极端嗜热微生物分子伴侣蛋白研究与嗜热功能蛋白质的规模化制备技术研究

极端微生物是丰富的资源宝库,有着巨大的生物技术开发前景。本文从特殊功能蛋白质的发现与表征、结构与功能及潜在性应用．以及特殊功能蛋白质的规模化制备技术两个方面,对极端微生物的资源开发与利用进行了探讨。重点介绍了极端嗜热古菌Pyrococcus furiosus分子伴侣蛋白系统的一些研究,并以P．furiosus胞外α-淀粉酶PFA为例,对工业生物技术领域重组蛋白质的规模化制备技术进行了讨论。     

分布式虚拟环境中时间一致性研究

在分布式虚拟环境中,为了给用户造成逼真的和符合真实世界的时空感受,需要解决时空不一致的问题。要做到时空一致,时间一致是关键,本文主要对分布式虚拟环境中时间一致性进行了研究,介绍了几种时间同步的方案,并结合实际应用,给出了实验的结论。     

基于深腾1800机群系统的分子动力学并行仿真研究

介绍了分子动力学并行仿真计算的软硬件环境,分析了现有的几种并行算法,确定采用区域分解法作为并行算法,并在此基础上提出了基于区域二次划分的分子动力学并行仿真算法.另外,阐述了原子链、原子近邻表和原子亲属表的概念,提出了基于永久序号的消息传递策略.最后,设计了分子动力学并行仿真程序,并分别在1、2、3、4台结点机上进行了实验,运行结果表明:加速比随着结点数的增加而增加,并行效率虽略有下降但都在87.5%以上,并行效率并没有随着结点数的增加有明显的降低,说明并行程序具有很好的扩展性.     

有机物分子量分布特点对BAF-O3-BAC净水工艺的评价

采用曝气生物滤池(BAF)、气浮和臭氧生物活性炭(BAC)联用技术对太湖原水进行试验.有机物分子量分布测定结果表明:曝气生物滤池单元对分子量小于0.5 kD(道尔顿)的有机物去除率最高,其次是分子量介于1～3 kD的有机物;气浮单元对分子量大于100 kD的有机物去除率最高;臭氧氧化单元对分子量大于3 kD的有机物去除率较高,而对于小于3 kD的有机物不但不能去除,反而有所增加;生物活性炭单元对分子量小于10 kD的有机物均能有效去除,分子量越小,去除率越高.综合评价认为:曝气生物滤池、气浮和臭氧生物活性炭联用技术处理微污染水源水的净水工艺是合理的.     

Gas-liquid phase coexistence and finite-size effects in a two-dimensional Lennard-Jones system

The gas-liquid phase coexistence in a two-dimensional Lennard-Jones system is investigated using Maxwell construction method together with molecular dynamics simulations.The results of phase coexistence in different truncations of the potential are compared with data obtained from the literature,and the corresponding critical properties calculated.The crossover from Ising-like to mean field behavior is observed and confirmed as the temperature approaches the critical point from below.Performing simulations on systems with different sizes,we find that a finite size effect is more significant than those shown in most of the previous results,and a lower critical temperature is obtained when the full extent of this finite size effect is considered.     

水溶性共轭聚合物PPEASO3分子量的粘度法估测

利用稀溶液粘度法对所合成的水溶性共轭聚合物PPEASO3的分子量进行了估测.先通过含0.1M NaNO3的PPEASO3水溶液的粘度测量数据求出其特性粘数[η],再利用Mark-Houwink方程[η]=KMαη和有关文献中的三种参考聚合物的K、α值计算得到聚合物的粘均分子量Mη,最后取其平均值得到PPEASO3的粘均分子量为Mη≈150KD.     

单片机总线上多时钟域下数据传递的可靠性研究

对单片机总线上多时钟域下数据传递的可靠性进行研究，阐述了使用寄存器锁存电路、多级锁存电路2种方法解决多时钟域下数据传递可靠性时存在的问题，提出了采用4级锁存并判断跳变沿的方式能确保多时钟域下数据可靠传递的方法，具有很高的应用价值．     

Tunable water-based lubrication behavior of alkyl- and fluoroalkyl-silanes

In this paper,we report the tribological properties of self-assembled molecular(SAM) films of fluoroalkylsilanes and non-fluoroalkylsilanes,with different chain-lengths,adsorbed on Si substrate surfaces by covalent bonds.The SAM films were characterized using a universal ball-disk experimental tester in aqueous solutions.The substrate surface was examined by X-ray photoelectron spectroscopy(XPS),and the SAM films adsorbed on the Si surfaces were inspected by contact angle measurements and XPS.Lubrication studies revealed that several kinds of fluoroalkylsilanes had similar friction coefficients;the small differences were attributed to the chain flexibility.In contrast,differences in the aqueous lubrication properties of SAM films of non-fluoroalkylsilanes were clearly identified.It is suggested that substitution with fluorine atoms and the surface affinities of fluoroalkylsilanes contributed to redistribution of surface changes,causing variations in lubrication behaviors.     

The complete mitochondrial genome of the Chinese Sika deer (Cervus nippon Temminck, 1838), and phylogenetic analysis among Cervidae,Moschidae and Bovidae

The complete mitochondrial genomes of the Sichuan Sika deer (Cervus nippon sichuanicus) and the South China Sika deer (Cervus nippon kopschi) were determined. Their sizes were 16,429 and 16,428 base pairs, respectively. The similarity of the mitochondrial DNA genome among subspecies of the Chinese Sika deer was calculated, and the values estimated from complete nucleotide sequences and entire amino-acid sequences were 98.4–99.9% and 99.7–99.9%, respectively. Molecular phylogenetic analyses were conducted using maximum parsimony and Bayesian inference methods based on the sequences of 12 concatenated heavy-strand encoded protein-coding genes. The consensus of phylogenetic trees supported the monophyly of Moschidae, Cervidae and Bovidae, and placed Moschidae as a sister group to Cervidae/Bovidae. The relationships among Chinese Sika deer inferred from the phylogenetic analysis were inconsistent with subspecies designations and the present geographic distribution. Our complete mitochondrial genomes will be available as important and basic data for phylogenetics, breeding, genetics and conservation in Cervidae.     

分子生物学实验教学改革的探讨

针对近年来高校对本科生开设分子生物学实验中出现的三种矛盾,根据多年的实验室建设和实验教学经验,文中对分子生物学实验课的开设提出4条革新意见,为前进中的本科生生物类实验课的改革方面提供参考。