Patterning the neuronal cells via inkjet printing of self-assembled peptides on silk scaffolds

The patterning of neuronal cells and guiding neurite growth are important for neuron tissue engineering and cell-based biosensors. In this paper, inkjet printing has been employed to pattern self-assembled I₃QGK peptide nanofibers on silk substrates for guiding the growth of neuron-like PC12 cells. Atomic force microscopy (AFM) confirmed the dynamic self-assembly of I₃QGK into nanofiber structures. The printed self-assembled peptide strongly adheres to regenerated silk fibroin (RSF) substrates through charge-charge interactions. It was observed that in the absence of I₃QGK, PC12 cells exhibited poor attachment to RSF films, while for RSF surfaces coated or printed with peptide nanofibers, cellular attachment was significantly improved in terms of both cell density and morphology. AFM results revealed that peptide nanofibers can promote the generation of axons and terminal buttons of PC12 cells, indicating that I₃QGK nanofibers not only promote cellular attachment but also facilitate differentiation into neuronal phenotypes. Inkjet printing allows complex patterning of peptide nanofibers onto RSF substrates, which enabled us to engineer cell alignment and provide an opportunity to direct axonal development in vitro. The live/dead assay showed that printed I₃QGK patterns exhibit no cytotoxicity to PC12 cells demonstrating potential for future nerve tissue engineering applications.     

水泥基快硬瓷砖胶中铝酸盐复合胶凝体系的试验

通过改变普通硅酸盐水泥与铝酸盐水泥二元体系的比例,测试瓷砖胶的早期、水养和热养拉伸胶粘强度的变化趋势,研究两种三元胶凝材料体系对瓷砖胶拉伸胶粘强度的影响.结果表明:以铝酸盐水泥为主的胶凝材料可满足胶粘强度技术要求.最后,结合其水化结果进行机理分析.     

Modelling for Evaluation of Tensile Strength of Nonwoven and Its Experi-mental Verification

In this paper,the critical zone concept is proposed onthe mechanism of tensile breakage of nonwoven.Thestrength of critical zone of nonwoven is taken to be allthe pull-out force of bridging fibers from nonwoven,which are related to content of adhesive.The strength ofnonwoven is determined by the strength of critical zone.Based on these,a mathematical model of nonwovenstrength was derived.     

声发射技术检测金属胶接结构性能探讨

介绍了声发射检测的基本原理,提出了用声发射技术检测金属胶接结构的两种实验方法,并对实验结果进行了分析结果表明：声发射技术是检测金属胶接结构的有效方法,声信号的幅度分布及发生次数与胶接结构性能有密切关系     

我国食品塑料复合软包装用印刷油墨的安全问题及对策

介绍我国食品塑料复合软包装用印刷油墨行业概况,从该类油墨在安全方面的突出问题、产生原因等进行分析,提出了我国食品塑料复合软包装用印刷油墨质量安全对策与建议.     

Aldehyde-Sodium Alginate and Amino-Gelatin Preparation as Soft Tissue Adhesive

Sodium alginate and gelatin are both remarkable natural biomaterials; they all have been extensively applied in tissue engineering and other relative fields,due to their low price and good biocompatibility. In this paper,we oxidized sodium alginate with sodium periodate to convert 1,2-hydroxyl groups into aldehyde groups to get aldehyde-sodium alginate（ A-SA）. Gelatin was modified with ethylenediamine（ ED） in the presence of water-soluble1-ethyl-3（ 3-dimethylaminopropyl） carbodiimide（ EDC） to introduce additional amino groups to get amino-gelatin. Upon mixing the A-SA and amino-gelatin aqueous solutions together,a gel rapidly formed based on the Schiff＇s base reaction between the aldehyde groups in A-SA and the amino groups in amino-gelatin.Fourier transform infrared spectroscopy（ FTIR） analysis confirmed the characteristic peak of Schiff＇s base group in the hydrogel. The gelation time measure has confirmed the gelation time is dependent on the aldehyde group content in A-SA and amino group content in amino-gelatin. The fasted hydrogel formation takes place within 30 s. The entire test suggested that this gel could be a promising candidate as soft tissue adhesive.     

桂花品种SSR荧光指纹图谱的构建

利用SSR荧光标记技术筛选出的11对SSR荧光标记引物,构建了64个桂花品种的SSR指纹图谱。11对SSR引物共扩增出143个等位基因,平均每个位点13个等位基因。群体平均的观测杂合度(Ho)、期望杂合度(He)、Shannon's信息指数(I)、观察等位基因数(Na)和有效等位基因数(Ne)分别为0.619 7、0.848 3、2.323 7、17.833 3和8.228 2。四季桂与秋桂(金桂、银桂和丹桂)的遗传距离较远。利用引物OF002、OF019、OSM63和OSM63中的任意2对可区分所有供试品种。64个桂花品种的SSR指纹图谱互不相同,可以作为各品种特定的图谱,为品种鉴别提供依据。     

基于ANSYS CFX的三维打印喷头结构优化设计

喷头结构是影响三维打印成形效果的主要因素。通过Pro/e建立了喷头的三维模型,利用ANSYS CFX对其流场进行了有限元分析并优化了结构参数。结果表明,出口直径一定时,入口直径越小,喷头所受到的压力越小,流动性能越好;收敛角在小于90°时,收敛角越小越有利于流体流动,收敛角大于90°时,角度越大阻力小幅度减小;过渡圆弧半径越大,流体流动状态越好。     

我国书籍形态构成的演变与发展

从我国不同时期书籍形态构成的演变中, 探索由传统书籍形态信息传递的单向性向书籍形态设计构成多向性的变化趋势。     

扫描电镜在J-116结构胶粘剂与QY8911树脂匹配性研究中的应用

本文利用扫描电镜观察固化后的J－116、648、LWF－1及QY8911树脂的断口形态,以及J－116／648、J－116／LWF－1和J－116／QY8911的界面断口形态,由断口形态知,J－116结构胶粘剂与QY8911树脂具有较好的匹配性。