Analysis of cDNA sequence, protein structure and expression of parotid secretory protein in pig

Parotid secretory protein (PSP) secreted abundantly in saliva, whose function is related with the anti-bacterial effect. The PSP cDNA has been isolated from pig parotid glands by 3′ and 5′ rapid amplification of cDNA end (RACE), based on the conserved signal peptide region among the known mammalian PSP. The result of homologous comparison shows that pig PSP and human PSP shares the high identity at the level of the primary, secondary and tertiary protein structure. A search for functionally significant protein motifs revealed a unique amino acid sequence pattern consisting of the residues Leu-X(6)-Leu-X(6)-Leu-X(7)-Leu-X(6)-Leu-X(6)-Leu near the amino-terminal portion of the protein, which is important to its function. RT-PCR, Dot blot and Northern blot analysis demonstrated that PSP was strongly expressed in parotid glands, but not in other tissues.     

Identification and mapping of quantitative trait loci controlling cold-tolerance of Chinese common wild rice (O. rufipogon Griff.) at booting to flowering stages

Cold injury is an important limitation of rice production. Therefore, screening for cold-tolerant genetic resources and the development of highly cold-tolerant cultivars is crucial for higher yield potential and stable yield of rice. There have been several reports on the genetic analysis and QTL mapping of the cold-tolerance at the booting stage. Toriyama et al.[1,2] and Futsuhara et al.[3,4] reported that 4 or more genes were involved in the cold-tolerance. Several QTLs at the booting sta…     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

大豆蛋白纤维纱线的漂白处理

探讨和比较了氧化法漂白和氧化·还原法漂白两种漂白工艺.在氧化法漂白工艺中,分析了温度、H2O2浓度和时间三种重要参数对漂白效果的影响规律,得出了白度能满足染色要求的两种漂白工艺.     

新疆北鲵肌肉蛋白质、氨基酸、无机元素及食物组成分析

本报道了新疆北鲵肌肉蛋白质含量为19．08％，氨基酸17种，无机元素有Ca等6种，成幼体食物组成中以石蚕及腺状介虫为主．     

酶法水解棉籽蛋白的制备与应用

以脱酚后的棉籽粉为原料 ,研究了酶解法制备可溶性棉籽水解蛋白的生产工艺。对其影响因素和产品的实际应用作了探讨。结果表明 ,利用中性蛋白酶AS1.398水解棉籽粉 ,产品得率可达 28.8%,蛋白质量分数为63.28%。棉籽水解蛋白可作为菌体发酵氮源。     

钼酸铵浸种对不同氮源下鲜食油菜产量、品质及对氮磷钾吸收的影响

采用盆栽试验,研究了不同氮源条件下,钼酸铵浸种对油菜产量、品质及氮磷钾(NPK)吸收的影响。结果表明:NH4NO3+Mo对提高鲜食油菜的鲜重和干重具有显著作用;NH4Cl+Mo对油菜Vc含量和叶绿素含量具有显著的提高作用,NH4NO3和(NH4)2SO4与钼配施效果不及NH4Cl。同一时期内的油菜植株Vc含量与叶绿素含量呈正相关;在NPK的吸收方面,不同种类氮肥的阴、阳离子与钼互作效应不同,硝酸铵与钼配施时,钼与NPK均表现协同作用,而NH4Cl和(NH4)2SO4与钼配施时,钼与氮、磷表现协同作用,而钼与钾表现拮抗作用。     

新基因ZNF325在人类早期胚胎中的表达

锌指蛋白基因家族是人类最大的基因家族之一，目前已知的很多转录调控因子都是锌指蛋白成员^[1]。初步克隆了一个与RBAK基因有着高度同源性的人类C2H2型锌指蛋白新基因，经国际基因命名委员会批准命名为ZNF325，此基因位于染色体7p22，长2697个碱基，含5个外显子，在基因组DNA上长15kb。它编码的蛋白质全长462个氨基酸，含15个C2H2型的锌指。Northern Bolt分析表明该基因在人类早期胚胎的各个组织中普遍表达，在肺和脑中的表达水平最强，但在肝中的表达随着胚胎的发育而逐渐减少。它的结构和表达特征预示着它编码的是一个具DNA结合功能的转录调控因子。     

核衣壳蛋白VP39结构分析与杆状病毒的进化研究

在测定了BmNPV—Ch(中国株)和HaMNPV vp39基因序列的基础上，推导出相应的氨基酸顺序，并与AcMNPV、OpMNPV、LdMNPV、BmNPV—Ja(日本株)的VP39蛋白的氨基酸顺序进行了比较。分析结果表明：在已知的各种杆状病毒中，VP39蛋白是一个保守性较强的蛋白质，Bm—NPV—Ch VP39蛋白与AcMNPV、OpMNPV、LdMNPV、BmNPV—Ja和HaMNPV VP39蛋白的氨基酸同源性分别是93．7％、58．2％、39．9％、97．1％、92．8％。HaMNPVVP39蛋白与AcMNPV、OpMNPV、LdMNPV、BmNPV—Ja和BmNPV—Ch VP39蛋白的氨基酸同源性分别为97．3％、59．0%、40．2%、93．1％、92．8％。通过氨基酸亲水性分析比较，探讨了VP39蛋白结构与杆状病毒进化间的关系。