适用于膜片钳技术的细胞分离及培养技术的研究进展

生物细胞膜片钳技术自八十年代问世以来,已经在电生理领域得到了很大的发展,但由于细胞分离及培养技术的原因,时至今日此项技术手段仍未普及。本文综述了膜片钳技术和组织培养,主要内容包括钳技术,组织培养的历史发展以及适用于膜片钳技术的神经细胞,心肌细胞,骨骼肌细胞等的分离培养方面的进展及前景展望。     

Basic equations of channel model for underground coal gasification

The underground coal gasification has advantages of zero rubbish, nonpollution, low cost and high safety. According to the characteristics of the gasification, the channel model of chemical fluid mechanics is used to set up the fluid equations and chemical equations by some reasonable suppositions in this paper, which lays a theoretical foundation on requirements of fluid movement rules in the process of underground coal gasification.     

城市微区移动无线信道的特征分析与建模

对城市微区移动无线信道的概念及其研究方法进行了系统分析和介绍，在此基础上分析研究了多径色散传播信道的基本特征以及信道的建模方法。     

海陆战场环境的实时视景仿真

海陆战场环境是登陆作战的主要战场环境。本文研究了海浪的数学模型、离散化过程、海浪可视化中的实时性问题、地形生成、海陆拼接、两栖车辆海上姿态仿真、BSP技术和多通道视景技术，并将这些技术成功应用于海陆战场环境仿真中。     

解广义特征值问题的半分法

本文提出了一个解广义特征值问题的方法,即用半分法确定近似特征值,用改进的Rayleigh商迭代校正近似特征值,同时求出相应的特征向量。理论证明它是一个收敛的方法。     

一种空时盲RAKE接收机

多用户检测和阵列天线是直扩码分多址系统中两种减轻多址干扰的关键技术 ,将两者结合起来的空时处理能大大提高系统的容量和性能。设计了一种空时盲RAKE接收机。研究结果表明 ,这种空时盲RAKE接收机具有较强的抑制多址干扰以及克服多径效应和“远近”效应的能力 ,并且能快速收敛。     

Down-regulation of sodium current in chronic heart failure: effect of long-term therapy with carvedilol

Evidence has accumulated recently about the importance of alterations in Na⁺ channel function and slow myocardial conduction for arrhythmias in the infarcted and failing heart. The present study tested a hypothesis that Na⁺ current (I_Na/C) density decreases in chronic heart failure (HF) and that Na⁺ channel (NaCh) functional density can be restored by long-term therapy with carvedilol, a mixed α- and β-adrenergic blocker. Studies were performed using a canine model of chronic HF produced in dogs by sequential intracoronary embolizations with microspheres. HF developed approximately 3 months after the last embolization (left ventricle, LV, ejection fraction = 28 ± 1 %). Ventricular cardiomyocytes (VCs) were isolated enzymatically from LV mid-myocardium, and I_Na was measured by whole-cell patch-clamp. The maximum I_NA/C was decreased in failing (n = 19) compared to normal (n = 12) hearts (33.1 ± 1.6 vs 48.5 ± 5.1 pA/pF, mean ± SE, p < 0.001). The steady-state inactivation and activation of I_Na remained unchanged in failing compared to normal hearts. Long-term treatment with carvedilol (1 mg/kg, twice daily for 3 months) normalized I_Na/C in dogs with HF. I_Na/C in HF dogs (n = 6) treated with carvedilol was higher compared to that of non-treated HF dogs (n = 6) (49.4 ± 0.9 vs 29 ± 4.8 pA/pF, p < 0.007). In vitro culture of VCs of failing hearts for 24 h did not restore I_Na/C. However, I_Na/C was partially restored when VCs were incubated for 24 h with BAPTA-AM, an intracellular Ca²⁺ buffer. Thus, we conclude that experimental chronic HF in dogs results in down-regulation of the functional density of NaCh that can be restored by long-term therapy with carvedilol. The mechanism of NaCh down-regulation in HF may be linked to poor Ca²⁺ handling in this stage of disease. Received 4 June 2002; received after revision 1 July 2002; accepted 17 July 2002 RID="*" ID="*"Corresponding author.     

一种对抗强单音干扰的短波OFDM信道均衡机制

短波信道使用的频率范围较窄,频谱资源十分紧张,会出现大量的强单音干扰。因此针对存在强单音干扰的短波信道,提出了一种高效、简洁的信道均衡机制,该机制通过干扰抑制、信道估计、维纳插值等算法的有机结合,首先对接收到的信息序列硬判决,基于干扰门限对大幅度干扰进行抑制;其次,根据最小平方准则估计出导频子载波处信道传输函数;最后使用二维维纳插值工具,利用导频符号之间的相关性,计算出数据子载波处信道传输函数。并以强单音干扰下短波正交频分复用(OFDM)系统为例,对所提出的机制进行了仿真验证,结果表明,与其它均衡机制比较,所提出机制降低了接收端符号误码率,有效地提高了通信可靠性。     

基于载干比测量的动态信道分配

讨论了基于载干比测量的动态信道分配方案。从理论上分析了干扰自适应的动态信道分配（ＩＡ－ＤＣＡ）的特点,并给出了它所能达到的极限系统容量。通过对固定信道分配（ＦＣＡ）,话务量自适应动态信道分配（ＴＡ－ＤＣＡ）和ＩＡ－ＤＣＡ的仿真,比较了各信道分配方案的含量,理论分析和仿真实验均说明,基于载干比测量的动态信道分配方案具有明显的容量优势,而且容量的改善和系统中所有连接的平均载干比测量的动态信道分配方案具     

采用迭代处理的多用户联合检测和译码方案

将迭代（Turbo）处理的思想应用于多用户检测和译码中,通过软输入软输出（SISO）算法使二者能利用对方在上一轮运算中的比特对数似然比（LLR）信息,用迭代递归的方法逐渐逼近单用户性能,减少多址干扰的影响。仿真结果给出了迭代处理方法在不同信噪比条件下的比特误码率性能,证明了该方法确实可提高数据接收质量。