Dissection of a novel molecular determinant mediating Golgi to trans-Golgi network transition

Two major functions of the Golgi apparatus (GA) are formation of complex glycans and sorting of proteins destined for various subcellular compartments or secretion. To fulfill these tasks proper localization of the accessory proteins within the different sub-compartments of the GA is crucial. Here we investigate structural determinants mediating transition of the two glycosyltransferases β-1,4- galactosyltransferase 1 (gal-T1) and the α-1,3-fucosyltransferase 6 (fuc-T6) from the trans-Golgi cisterna to the trans-Golgi network (TGN). Upon treatment with the ionophore monensin both glycosyltransferases are found in TGN-derived swollen vesicles, as determined by confocal fluorescence microscopy and density gradient fractionation. Both enzymes carry a signal consisting of the amino acids E₅P₆ in gal-T1 and D₂P₃ in fuc-T6 necessary for the transition of these glycosyltransferases from the trans-Golgi cisterna to the TGN, but not for their steady state localization in the trans-Golgi cisterna. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. Received 30 July 2008; received after revision 17 September 2008; accepted 29 September 2008     

用于锋电位分类的二阶差分表示法

针对锋电位在相似度高及存在大量叠加锋电位时分类结果不理想的问题，提出了一种新的锋电位特征表示方法一二阶差分表示法．该方法对锋电位波形求取二阶差分，以二阶差分序列作为锋电位的特征信息，形成新的样本向量进行分类．该方法对Wave_clus中不同信噪水平的数据分别进行了实验．实验表明，该方法描述了锋电位波形在各个时刻的波形趋势，在一定程度上能够扩大不同类型波形之间的差异性．将此方法用于锋电位分类，尤其是叠加锋电位分类，可以提高分类准确率，并且可以有效的避免噪声的干扰．     

生甘草与大叶海藻配伍后部份药理实验研究

本文观察了生甘草、大叶海藻及其合剂对小鼠的急性毒性作用及镇痛作用,对离体蛙心,家兔离体小肠运动的影响,为甘草与大叶海藻的伍用提供了部份实验数据。     

环境振动数据的周期性检验

本文陈述了环境振动数据的特点及由此引起的周期性检验的困难，分析了不同带宽法中引起加速度谱图幅值差异的主要因素及其影响，提出了周期性检验切实可行的公式，并作出了评价．本文最后用实例说明了公式的具体应用．     

用单板机对供电电网谐波测试

本文阐述一种供电电网谐波测试方法。采用Z—80单板机可以迅速得到供电电网各项谐波参数。文中介绍了FFT变换程序、重排序的新方法和三角函数查表法等。采用上述措施后,该装置具有高精度和高速度,为制造各种谐波分析仪和实现供电电网抑制谐波的实时控制提供了可能性。     

应用密闭微波辅助提取技术提取玄参中玄参苷

应用密闭微波辅助提取法提取玄参中有效成分玄参苷，考察了微波提取条件（包括溶剂、样品粒径、提取时间、固-液质量体积比和提取次数等）对玄参中提取玄参苷收率的影响，结果表明：溶剂为70％乙醇，玄参的粒径为180目，提取时间为10min，料液比为1：20及提取次数为2次时，玄参苷的提取率最佳。并且与常压微波辅助提取法、超声波提取法及索氏提取法进行了比较，本法具有快速、高效、绿色环保的优点。     

不计评价指标权重值时的综合评价

多指标系统的综合评价，一直以来都必须确定评价指标的权重值，但采用的这些方法经常带有较大的主观随意性。采用同异反联系数及其态势排序分析技术，可以在不计因素权重值的情况下，进行系统综合评判。     

求凸壳顶点的一种算法

提出了一种求平面有限点集凸壳顶点的算法,并分析出该算法的时间复杂性是线性次乘法和O(nlogn)次两个数的比较。     

Multi-objective dynamic detection of seeds based on machine vision

An approach to inspecting massive numbers of moving seeds was studied based on the techniques of dynamic inspection and machine vision. A progressive scanning CCD camera with external trigger function was used for real-time capture of dynamic images of seeds. The methods based on R channel of RGB (Red, Green and Blue) and region-dependent segmentation were adopted to reduce the data size of image processing and improve the efficiency of seeds inspection. All the seeds were sorted into four grades according to their morphological characteristics, such as surface area, perimeter, major axis, minor axis, circularity and eccentricity. The detection experiments indicated that the eligible ratio of the classifications was about 81.90% by this real-time inspection system.     

纸币清分图像识别算法研究

针对国产纸币清分机在图像识别技术上,存在挑残能力弱和分类一致性差的缺点,提出了3种基于图像识别技术的算法。通过对采集的图像数据,采用五线找点算法对纸币大小进行判定纸币的面额;根据加权灰度值提取纸币图像的特征区域判正反;采用网格划分法统计背景点与纸币图像点的数量关系判定纸币的残缺率。