Prostate cancer targeted MRI nanoprobe based on superparamagnetic iron oxide and copolymer of poly（ethylene glycol） and polyethyleneimin

Methoxyl poly （ethylene glycol） （mPEG-OH） was successfully grafted onto branched polyethyleneimine （hy-PEI） to yield a water soluble graft copolymer mPEG-g-PEI. This copolymer may package superparamagnetic iron oxide （SPIO） by ligand exchange. The SPIO weight percentage in the polymer coated nanoparticles was determined to be 55%, the size and zeta potential of nanoparticles was 50 nm and 12 mV respectively. Antibody fixation onto the complex （mPEI-g-PEG-SPIO） surface layer was achieved by activated single chain monoclonal antibody against prostate stem cell antigen （PSCA）. Our study showed that the single chain antibody functionalized nanoprobe （scAbpscA-PEI-g-PEG-SPIO） with a small size can specifically enter the prostate cancer cells, decreasing MRI T2-weighted signal intensity of prostate cancer cells to 44.76%, Our results revealed that the potential of this magnetic nanoparticulate system promised as a novel MRI nanoprobe for early diagnosis of prostate cancer （PCa）.     

性激素对大鼠前列腺腹叶酸性磷酸酶影响的组织化学研究

成年雄性Ｗｉｓｔａｒ大鼠８０只分为５组：即对照组（Ｎ组）、正常用雌激素处理组（Ｎ＋Ｅ２）、阉割组（Ｃ组）、阉割后用雌激素处理组（Ｃ＋Ｅ２组）和阉割后用雄激素处理组（Ｃ＋Ｔ组）。于处理后第１、２、３、６和１２周分５批取材。用酶组化方法显示大鼠前列腺腹叶（ＲＶＰ）酸性磷酸酶（ＡＣＰ）的活性变化。结果表明：Ｎ＋Ｅ２组酶活性明显增强，Ｃ组酶活性逐渐减弱，直到消失，Ｃ＋Ｅ２组和Ｃ组结果相似，仅第２周稍有增加，Ｃ＋Ｔ组酶活性变化不明显，提示ＲＶＰ上皮细胞内ＡＣＰ的活性变化与大鼠体内性激素的含量和平衡状态密切相关。     

泛素连接酶Nedd4调控人前列腺癌细胞的增殖与凋亡

为了研究泛素连接酶Nedd4对肿瘤细胞增殖和凋亡的影响,利用小RNA干扰技术,通过慢病毒包装,侵染前列腺癌细胞DU145,达到下调Nedd4表达的目的.MTT检测表明,下调Nedd4表达可以抑制DU145细胞的增殖;DAPI染色发现,下调Nedd4表达的DU145细胞对星形孢菌素诱导的细胞凋亡更加敏感.这些结果都说明,下调Nedd4的表达不仅可以抑制DU145肿瘤细胞增殖,而且可以促进细胞凋亡.     

烯菌酮对小鼠前列腺癌细胞增殖及其脂质过氧化物含量和抗氧化物酶活性的影响

为研究烯菌酮对小鼠前列腺癌(RM-1)细胞增殖及其脂质过氧化物和抗氧化物酶的影响,实验采用了四唑盐法(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide,MTT)检测了RM-1细胞增殖活力,采用比色法测定了其细胞内抗氧化物酶活性和脂质过氧化物(Maleic Dialdehyde,MDA)含量的改变。结果表明,0.01μmol/L、1μmol/L和100μmol/L三个浓度的烯菌酮作用24和48 h,RM-1细胞增殖活力均明显低于对照组。100μmol/L烯菌酮作用48 h,小鼠前列腺癌细胞中脂质过氧化物含量明显高于对照组及0.01μmol/L和1μmol/L两个浓度组。烯菌酮作用24、48和72 h,RM-1细胞中总超氧化物歧化酶(Superoxide Dismutase SOD)和谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-PX)活性随烯菌酮浓度增加而增加,具有浓度依赖性。0.01μmol/L、1μmol/L和100μmol/L烯菌酮作用24 h时,RM-1细胞中过氧化氢酶(Catalase,CAT)活性明显低于对照组,但随着烯菌酮浓度增加,CAT活性明显增加。100μmol/L烯菌酮作用48和72 h时,RM-1细胞中CAT活性明显高于其他三组。这说明,烯菌酮能抑制RM-1细胞增殖活力,促进其细胞内脂质过氧化物的含量和抗氧化物酶的活性。     

Study of prostate biopsy robot system

A system for prostate biopsy with robot assistance was proposed. The system consists of Motoman robot, needle insertion mechanism, and control software. A experiment was held with this software, and it proved that the whole system is simple, reliable and good application.