批量数据入库在ASP中筛选重复的处理方法——以湘西民族职业技术学院新生信息录入库为例

在建立数据库时,重复数据的判断对于数据库管理至关重要,没有准确关键词作为对比,重复数据的判断将变得非常困难.传统的哈稀技术、固定分块技术、滑动块技术、可变分块技术和数据指纹等对重复数据进行查找与删除时,占用了大量系统处理时间,且准确性较低.为提高数据处理效率,提出了ASP批量数据在入库时的查重筛选方法,实践验证了该方法的鲁棒性与可靠性,极大地减轻了操作员对数据库管理的繁重工作.     

向日葵Pst Ⅰ和Mse Ⅰ内切酶组合AFLP标记体系的建立及引物筛选

 为建立并优化适合向日葵的Pst Ⅰ 和Mse Ⅰ 组合的AFLP 技术体系, 为下一步构建遗传连锁图谱及开展分子标记辅助育种奠定基础, 对向日葵基因组DNA 提取方法、酶切连接体系和选择性扩增程序的影响因素进行优化, 并对适合向日葵AFLP 分析的引物组合进行筛选。结果表明, 模板DNA 的提取采用改进CTAB 法, 酶切连接采用一步法反应14 h, 在选择性扩增体系20 μL中, Mg²⁺和dNTP 浓度分别为2 mmol/L 和0.3 mmol/L, 选扩引物的浓度为0.6 mmol/L, 预扩增产物最适稀释倍数为30 倍。同时筛选出了稳定且多态性丰富的48 对适合向日葵AFLP 分析的引物组合。     

基于眼睛状态识别的疲劳驾驶检测

由于光照变化、头部姿态等因素的影响,现有的疲劳驾驶检测精度仍有待提高。针对该问题,提出一种基于迁移学习的眼睛状态识别网络(Gabor and LBP-convolutional neural networks,GL-CNN),该网络是由Gabor特征和LBP特征通过迁移学习加入卷积神经网络(convolutional neural networks, CNN)调制组成的。首先用多任务级联卷积神经网络(multi-task CNN,MTCNN)检测驾驶员的人脸和双眼,然后经过眼睛筛选机制获取待检测的单只眼睛,通过GL-CNN识别眼睛的睁闭状态,最后根据PERCLOSE准则判断驾驶员的疲劳状态。实验结果表明,该算法具有较高的准确率,可以检测多种姿态眼睛的状态,同时满足实时性的要求。     

纤维素降解菌的筛选与诱变育种

采用羧甲基纤维素(CMC)平板初筛和50℃培养,筛选到一株纤维素酶活力较高的耐高温霉菌MY菌株,其CMC培养基最适pH为6.0,培养温度为40℃,最佳产酶时间为5d.以MY菌株为出发菌株,分别采用紫外线和硫酸二乙酯诱变,以透明圈直径与菌落直径的比值(HC值)提高30%以上或菌落形态发生明显变异为筛选指标,共筛选到97株突变株;通过测定粗酶液CMC酶活力,从中筛选出29株CMC酶活力提高30%以上的菌株;结合突变菌株的传代稳定性实验,最后筛选得到1株性能优良的霉菌MY004突变株,其CMC酶活力比原始菌株MY提高了80.6%.     

产品本体筛检方法及其在变型没计中的应用

提出了一种应用于产品变型设计中的本体筛检方法,该方法以完整的产品资源本体视图为基础,将产品的功能需求、结构特征等转化为需求约束,设计人员根据需求约束对产品资源本体进行筛检。针对不同的筛检层次和筛检对象,给出了模式匹配、实例匹配与人工决策相结合、实例推理自动匹配等筛检规则。这种人机结合的半自动筛检方法提高了产品变型设计方案的准确性,增加了设计过程的柔性和效率。开发了一套针对建筑门窗产品的变型设计支持系统,证明所述方法的可行性和有效性。     

