Nucleotide analogues as probes for DNA polymerases

Transmission of the genetic information from the parental DNA strand to the offspring is crucial for the survival of any living species. In nature, all DNA synthesis in DNA replication, recombination and repair is catalyzed by DNA polymerases and depends on their ability to select the canonical nucleobase pair from a pool of structurally similar building blocks. Recently, a wealth of valuable new insights into DNA polymerase mechanisms have been gained through application of carefully designed synthetic nucleotides and oligonucleotides in functional enzyme studies. The applied analogues exhibit features that differ in certain aspects from their natural counterparts and, thus, allow investigation of the involvement and efficacy of a chosen particular aspect on the entire complex enzyme mechanism. This review will focus on a depiction of the efforts that have been undertaken towards the development of nucleotide analogues with carefully altered properties. The different approaches will be discussed in the context of the motivation and the problem under investigation.Received 16 March 2005; received after revision 5 May 2005; accepted 8 June 2005     

基于复制技术的远程数据汇总软件设计

数据复制是网络环境下分布式数据库应用的基本问题．结合财务数据汇总系统提出并设计了一个可以实现跨Internet数据复制的解决方案．该方案设计简单,实现容易,可以满足财务数据汇总要求．实验表明,该方案是可行的、实用的．     

Pocket PC与SQL Server数据库同步移动数据的研究

研究了如何使用合并复制来实现Pocket PC的SQL CE（Microsoft SQL Server CE）数据库与PC服务器的SQL Server 2000数据库间的数据同步与数据存取交换,并给出了示例代码。。     

Dynamic replication strategies in data grid

Dynamic data replication is a technique used in data grid environments that helps to reduce access latency and network bandwidth utilization. Replication also increases data availability thereby enhancing system reliability. In this paper we discuss the issues with single-location strategies in large-scale data integration applications, and examine potential multiple-location schemes. Dynamic multiple-location replication is NP-complete in nature. We therefore transform the multiple-location problem into several classical mathematical problems with different parameter settings, to which efficient approximation algorithms apply experimental results indicate that unlike single-location strategies our multiple-location schemes are efficient with respect to access latency and bandwidth consumption, especially when the requesters of a data set are distributed over a large scale of locations.     

AcMNPV p95基因一段339 bp序列在病毒复制中的作用

杆状病毒苜蓿银纹夜蛾核型多角体病毒(AcMNPV)的p95基因编码的P95蛋白是病毒核衣壳的组成部分,也是虫体经口感染相关因子PIF(Per os Infectivity Factor)复合体的组成部分.前期研究表明,完整的P95蛋白对病毒的复制是非必需的,其中一段450bp的序列可以部分弥补p95基因的作用.本研究将p95基因一段339bp的片段(Core339,AcMNPV基因组位置:69 576~69 914bp)以正反两个方向分别插入到p95基因敲除的AcMNPV基因组中多角体位点的polyA之后,构建了两株重组病毒vP95K/EGFP/339+和vP95K/EGFP/339-.该两株病毒能在Sf9细胞中正常复制,复制水平与p95基因正常的AcEGFP相当,但复制速度较慢.重组病毒感染细胞的Western blot分析未检测到P95蛋白的表达.这一结果表明P95蛋白的缺失对病毒在细胞中的复制没有显著影响,但核心片段Core339对于病毒复制具有关键作用.     

一步成像虹全息图金属模复制技术

利用一步成像法拍摄浮雕型彩虹全息图母版 ,重点讨论了将其转印到金属镍模上的复制工艺 ,它既可作为模压全息图的全息母版 ,又具有独特的推广应用前景     

一种动态数据网格副本创建策略设计与仿真

数据网格提供了地理分布的大量共享数据资源,然而互联网的高访问延迟降低了数据访问的效率,创建副本是一个有效的方法,而在何处放置副本是一个具有挑战性的问题.从应用环境和用户访问特征出发,讨论了副本创建位置、粒度和时机,提出了一种基于副本共享组的副本创建模型,可以获得优化的副本创建位置,构造了一个副本创建代价函数,由系统的传输速率、拓扑结构和用户访问特征共同决定.并给出了确定副本创建位置的有效算法.分析和仿真实验表明,自适应副本管理方案具有动态性、适应性和可扩展性,能较好地适应数据网格的特性,可以有效降低访问延迟,提高数据访问效率.     

Replication pattern of double minutes derived from an insect cell line

Summary The DNA replication pattern of double minutes derived from an established cell line ofAedes albopictus is described. Although the vast majority of double minutes replicate semiconservatively once during the S phase, some double minutes appear to exhibit different pattern(s). Two theories are suggested as possible explanations of our findings.This work was supported by a grant from the Whitehall Foundation, Inc. awarded to A.B.M.     

番茄DNA复制因子RFC1在减数分裂重组中的功能研究

减数分裂是真核生物有性生殖所必需,前期研究表明拟南芥DNA复制因子RFC1在减数分裂重组中具有重要作用,但其同源基因在其他物种中的减数分裂或其他功能还未有报道.为了检验番茄RFC1在减数分裂中的功能,我们建立了减数分裂特异RFC1~(RNAi)转基因番茄,发现其花粉活性显著降低,而且减数分裂异常.利用DAPI染色观察染色体形态,发现RFC1~(RNAi)减数分裂细胞在终变期和中期Ⅰ形成多价体,表明重组异常.DNA双链断裂指示蛋白γH2AX在RFC1~(RNAi)粗线期染色体上分布的保留滞后,进一步支持RFC1~(RNAi)中的重组异常.拟南芥和番茄分别属于真双子叶植物中的两个不同大支,蔷薇类和菊类,因此本文的结果也表明DNA合成基因RFC1在进化距离很远的不同植物物种中的功能相对保守.