Adrenocortical metabolism and plasma corticosterone in soman intoxicated rabbits

Summary The organophosphate neurotoxin soman produced impairments in adrenocortical RNA and protein metabolism. Fasciculate and reticular cell RNA and protein contents were supporessed with sublethal to acutely lethal dosages (20, 30 and 40 g/kg, s.c.) during the acute excitatory phase of intoxication and at 6–8 h post injection. All three dosages produced ca 90% inactivation of plasma cholinesterase. A transient elevation of plasma corticosterone occurred with 20 g/kg soman whereas there was a protracted increase with 30 g/kg. Corticosterone was not significantly elevated with 40 g/kg, but death occurred at 13±4 min. Thus, the magnitude and/or nature of soman-induced metabolic impairments does not appear to prevent adrenal activation.Supported by US Army Medical Research and Development Command Contract DAMD 17-81-C-1202.     

Morphological,biochemical and molecular changes during ectomycorrhiza development

Summary An ectomycorrhiza, a specialized root organ, is the result of a complex interaction leading to a finely-tuned symbiosis between a plant and a compatible ectomycorrhizal fungus. Ultrastructural observations combined with cytochemical and biochemical studies reveal that structural and metabolic changes in the symbiont cells lead to the final phenotype of the active ectomycorrhiza. In the present review these changes are interpreted as changes in gene expression and discussed within the context of ectomycorrhiza development. Recent genetic data indicate that the continued vegetative growth of the ectomycorrhizal hyphae and the root tissues, and their ability to switch to symbiotic organ formation, is basically controlled by developmentally critical genes. The activity of these symbiotic genes during the differentiation of ectomycorrhizas is associated with extensive changes in the concentration of particular polypeptides and protein biosynthesis. The present state of knowledge about the developmental biology of ectomycorrhizas allows only speculation about the events during their development.Puisant mes forces aux sources des galaxies En buvant la sève des arbres M. Jonasz     

山羊血液蛋白多态性的研究动态

综述了山羊血液蛋白多态性的研究动态,较详细地介绍了山羊血液生化遗传标记的研究现状及其在山羊育种中的应用,并对其应用前景作了展望     

莲子叶蛋白体的类型,来源与发育

莲子叶于发育的１０￣２８ｄ,以不同速率在叶肉细胞中大量积累贮存蛋白,并以不同方式组装许多形态各异、结构不同的蛋白体。按其来源与发育的特点可分４种类型：①粗面球状蛋白体;②光面球状蛋白体;③杯状蛋白体;④淀粉蛋白体。发育顺序是①,②类先形成,③,④类后发生;②类的发育贯穿整个贮存蛋白的积累期,比较了莲与其他植物蛋白体的异同。     

细胞分裂素对大豆叶片衰老过程中蛋白激酶基因表达的影响

利用真核生物催化丝氨酸／苏氨酸残基磷酸化的蛋白激酶ＶＩ和Ⅷ保守区设计兼并引物,进行ＲＴ－ＰＣＲ扩增反应,研究大豆叶片衰老过程中蛋白激酶基因表达的变化及外源细胞分裂素处理的作用。结果表明人工合成的细胞分裂素－６ＢＡ预处理很可能在转录水平上正向调节一些蛋白激酶基因的表达,而诱导衰老处理则起负调控作用。     

黄旗海产卤虫(Artemiapathenogenetica)成虫干片蛋白质、脂肪酸、氨基酸和矿物质的分析

对黄旗海产卤虫（Ａｒｔｅｍｉａｐａｔｈｅｎｏｇｅｎｅｔｉｃａ）成虫干片的蛋白质、脂肪酸、氨基酸和部分矿物质进行了初步分析．结果表明,粗蛋白含量为５４．１４％,脂肪酸中亚麻酸的含量为１５．０７％,蛋白质中的１７种氨基酸和部分矿物质的含量也很丰富,由此说明从盐碱湖中开发利用卤虫资源,使之成为新的食品源和优质饲料有着广泛的前景．     

阳离子反胶团体系提取蛋白质

用阳离子反胶团体系分别对４种蛋白质进行反胶团提取的试验和考察,确定ＰＨ值,离子强度,表面活性剂浓度和助溶剂等影响提取的主要因素。     

超滤法分级和纯化蛋白质的现状和前景(英文)

超滤法具有高产率、低成本、容易放大等显著优点,但其低选择性一直限制其在生物蛋白制品的分级和纯化中的应用。本文总结和分析了超滤法用于蛋白质分级和纯化的近期研究成果,对其低选择性的原因进行了分析,提出并论证了提高超滤选择性的技术途径。     

Molecular evidence for increased hematopoietic proliferation in the spleen of the b/b laboratory rat

The splenomegaly and the appearance of a significant number of CFU-E (erythroid colony-forming units) and BFU-E¹ (erythroid burst-forming units) in the Belgrade laboratory rat (b/b) spleen prompted us to analyse further the molecular evidence for increased hematopoietic proliferation in the b/b spleen. Messenger RNAs (mRNAs) specific for globins, proteins for iron transport and deposition and the band 3 protein were used in rat erythropoietic tissues as markers for proliferation and erythroid differentiation. In the b/b spleen, all mRNAs analysed display an erythroid-specific pattern of expression. This analysis also revealed an enhanced level of mRNA for ferritin in the +/b spleen, whereas erythrocyte-specific mRNA production was normal.     

Studies of a key protein in the mechanism of the excitation-contraction coupling process of frog skeletal muscle,using phenylglyoxal

The excitation-contraction (E-C) coupling process in single twitch fibres from frog toe muscle was inhibited selectively by phenylglyoxal (PGO), a specific guanidyl modifying reagent. A new protein (31.5 kDa), which has PGO-binding ability and seems to play a key role in the E-C coupling process, was solubilized from transverse tubule membrane-junctional sarcoplasmic reticulum complexes (TTM-JSR) of frog skeletal muscles, using¹⁴C-PGO. The monoclonal antibody against this protein applied extracellularly inhibited the E-C coupling process of the single fibres. This protein appears to constitute the very first step of input for E-C coupling. It is considered to behave as an indispensable part of an electrometer to measure membrane potentials. Therefore, the name electrometrin is suggested for the new protein.