Serial nuclear transfer improves the development of interspecies reconstructed giant panda (Aluropoda melanoleuca) embryos

Interspecies somatic nuclear transfer (NT) may provide a new approach for preservation of the endangered rare species. Previous interspecies cloning studies have shown that a nucleus from a quiescent somatic cell supports early development of reconstructed embryos in the ooplasm from another species. In this study, we transferred nonquiescent somatic cells from a giant panda into the perivitelline space of the enucleated rabbit oocytes. After electrofusion (at the rate of 71.6%) and electrical activation, 4.2% of the panda-rabbit reconstructed embryos developed to blastocyst in vitro. For improving the development rate of reconstructed embryos, we used serial NT in this study, i.e. blastomeres from reconstructed morulae were transferred into the perivitelline space of the enucleated rabbit oocytes. The fusion rates in the groups of serial I, serial II and serial III were 79.5%, 84.1% and 78.0%, respectively, having no difference with that of somatic group. And the blastocyst rates in serial NT groups were 19.4%, 13.5% and 10.3%, respectively, which are significantly higher than that in somatic NT group. These results indicate that the nuclei from nonquiescent somatic cells can support early development of reconstructed embryos and serial NT can improve the development rate of interspecies reconstructed embryos. These authors contributed equally to this work.     

VB.Net 2003中串行端口通信的实现

针对MSComm控件的不足,探讨在VB.Net2003中如何利用API函数来定义串行端口类,并通过一个应用实例介绍如何利用该类的声明来实现PC与可编程控制器之间的串行通信.这对开发高质量的串行通信有一定的应用和借鉴价值.     

影响密立根油滴实验的因素分析

讨论了近代物理实验——密里根油滴实验,分析了油滴实验中影响实验成败的主要因素,从视场中有无油滴、如何控制油滴、影响油滴的带电因素及如何选择油滴四个方面分析了实验中可能出现的问题并对此提出了相应的对策,为今后的实验提供了教学依据。     

An ERP study for the degrees of the difficulty in visual search

The present study mainly probed the relationships between ERP components and the degrees of difficulty in visual search. Three experimental and one controlled conditions were randomly selected. Results showed that the P1, N1, and P2 amplitudes and latencies had no significant differences among the three experimental and one controlled conditions, and the ERP waveforms of low perceptual load of visual search were different from that of high perceptual load. These results might indicate that the latencies and amplitudes of initial P1, N1 and P2 in high and low perceptual load conditions were almost the same. The ERP waveforms of parallel search were very different from that of serial search, and the ERP waveforms of serial search were very similar. The P3 amplitudes increased as the degrees of the difficulty in visual search decreased.     

PIC单片机在产品开发中程序设计的探讨

对PIC单片机在产品开发中的程序设计进行了分析,并详细讨论了PIC单片机程序计数器(PC)和串口中断数据接收处理程序的设计,对后者给出了部分程序和流程图.     

小波阈值算法的FPGA实现

针对传统的阈值算法排序量多、消耗资源大和速度慢等缺点,提出了一种基于FPGA的快速中值选取器的设计方法.介绍了3点排序器和一种基于3×3点的中值选取器及其MATLAB仿真,针对基于3×3点的全流水并行243点中值选取器的结构利用率低,依据FPGA的硬件特点,设计了一种串并行交替的3n点中值选取器的硬件架构,阐述了快速中值选取器的硬件构架设计,对整个系统进行了仿真,并对仿真结果进行了分析说明.     

夹具CAD中元件设计方法的研究与实践

介绍了一种新的夹具元件设计方法。该方法通过定义基准图和关键参数,经比例变换获得新视图,不需编制参数化绘图程序,又能部分反映元件的实际尺寸,是对夹具元件设计方法的有益补充。     

远地测控管理与实现

中央控制分布式远地测控系统的应用十分广泛 ,其主要问题是主机与分机间的通信。本文对主机的管理策略和通信的实现方法做了简要讨论     

基于89C51智能型太阳热水器的控制系统

介绍了一种基于89C51的智能型太阳热水器控制系统，该系统功能及实用性较强，自动化程度高，并具有初级智能化程度，在水位采集，ADC等设计上采取一些新颖方法，成本低廉，推广价值高。     

PC机与多片DSP的远距离通信

在规模较大，计算、管理较复杂的系统中，采用PC机作上位机，多片DSP作为下位机的建模与实现。