基于电子商务的逆向物流优化模型及其应用策略

由于通过电子商务购买商品不能在购买前进行商品实体的检验，所以增加了退货的可能性，因此电子商务中的退货逆向物流问题是困扰其发展的一个重要因素。本文通过构建利润最大化模型，得到了在特定市场反应参数下的最优价格以及最优退货策略，并且通过分析市场反映参数得出了一些具有实际操作意义的应用策略。     

龟鳖类血细胞研究的进展

文章概述了龟鳖类血细胞——红细胞、有粒细胞(包括嗜酸性粒细胞、嗜喊性粒细胞、嗜天青粒细胞和嗜中性粒细胞)、淋巴细胞、单核细胞、血栓细胞的特性,显微和超微结构特点,血细胞发生及功能等方面的研究进展.     

电解法制备次磷酸

研究了以惰性电极为阴阳极，次磷酸钠为原料，在直流电场的作用下，电解生成次磷酸的最佳反应条件和有关工艺。该方法简便高效，投资小，无三废，属于清洁生产。     

DXF文件接口程序的设计及其在反求设计中的应用

DXF文件是一种简单、方便、易读的图形数据转换格式。在反求设计中需将经过精度分析后的轮廓曲线、曲面转入通用CAD /CAM中以充分利用现有资源、提高设计效率。文章根据DXF格式要求,编制了一用于反求设计的DXF格式转换程序应用于实践。     

铝电解槽焙烧用矩形高速燃烧器的研制

铝电解槽的焙烧对槽的正常生产和以后的槽寿命有着直接影响，燃料焙烧法具有加热均匀、升温速度容易控制、对扎缝和边部扎固糊的焙烧充分等优点，是一种非常有前途的新焙烧方法。而燃烧器的设计是燃料焙烧法的关键技术之一，在实验的基础上开发出一种矩形高速燃烧器，并对该燃烧器的结构进行了优化，研制的矩形顺具有点火容易、燃烧稳定、负荷调节比大燃烧完全度高、噪音低等特点，能完全满足铝电解槽燃料焙烧方法的要求，它的研制成功为铝电解槽焙烧新技术的应用提供了技术保证。     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system

The human adenovirus type 5 E1A, a tumor- suppressor gene[1], codes for two major related proteins of 243 amino acids (12S) and 289 amino acids (13S) by al-ternative splicing in two exons[2]. Studies have been shown that E1A can regulate expression of many genes and cell cycle[3]. Both in vitro and in vivo experiments indicated that E1A could induce tumor cells differentia-tion, convert tumor cells into an epithelial phenotype, in-hibit tumor cell growth and metastasis and strongly en-ha…     

Building of hFⅨ transgenic mice by spermatogenic cells

Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

废旧锌锰电池的回收利用

分析了我国废旧电池的回收利用现状。阐述了废旧电池对生态环境造成的危害和回收利用废旧电池的重大意义。介绍了回收利用废旧锌锰电池的工艺流程和实验原理。在现有的实验条件下,初步研究了利用废旧锌锰电池制备高锰酸钾、氯化锌、氯化铵等物质的实验方法;分析讨论了制备高锰酸钾的各种影响因素,如反应物的量、反应温度等。     

到达场列车反向接入在车站改造中的应用

根据车站改造充分利用既有设备和节省投资的原则设计出到达场列车全部反向接入的方案,并就因全部反接而在驼峰咽喉处产生的交叉干扰进行了详细的讨论,最后提出用计算机辅助行车的方法实现进路自动设置的构思,说明了此方案在实际运用中的可行性.