黄连素调节eNOS/NO保护软脂酸诱导的HUVECs损伤作用机制研究

目的研究黄连素对软脂酸诱导人脐静脉内皮细胞(HUVECs)损伤的保护作用,并初步探讨其作用机制.方法采用500μmol/L软脂酸培养HUVECs 24 h,建立内皮细胞损伤模型,MTT法观察黄连素对细胞存活率的影响;检测细胞培养上清液中一氧化氮(NO)含量;RT-PCR法检测内皮型一氧化氮合酶(e NOS)mRNA水平;Western blot方法检测e NOS和磷酸化e NOS蛋白的表达.结果与对照组比较,软脂酸组细胞存活率降低(P0.05),培养液中NO含量下降(P0.05),细胞内e NOS mRNA水平、e NOS及磷酸化e NOS蛋白表达水平均显著下降(P0.01,P0.05).与软脂酸组比较,黄连素组细胞存活率增加,培养液中NO含量明显提高(P0.05),细胞内e NOS mRNA水平和磷酸化蛋白表达水平均显著提高(P0.01,P0.05).结论黄连素对软脂酸引起的血管内皮细胞损伤有显著的保护作用,并可能与上调e NOS、促进NO生成有关.     

高效液相色谱法测定地塞米松棕榈酸酯注射液中的有关物质

为建立高效液相色谱法测定地塞米松棕榈酸酯注射液(乳剂)中的有关物质和含量检测的有效方法,采用ODS-C18色谱柱(4.6×250 mm,5μm),流动相为乙腈:水(梯度洗脱),流速1.0 mL·min-1,检测波长240 nm,柱温50℃。结果使有关物质地塞米松与主峰得到良好分离,分离度大于2,在0.25~16.0μg/mL范围内线性关系良好。地塞米松浓度与峰面积之间呈现良好线性关系,r2=0.999 9(n=8),回收率均在84.7%~97.9%之间,RSD(%)小于2.0%。地塞米松棕榈酸酯在20.04~400.8μg/mL间呈现良好线性关系,r2=0.999 3(n=6),回收率均在99.2%~100.9%之间,RSD(%)小于2.0%。该方法简便、准确、专属,可用于该注射液的质量控制。     

Improved photostability of Vitamin A palmitate originating from self-assembled supramolecular gels

Retinyl palmitate(RP) is a derivative of Vitamin A and widely applied as the active component in the fields of cosmetic and pharmaceutics.RP can easily lose its physiological activity under UV irradiation due to its photosensitivity.In this work,it was found that the activity of RP remained 75% after 80 min of UVA irradiation when RP was entrapped in supramolecular gels formed by self-assembly of sorbitol-based gelators.By contrast,the activity of RP in conventional hydroxyethyl cellulose gels only remained 10% under the same conditions.These results showed that the supramolecular gels exhibited a significant protective effect on the photostability of RP.UV spectra of RP in supramolecular gels and corresponding solutions showed no distinct differences,indicating no change of the physicochemical properties of RP.The images of field-emission scanning electron microscopy and fluorescent optical microscopy suggested that the protection by supramolecular gels on the photostability of RP may be attributed to the three-dimensional network structure formed by the self-assembly of the gelators,which reduced the molecular collisions and the degradation process of the photoactivated RP.The present results showed that this protection can be influenced by the structures and the concentration of gelators as well as by the solvent composition.     

固定化脂肪酶催化合成棕榈酸异辛酯的反应器制备研究

开发了填充床式固定化酶反应器合成高档化妆品原料棕榈酸异辛酯的生产工艺，以棕榈酸，异辛醇为原料，使用实验室自制的固定化假丝酵母脂肪酶99-125为催化剂。并对反应器的固定化酶用量，底物浓度，循环流速，填充柱径高比等操作参数以及稳定性进行了研究，反应12h最终的酯化率可达97%，固定化酶使用31批以后，12h酯化率仍然可以达到93%。     

相转移催化合成蔗糖酯：（Ⅲ）蔗糖棕榈酸酯的合成

在合成蔗糖棕榈酸酯中采用相转移催化的基本原理和方法．考察了反应时间、反应温度、原料的不同配比和催化剂用量对蔗糖棕榈酸酯重量产率的影响．得出反应时间为３ｈ，反应温度为８５～９０℃，原料棕榈酸甲酯和蔗糖的摩尔配比为１．０：２．５，催化剂用量为棕榈酸甲酯摩尔数的０．０８～０．１０时，其蔗糖棕榈酸酯产率是８８．３０％．产物经纯化后测定，所含蔗糖棕榈酸单酯与棕榈酸甲酯分子比为０．７７４０：１．００００。     

Green Synthesis of Wax Ester by Immobilized Lipase

1 Results Enzyme catalysis is most attractive for the synthesis and modification of biologically relevant classes of fine organic compounds, which are difficult to prepare and to handle by conventional means[1]. In this study, commercial immobilized lipase from Candida antarctica (Novozym 435) was used in the preparation of fine organic compound with excellent properties and application as raw material for cosmetic formulation - oleyl palmitate. The effect of various reaction parameters were optimized c...     

基于UFLC-Q-TOF-MS/MS技术的盐酸克林霉素棕榈酸酯分散片杂质的研究

采用UFLC-Q-TOF-MS/MS技术,在正离子模式下,根据各物质的质谱裂解规律,研究鉴定出盐酸克林霉素棕榈酸酯分散片中16种杂质,其中克林霉素九酸酯、克林霉素十酸酯、克林霉素十一酸酯、克林霉素十三酸酯为首次检到,本研究为其质量监控提供了实验依据。     

四特酒生产中三种高级脂肪酸乙酯初探

主要研究了四特酒两种原料(高粱与大米)在发酵、蒸馏过程中3种高级脂肪酸乙酯含量的变化。发酵前期,这3种高级脂肪酸乙酯的含量随发酵时间的增加而增加;发酵过程向后推移,其含量有波动;发酵终了,酒醅中这3种酯的含量次序为油酸乙酯>棕榈酸乙酯>亚油酸乙酯。馏出时3种酯的浓度顺序为棕榈酸乙酯>油酸乙酯>亚油酸乙酯。     

产物作溶剂体系固定化酶催化合成棕榈酸异辛酯

以产物作为溶剂,采用固定化脂肪酶催化合成棕榈酸异辛酯。考察了溶剂用量、底物用量比、水含量、水活度对反应的影响以及固定化酶的使用寿命。研究结果表明,最适溶剂量为每g棕榈酸3 mL棕榈酸异辛酯,最适醇酸物质的量比为1.3∶1;同时体系中水含量越高反应转化率越低,使用MgC l2饱和盐溶液可将反应体系的水活度控制在0.33左右,可使反应转化率增加20%左右。以1 g棕榈酸、0.67 g异辛醇、0.12 g固定化脂肪酶和3 mL棕榈酸异辛酯组成的反应系统在40℃条件下敞口反应24 h,酯化率可达97.5%,固定化酶连续反应30批后酯化率仍能达到95%以上。     

棕榈油的综合开发与利用研究（二）

本文研究了从棕榈油中获得棕榈酸，再借助催化剂与不同的醇反应合成棕榈酸酯，确定了最适宜的反应条件，并研究了棕榈酸合成其他不同类型的表面活性剂，为棕榈油的综合开发与利用提供了科学依据。