棉铃虫单粒包埋型核型多角体病毒诱发草地贪夜蛾细胞株Sf的程序性死亡

棉铃虫单粒包埋型核型多角体病毒（ＨａＳＮＰＶ）能够诱发草地贪夜蛾Ｓｆ细胞株发生早期死亡现象．依据死亡过程的细胞形态变化，ＤＮＡ降解的梯状电泳特征以及对不同抑制剂敏感性的差异，可以初步断定该死亡现象为程序性死亡．早期死亡伴随着病毒ＤＮＡ复制与子代病毒生成的中止．同时发现野生型ＡＣＭＮＰＶ的共感染能够有效地抑制早期死亡的发生，并对抑制作用的可能性机制进行了探讨．     

Ce4+诱导的花生悬浮细胞程序性死亡

应用超薄切片透射电镜观察和DNAladder检测技术,对添加不同浓度Ce4 胁迫诱导的花生悬浮细胞进行观测.结果表明,花生悬浮细胞经1 0mmol/LCe4 胁迫诱导培养7d,细胞凋亡率达57 6%;发生凋亡的细胞呈现典型的细胞程序性死亡特征:核仁消失,染色质凝缩、趋边化;线粒体嵴模糊不清,基质电子密度减少,出现大小不一的空泡,有的线粒体缢缩,或破裂,甚至解体;液泡有的破裂,有的相互融合,有的与内质网连接在一起;内质网槽库膨大,并出现自噬泡;DNA特异性降解,产生大小约200bp及其倍数的片断,电泳形成梯状条带.而对照组细胞核DNA完整,呈大片段,细胞器发达,细胞完整.证明Ce4 能诱导花生悬浮细胞发生程序性死亡.     

细胞的程序死亡研究进展

本文主要讲述了细胞程序死亡(PCD)的基本概述,细胞程序死亡的诱导因素以及近年来细胞程序死亡方面的有关机理。     

细胞凋亡相关因子的研究进展

主要论述了细胞凋亡的特征、细胞凋亡的调控因子及其调控机理,简要说明了细胞凋亡的生物学意义.     

定量解析TPR谱图的计算方法

本文从常用的气-固相还原反应速率模型出发,讨论了定量解析程序升温还原(FPR)谱图,求取还原动力学参数的若干方法.在均匀表面的假设下,采用已见报道及本文提出的方法对CuO-ZnO-Al_2O_3型低变催化剂的TPR谱图进行了解析,得到了相互印证的计算结果.并建立了由实验上易获取的参数估算还原活化能的经验公式.在非均匀表面的前提下,提出了解析了PR谱图的计算方法,并指出了均匀表面计算结果与非均匀表面计算结果之间的内在联系.     

基于FPGA的高性能关节伺服系统控制器实现

提出了一种基于NIOS Ⅱ软核处理器的全数字化的关节伺服系统控制器的设计方案.该方案不仅实现了电机的矢量控制算法、M/T测速算法以及SPWM算法,而且还实现了关节空间的轨迹规划、位置控制和力矩控制.同时在所设计的关节控制器FPGA内部嵌入了CAN控制器软核,实现了与主控计算机的CAN总线接口和关节控制器参数的在线调整.关节内部电流环控制采样频率为20 kHz,而位置环、力矩环的控制采样频率低于1 kHz.实验结果表明:关节系统的定位精度优于0.01°,由6个完全相同的关节构成的空间机械臂(臂长1.8 m),末端定位精度为±2.7 mm,完全满足空间机械臂系统的要求.     

可编程时间控制器的设计

本文采用小规模数字集成电路和存储器ＲＡＭ２１１４组成可实现２４小时制,控制时间可以任意设定,控制精度为一分钟的可编程时间控制器,该控制器具有廉价,可靠性高,实用性广泛等优点。     

The BAG proteins: a ubiquitous family of chaperone regulators

The BAG (Bcl-2 associated athanogene) family is a multifunctional group of proteins that perform diverse functions ranging from apoptosis to tumorigenesis. An evolutionarily conserved group, these proteins are distinguished by a common conserved region known as the BAG domain. BAG genes have been found in yeasts, plants, and animals, and are believed to function as adapter proteins forming complexes with signaling molecules and molecular chaperones. In humans, a role for BAG proteins has been suggested in carcinogenesis, HIV infection, and Parkinson’s disease. These proteins are therefore potential therapeutic targets, and their expression in cells may serve as a predictive tool for such diseases. In plants, the Arabidopsis thaliana genome contains seven homologs of the BAG family, including four with domain organization similar to animal BAGs. Three members contain a calmodulin-binding domain possibly reflecting differences between plant and animal programmed cell death. This review summarizes current understanding of BAG proteins in both animals and plants. Received 21 November 2007; received after revision 17 December 2007; accepted 2 January 2008     

The Penicillium chrysogenum antifungal protein PAF, a promising tool for the development of new antifungal therapies and fungal cell biology studies

In recent years the interest in antimicrobial proteins and peptides and their mode of action has been rapidly increasing due to their potential to prevent and combat microbial infections in all areas of life. A detailed knowledge about the function of such proteins is the most important requirement to consider them for future application. Our research in recent years has been focused on the low molecular weight, cysteine-rich and cationic antifungal protein PAF from Penicillium chrysogenum, which inhibits the growth of opportunistic zoo-pathogens including Aspergillus fumigatus, numerous plant-pathogenic fungi and the model organism Aspergillus nidulans. So far, the experimental results indicate that PAF elicits hyperpolarization of the plasma membrane and the activation of ion channels, followed by an increase in reactive oxygen species in the cell and the induction of an apoptosis-like phenotype. Detailed knowledge about the molecular mechanism of action of antifungal proteins such as PAF contributes to the development of new antimicrobial strategies that are urgently needed. Received 09 August 2007; received after revision 17 September 2007; accepted 19 September 2007     

活性氧与植物细胞程序性死亡

在植物细胞中,线粒体ETC的复合物I和III是ROS产生的主要部位。大量证据表明,ROS可作为一普遍存在的信号分子在胁迫诱导植物PCD过程中起作用,而线粒体处于PCD调控的中心位置。