灾荒的发生创造了条件。     

基于比较优势的吉林省种植业结构调整

对吉林省主要农作物的效率比较优势指数、规模比较优势指数和综合比较优势指数进行了计算和分析,结果表明吉林省在玉米、水稻等大宗农作物生产效率上同全国相比没有明显优势;甜菜、烟叶、油料、麻类等经济作物同全国相比处于劣势;玉米、高粱、大豆、甜菜4种作物同全国相比有规模优势,且玉米、高粱优势较大,其它作物处于劣势;吉林省玉米、高粱、大豆同全国相比具有综合比较优势,其它作物处于劣势。将吉林省各地区主要农作物的综合比较优势指数划分为4级,与之相对应调整措施,从而确定了各地区的优势组合,为各地区提出了农作物种植结构调整方向。表10,参16。     

3-甲基-6-氨基-5-氰基-4-（4-对甲氧基）苯基-1-苯基-1，4-二氢吡喃并[2，3-c]吡唑与牛血清白蛋白相互作用的研究

采用荧光光谱法研究了人体生理pH值条件下,3-甲基-6-氨基-5-氰基-4-(4-对甲氧基)苯基-1-苯基-1,4-二氢吡喃并[2,3-c]吡唑(Ⅰ)与牛血清白蛋白(BSA)分子间的相互结合反应;获得了不同温度下Ⅰ与BSA作用的结合常数K和结合位点数n;并根据Forster非辐射能量转移机理,计算出其与BSA相互作用时给体-受体间距离r为5.01 nm及能量转移效率E为0.280.证实了Ⅰ与BSA的相互结合作用为单一的静态猝灭过程,确定了其与BSA分子间有较强的结合作用,且结合力以疏水作用力为主.     

Interaction of aloe-emodin with human serum albumin

The presence of several high affinity binding sites on human serum albumin (HSA) makes it a possible target for many drugs. This study is designed to examine the effect of aloe-emodin on HSA by fluo-rescence, CD spectroscopy and molecular modeling. The results of fluorescence measurements sug-gested that the hydrophobic interaction was the predominant intermolecular force stabilizing the AE-HSA complex, which was in good agreement with the result of molecular modeling study. And the enthalpy change ΔH0 and the entropy change ΔS0 were calculated to be -7.041 kJ·mol-1 and 76.619 J·mol-1·K-1 according to the Van't Hoff equation. The alterations of protein secondary structure in the presence of AE in aqueous solution were quantitatively calculated from CD spectra, and the content of α-helices obviously increased.     

SD大鼠肝癌癌前病变血清微量元素含量变化分析

目的:用DENA为诱变剂,建立不同时期SD大鼠肝癌动物模型,系统的观察肝癌癌前病变发生发展过程中血清Cu,Zn,Fe,Ca,Mg含量的变化.方法:DENA连续灌胃至11周,分别在14 d、28 d、56 d、77 d采血,原子吸收分光光度法测定血清中Cu,Zn,Fe,Ca,Mg 5种元素含量,用SPSS软件进行统计处理.结果显示:在诱癌第14 d,实验组血清Fe含量显著高于对照组(P<0.05),第28 d差异极显著(P<0.01);而血清Zn含量在第28 d实验组显著低于对照组(P<0.05),其它元素实验组与对照组结果无差异.第56 d和第77 d实验组血清Fe、Zn含量都极显著低于对照组(P<0.01).而56 d之后实验组血清Cu、Ca含量都显著高于对照组(P<0.05).整个诱癌过程中实验组和对照组血清Mg含量结果无差异.结论:从14 d到77 d,与对照组比较,实验组血清Cu,Ca含量逐渐升高,而Zn含量逐渐降低,Fe含量先升高后降低,Mg含量在整个过程中没有明显变化.     

甲基红与牛血清白蛋白结合的光谱研究

牛血清白蛋白在不同pH的溶液中存在N(Native,pH～7.0),B(Basic,pH～9.0),E(Expanded,pH3.5以下)等几种结构形态.用光谱技术研究了染料甲基红(Methyl red,MR)和BSA的结合机理,研究了MR和BSA在不同pH的三酸缓冲溶液中的结构和构象变化,荧光猝灭方法测得两者在pH3.0,4.9,7.0,9.0溶液中结合常数分别为7.544×105 L/mol,7.594×105 L/mol,4.728×105 L/mol,4.880×105 L/mol;结合位点数分别为17.89,0.978 3,3.079,8.865.同步荧光显示MR加入对Tyr残基微环境没有影响,但对Trp残基产生微扰,使其最大发射波长发生蓝移.图5,表1,参20.