Tobacco curly shoot virus iso-lated in Yunnan is a distinct species of Begomovirus

Virus isolate Y1 was obtained from tobacco showing curly shoot symptoms in Baoshan, Yunnan Province. Whitefly transmission test and virion morphology observation showed that it is a begomovirus. In reactions with 14 monoclonal antibodies raised against begomoviruses, Y1 was readily differentiated from begomoviruses reported in China, Pakistan and India. The complete nucleotide sequence of DNA-A was determined, it contains 2746 nucleotides, with two ORFs in virion-sense DNA and four ORFs in complementary-sense DNA. Comparisons with total DNA-A, intergenic region and deduced amino acid sequences of individual ORFs showed that Y1 is a distinct Begomovirus species, for which the name Tobacco curly shoot virus (TCSV) is proposed. The total DNA-A of TCSV is most closely related to that of Tomato leaf curl virus from India (85% sequence identity). In contrast, the deduced coat protein of TCSV is most like that of Cotton leaf curl virus 72b isolate from Pakistan (98% amino acid sequence identity).     

Characterization and relative abundance of maxi-chloride channels in Epstein-Barr virus (EBV) producer: B95-8 cells

Several Epstein-Barr virus (EBV)-transformed cell lines were used to investigate the pathogenesis of lymphoproliferative diseases and nasopharyngeal carcinoma. The studies focus on the events occurring inside the membrane. On only one occasion, the cell membrane of EBV-transformed B lymphocytes from a cystic fibrosis patient was found to express defective Cl channels (CFTR; Cystic Fibrosis Transmembrane conductance Regulator), as in the airway epithelial cell. No other type of channel in EBV-transformed cells has so far been investigated. In this study, the cell membrane of the B95-8 cell was examined by the patch-clamp technique and compared to the non-EBV-infected BJAB cell. The high conductance (300 pS) maxi-chloride (Cl) channel activity was the most frequently observed event in inside-out configurations. Under similar experimental conditions, we have found a significantly higher probability of detecting maxi-Cl channel activity on the cell membrane of B95-8 cells (69%) than on BJAB cells (27%), or as previously reported on resting murine B lymphocytes (38%) or intact human T lymphocytes (37%). The relative abundance of the maxi-Cl channel on B95-8 cells may be linked to EBV infection and/or secretory ability.     

光照对心叶烟叶绿素含量及抑制烟草花叶病毒侵染的影响

在不同光照强度处理下,测定了心叶烟叶片中叶绿素含量及接种烟草花叶病毒后生成的枯斑数,结果表明:随着光照强度的降低,叶绿素含量逐渐减少,而半叶平均枯斑数呈增加的趋势,两者之间具有负相关性,且相关系数r=-0.95.     

关于网络安全问题的研究

介绍了网络安全的概念,分析了Internet中存在的主要安全隐患,重点讨论了网络防火墙技术、加密技术、PKI技术、虚拟专用网技术和安全隔离技术的主要特征及其应用。     

Generation of VP5 deficient mutant of infectious bursal disease virus strain HZ2

Infectious bursal disease virus (IBDV) is one of the most significant viral pathogens in chickens[1]. It causes high mortality in young chickens and establishes an immunosuppression state by destroying the precursorsInfectious bursal disease virus (IBDV) …     

利用柑橘茎段诱导不定芽改进微芽嫁接技术的研究

试验以成年态纽荷尔脐橙茎段为外植体，诱导产生不定芽改进微芽嫁接成活率与脱毒率．结果表明：半木质化茎段作为培养外植体污染率最低，不定芽诱导的最佳培养基为MT＋＋6-BA1．0mg／L＋NAA0．2mg／L＋GA30．2mg／L．将诱导的嫩芽进行微芽嫁接，成活率超过80．8％，对照成活率为61．3％，聚合酶链式反应（PCR）检测脱毒率达100％．     

基于典型蠕虫病毒hezhi的计算机病毒分析

讨论了传统计算机病毒和网络蠕虫病毒的特点和危害,并用OD(动态)和IDA(静态)的方法分析和研究了2004年出现的典型蠕虫病毒(hezhi).揭示了传统计算机病毒尤其是网络蠕虫病毒的原理和机制,同时提出有效的防治措施.     

基于多Agent系统的计算机病毒免疫检测模型

目的 研究在局域网内检测、防范病毒的智能型病毒检测模型。方法 应用多Agent技术与生物免疫系统原理相结合的方法设计计算机病毒检测模型。结果 模型继承了生物免疫系统原理，并结合了多Agent系统的推理性和协作性等特点，具有较强的快速反应能力及自我演化功能等特点。结论 生物免疫原理与多Agent技术相结合可快速有效地检测局域网内的病毒，并能提高计算机对病毒的免疫能力。     

仙台病毒的结构和感染动物的诊断

仙台病毒是引起啮齿类动物呼吸道疾病的病原，它是具有包膜、线性、非片段的负链RNA病毒，可通过血清学方法和分子生物学技术进行检测。     

苜蓿花叶病毒复制酶基因全长cDNA植物表达载体的构建

将苜蓿花叶病毒中国分离株（Alfalfa mosaic virus Chinese isolate，AlMV-Ch）的复制酶P2亚基（90KD蛋白）基因的全长cDNA构建到植物表达载体pROK Ⅱ中，得到重组植物表达载体pAlMV-FL．