三株甘蔗糖蜜酒精发酵高产酵母菌株的筛选

从甘蔗糖厂的废弃物中筛选到3株甘蔗糖蜜酒精发酵高产菌株MF1001、MF1002和MF1003,经rDNAITS序列同源比对鉴定为酿酒酵母(Saccharomyces cerevisiae)的不同菌株。3个菌株均可以完全利用葡萄糖和蔗糖,部分利用棉子糖和半乳糖,均不能利用乳糖和木糖。MF1001发酵甘蔗糖蜜的残糖含量明显低于目前使用的生产菌株,略低于标准测定菌株CICC31149和CICC31279,MF1002和MF1003的残糖含量略低于目前使用的生产菌株。与生产菌株相比,3个菌株在甘蔗糖蜜的生长速率略低,但是维持高菌数的时间较长。按目前甘蔗糖蜜酒精生产的发酵工艺,3个菌株30℃发酵72h的醪液酒精含量分别为14.26%(V/V)、14.48%(V/V)和13.50%(V/V),比目前生产使用的菌株高19.5%~28.6%;37℃发酵40h的醪液酒精含量分别为12.03%(V/V)、12.06%(V/V)和12.14%(V/V),比目前生产使用的菌株高10.0%~11.7%。这3个菌株具有提高甘蔗糖蜜发酵醪液酒精含量的潜在工业价值。     

一株产表面活性剂的菌株的筛选及现场试验研究

从胜利油田油水样中分离到一株高温条件下能够利用烃类产生表面活性剂的菌株A2,经鉴定属于Petrobacter.该菌株对两种原油的降解、降黏和降凝结果表明:对孤岛油的降解率较高,为39.81%,对甘17油的降解率达22.60%;对孤岛油的降黏率可达37.95%,对甘17油的降黏率为25.00%;A2可将孤岛油和甘17油的凝固点降低2.5℃.通过菌株作用岩石表面,表明该菌株对玻璃试片作用后其接触角降低最为明显,高达72%,接近于对照菌样的5倍;石英和灰岩的接触角也分别降低了68%和24%.表明A2具有一定的改变岩石表面性质的能力.增油效果评价实验结果表明:A2菌在中一驱Ng3油藏条件下,微生物驱提高采收率9.3%.现场实施后取得了降水增油效果,试验区含水下降2.1%,累积增油0.36×104t,提高采收率0.21%.     

Functional expression of mammalian opioid receptors in insect cells and high-throughput screening platforms for receptor ligand mimetics

Lepidopteran cell lines have been engineered to constitutively express high levels of mouse opioid receptors either alone or in combination with human G16 protein. Biochemical and pharmacological studies demonstrate that these lines contain all the mediator G proteins and downstream effectors required for opioid receptor function, including phospholipase C, and that expression of exogenous G16 does not contribute significantly to increased receptor responses upon activation. The activation of the phospholipase C pathway in the transformed cells upon stimulation with known receptor ligands results in easily and quantitatively measurable increases in free intracellular calcium, which can be monitored by automated fluorescent methods, while the addition of specific antagonists blocks the agonist-induced responses. Therefore, the transformed lepidopteran cell lines can be used as sensitive high-throughput screening platforms for fast detection of opioid receptor ligand mimetics (agonists and antagonists) in collections of natural products and synthetic compounds.Received 3 December 2004; received after revision 3 February 2005; accepted 10 February 2005L. Swevers and E. Morou contributed equally to this work.     

Physicochemical profiling in drug research: a brief survey of the state-of-the-art of experimental techniques

This review begins with a general presentation of the new paradigm of drug discovery, with its emphasis on the rapid identification and elimination of compounds with unsuitable physicochemical and pharmacokinetic properties. The focus of the paper is on the various experimental methods used to determine such key physicochemical properties as ionization, lipophilicity and distribution in isotropic and anisotropic systems, solubility, and permeability across artificial membranes. Both traditional and high-throughput methods are presented and their limits highlighted. The text concludes with the trade-off between quantity/speed in high-throughput screening techniques versus greater data quality in the more labor-intensive methods. Received 23 April 2002; received after revision 25 June 2002; accepted 11 July 2002 RID="*" ID="*"Corresponding author.